Abstract • A characteristic and natural autofluorescence that appears brilliant green in frozen sections of untreated skin when viewed under the fluorescent microscope is demonstrated. Study with various barrier filters and exciter filters indicates that the optimum absorption for visualization of this is 300 to 330 nm, and the optimum fluorescence is between 500 and 530 nm under the conditions used. Clinical study of skin from 52 patients including black, white, Puerto Rican, and Chinese indicates a relationship between skin color and intensity of autofluorescence. In addition, the cellular localization of autofluorescence corresponds to sites of melanin. Cells in the basal layer are involved most conspicuously and nuclei are spared. (Arch Dermatol 112:667-670, 1976) References 1. Dunn DR, Barth RF: Identification of melanoma cells by formaldehyde-induced fluorescence . Cancer 33:701-706, 1974.Crossref 2. Falck B, Jacobson S, Olivecrona H, et al: Fluorescent dopa reaction of nevi and melanomas . Arch Dermatol 94:363-369, 1966.Crossref 3. Olivecrona H, Rorsman H: The effect of roentgen irradiation on the specific fluorescence of epidermal melanocytes . Acta Derm Venereol 46:403-405, 1966. 4. Olivecrona H, Rorsman H: Fluorescence microscopy of malignant melanoma in the syrian golden hamster . Acta Derm Venereol 46:401-402, 1966. 5. Hamperl H: Die Fluoreszenzmikroskopie Menschlicher Gewebe . Virchows Arch Pathol Anat 292:1-51, 1934.Crossref 6. Van Duijn C: Primary fluorescence . Microscope 10:1-6, 1954. 7. De Lerma B: Die Anwendung Von Fluoreszenlicht in der Histochemic , in Graumann W, Neumann K (eds): Handbuch der histochemic . 1/ (1) , 78-159, 1958. 8. Nairn RC: Fluorescent Protein Tracing , ed 2. Baltimore, Williams & Wilkins Co, 1964, p 7.
Archives of Dermatology – American Medical Association
Published: May 1, 1976