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Human Corneal Endothelium: Growth in Tissue Cultures

Human Corneal Endothelium: Growth in Tissue Cultures Abstract The primary culture of rabbit corneal endothelium after isolation by trypsinization has been reported.1 Although this technique was uniformly successful in rabbits and monkeys, identical experiments upon human eyes failed with rare exception. A modification in technique has afforded success in 18 consecutive experiments on human corneas and, therefore, is thought worthy of reporting. This paper describes the modified technique for isolation and the primary growth of human corneal endothelium in tissue culture and presents observations of cell activity and the appearance of the endothelial mitochondria. A brief summary of the isolation and growth techniques which were unsuccessful is also reported. Materials and Methods The studies utilized human eyes which were not required for corneal transplantation. The eyes, varying from 28 to 70 years-of-age, were removed under surgically aseptic conditions within six hours after death, and washed with 0.5% ophthalmic Neosporin* solution. Cell culture was established 24 to 72 References 1. A solution of polymyxin B sulfate, neomycin sulfate, and gramicidin. 2. Slick, W.C.; Mannagh, J.; and Yuhasz, Z.: Enzymatic Removal and Pure Culture of Rabbit Corneal Endothelial Cells , Arch Ophthal 73:229, 1965.Crossref 3. Gwatkin, R.B.L., and Thompson, G.: New Method for Dispersing the Cells of Mammalian Tissues , Nature 201: 4925, 1964.Crossref 4. Polak, M.: Sobre una tecnica sencilla y rapida para la coloración del condrioma , Arch Histol 3:365-376, 1946. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Human Corneal Endothelium: Growth in Tissue Cultures

Archives of Ophthalmology , Volume 74 (6) – Dec 1, 1965

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Publisher
American Medical Association
Copyright
Copyright © 1965 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1965.00970040849023
Publisher site
See Article on Publisher Site

Abstract

Abstract The primary culture of rabbit corneal endothelium after isolation by trypsinization has been reported.1 Although this technique was uniformly successful in rabbits and monkeys, identical experiments upon human eyes failed with rare exception. A modification in technique has afforded success in 18 consecutive experiments on human corneas and, therefore, is thought worthy of reporting. This paper describes the modified technique for isolation and the primary growth of human corneal endothelium in tissue culture and presents observations of cell activity and the appearance of the endothelial mitochondria. A brief summary of the isolation and growth techniques which were unsuccessful is also reported. Materials and Methods The studies utilized human eyes which were not required for corneal transplantation. The eyes, varying from 28 to 70 years-of-age, were removed under surgically aseptic conditions within six hours after death, and washed with 0.5% ophthalmic Neosporin* solution. Cell culture was established 24 to 72 References 1. A solution of polymyxin B sulfate, neomycin sulfate, and gramicidin. 2. Slick, W.C.; Mannagh, J.; and Yuhasz, Z.: Enzymatic Removal and Pure Culture of Rabbit Corneal Endothelial Cells , Arch Ophthal 73:229, 1965.Crossref 3. Gwatkin, R.B.L., and Thompson, G.: New Method for Dispersing the Cells of Mammalian Tissues , Nature 201: 4925, 1964.Crossref 4. Polak, M.: Sobre una tecnica sencilla y rapida para la coloración del condrioma , Arch Histol 3:365-376, 1946.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Dec 1, 1965

References