Abstract Cells derived from a retinoblastoma from a 3-year-old boy have been cultured for two years; these cells have no glutamine synthetase activity. Evidence is presented that a normal human retina of this age should have appreciable amounts of this enzyme activity. The tumor derived cells can be induced for the enzyme by 14 or more days treatment with 0.5μg to 1.0μg/ml of cortisol. References 1. Rudnick D, Waelsch HJ: Development of glutamotransferase and glutamine synthetase in the nervous system of the chick . Exp Zool 129:309, 1955.Crossref 2. Moscona AA, Kirk DL: Control of glutamine synthetase in the embryonic retina in vitro . Science 148:519, 1965.Crossref 3. Reif L, Amos H: A dialyzable inducer for the glutamotransferase of chick embryo retina . Biochem Biophys Res Comm 23:39, 1966.Crossref 4. Moscona AA, Piddington R: Stimulation by hydrocortisone of premature changes in the developmental pattern of glutamine synthetase in embryonic retina . Biochim Biophys Acta 121:409, 1966.Crossref 5. Reif-Lehrer L, Amos H: Hydrocortisone requirement for the induction of glutamine synthetase in chick-embryo retinas . Biochem J 106:425, 1968. 6. Wu C: Glutamine synthetase: I. A comparative study of its distribution in animals and its inhibition by DL-allo-δ-hydroxylysine . Comp Biochem Physiol 8:335, 1963.Crossref 7. Lund P, Goldstein L: Glutamine synthetase activity in tissues of lower vertebrates . Comp Biochem Physiol 31:205, 1969.Crossref 8. Piddington R, Moscona AA: Correspondence between glutamine synthetase activity and differentiation in the embryonic retina in situ and in culture . J Cell Biol 27:247, 1965.Crossref 9. Sheffield JB, Fischman DA: Intercellular junctions in the developing neural retina of the chick embryo . Z Zellforsch 104:405, 1970.Crossref 10. Amos H, Moore MO: The influence of bacterial ribonucleic acid on animal cells in culture . Exp Cell Res 32:1, 1963.Crossref 11. Waelsch H: Glutamotransferase (α-glutamyltransferase) , Colowick SP, Kaplan NO (eds): Methods in Enzymology . New York, Academic Press, 1955, vol 2, p 267. 12. Thorndike J, Reif-Lehrer L: A sensitive assay for glutamyltransferase. Enzym Biol Clin, to be published. 13. Lowry OH, Rosebrough NJ, Farr AL, et al: Protein measurement with the folin phenol reagent . J Biol Chem 193:265, 1951. 14. Albert DM, Rabson AS, Dalton AJ: Tissue culture study of human retinoblastoma . Invest Ophthal 9:64, 1970. 15. Chader GJ, Saunders LR, Reif-Lehrer L: Glutamine synthetase in the normal human retina . Arch Ophthal 85:286-287, 1971.Crossref 16. Mann I: The Development of the Human Eye . New York, Grune & Stratton Inc, 1964. 17. Morris JE, Moscona AA: Induction of glutamine synthetase in embryonic retina: Its dependence on cell interactions . Science 167:1736, 1970.Crossref 18. De Mar R: The inhibition by glutamine of glutamyl-transferase formation in cultures of human cells . Biochim Biophys Acta 27:435, 1958.Crossref 19. Barnes PR, Youngberg D, Kitos PA: Factors affecting the production of glutamine in cultured mouse cells. J Cell Physiol, to be published.
Archives of Ophthalmology – American Medical Association
Published: Jul 1, 1971
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera