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Fluorescent Antibody Techniques: Methods for Identification of Bordetella Pertussis

Fluorescent Antibody Techniques: Methods for Identification of Bordetella Pertussis Abstract The diagnosis of early, mild, or atypical whooping cough is dependent upon identification of the etiologic agent. Although procedures for the isolation of Bordetella pertussis1 have been available for many years and are of proven diagnostic aid, they have not gained the wide usage they seem to deserve. A recognized limitation is the time needed: 3, more often 4, and occasionally more, days are required for a positive report. The possibility of using fluorescent antibody methods for rapid identification of B. pertussis in nasopharyngeal specimens is suggested by the promising results reported in recent studies of a number of bacterial infections. For example, Group A streptococci,2,3 enteropathogenic Escherichia coli,4 Haemophilus influenzae, Type B,5 Neisseria gonorrhoeae,6 Vibrio comma,7 Leptospira,8 and certain other bacterial agents have been demonstrated by means of this technique in specimens taken directly from the patient. The work of de Repentigny References 1. Kendrick, P. L.; Lawson, G. McL., and Miller, J. J.: Hemophilus Pertussis , Chapter in Diagnostic Procedures and Reagents , Ed. 3, American Public Health Association, 1790 Broadway, New York, 1950. 2. Moody, M. D.; Ellis, E. C., and Updyke, E. L.: Grouping Streptococci in Dried Smears with Fluorescent Antibody , Bact. Proc. 1958, p. 135. 3. Wolfe, M. D., and Cameron, G. M.: Fluorescent Antibody Technique Versus Cultural Methods as a Routine Procedure for Finding Beta-Hemolytic Streptococci in Throat Cultures , Pub. Health Lab. 17:76-82, 1959. 4. Whitaker, J.; Page, R. H.; Stulberg, C. S., and Zuelzer, W. W.: Rapid Identification of Enteropathogenic Escherichia Coli 0127: B8 by the Fluorescent Antibody Technique , A.M.A.J. Dis. Child. 95:1-8, 1958. 5. Page, R. H.; Caldroney, G. L., and Stulberg, C. S.: Immunofluorescence in Diagnostic Bacteriology: I. Direct Identification of Hemophilus influenzae in Smears of Cerebrospinal Fluid Sediments, Am. J. Dis. Child., to be published. 6. Deacon, W. E.; Peacock, W. L.; Freeman, E. M.; Harris, A., and Bunch, W. L.: Fluorescent Antibody Tests for Detection of the Gonococcus in Women , Pub. Health Rep. 75:125-129, 1960.Crossref 7. Finkelstein, R. A., and La Brec, E. H.: Rapid Identification of Cholera Vibrios with Fluorescent Antibody , J. Bact. 78:886-891, 1959. 8. White, F. H., and Ristic, M.: Detection of Leptospira Pomoma in Guinea Pig and Bovine Urine with Fluorescein-Labeled Antibody , J. Infect. Dis. 105:118-123, 1959.Crossref 9. de Repentigny, J., and Frappier, A.: Studies on H. Pertussis Liquid Cultures: III. Localization of Surface Antigens by Means of Fluorescent Antibody , Canad. J. Microbiol. 2:677-683, 1956.Crossref 10. Donaldson, P., and Whitaker, J. A.: Diagnosis of Pertussis by Fluorescent Antibody Staining of Nasopharyngeal Smears , A.M.A.J. Dis. Child. 99:423-427, 1960. 11. Brooks, A. M.; Bradford, W. L., and Berry, G. P.: The Method of Nasopharyngeal Culture in the Diagnosis of Whooping Cough , J.A.M.A. 120:883-885, 1942.Crossref 12. Eldering, G.; Hornbeck, C., and Baker, J.: Serological Study of Bordetella Pertussis and Related Species , J. Bact. 74:133-136, 1957.Crossref 13. Kendrick, P. L.; Updyke, E. L., and Eldering, G.: Comparison of Pertussis Cultures by Mouse Protection and Virulence Tests , Am. J. Pub. Health 39:179-184, 1949.Crossref 14. Riggs, J. L.; Seiwald, R. J.; Burckhalter, J. H.; Downs, C. M., and Metcalf, T. G.: Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum , Am. J. Path. 34: 1081-1097, 1958. 15. Marshall, J. D.; Eveland, W. C., and Smith, C. W.: Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of Its Application , Proc. Soc. Exper. Biol. & Med. 98:898-900, 1958. 16. Smith, C. W.; Marshall, J. D., and Eveland, W. C.: Use of Contrasting Fluorescent Dye as a Counterstain in Fixed Tissue Preparations , Proc. Soc. Exper. Biol. & Med. 102:179-181, 1959. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Fluorescent Antibody Techniques: Methods for Identification of Bordetella Pertussis

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References (15)

Publisher
American Medical Association
Copyright
Copyright © 1961 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1961.04020030013003
Publisher site
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Abstract

Abstract The diagnosis of early, mild, or atypical whooping cough is dependent upon identification of the etiologic agent. Although procedures for the isolation of Bordetella pertussis1 have been available for many years and are of proven diagnostic aid, they have not gained the wide usage they seem to deserve. A recognized limitation is the time needed: 3, more often 4, and occasionally more, days are required for a positive report. The possibility of using fluorescent antibody methods for rapid identification of B. pertussis in nasopharyngeal specimens is suggested by the promising results reported in recent studies of a number of bacterial infections. For example, Group A streptococci,2,3 enteropathogenic Escherichia coli,4 Haemophilus influenzae, Type B,5 Neisseria gonorrhoeae,6 Vibrio comma,7 Leptospira,8 and certain other bacterial agents have been demonstrated by means of this technique in specimens taken directly from the patient. The work of de Repentigny References 1. Kendrick, P. L.; Lawson, G. McL., and Miller, J. J.: Hemophilus Pertussis , Chapter in Diagnostic Procedures and Reagents , Ed. 3, American Public Health Association, 1790 Broadway, New York, 1950. 2. Moody, M. D.; Ellis, E. C., and Updyke, E. L.: Grouping Streptococci in Dried Smears with Fluorescent Antibody , Bact. Proc. 1958, p. 135. 3. Wolfe, M. D., and Cameron, G. M.: Fluorescent Antibody Technique Versus Cultural Methods as a Routine Procedure for Finding Beta-Hemolytic Streptococci in Throat Cultures , Pub. Health Lab. 17:76-82, 1959. 4. Whitaker, J.; Page, R. H.; Stulberg, C. S., and Zuelzer, W. W.: Rapid Identification of Enteropathogenic Escherichia Coli 0127: B8 by the Fluorescent Antibody Technique , A.M.A.J. Dis. Child. 95:1-8, 1958. 5. Page, R. H.; Caldroney, G. L., and Stulberg, C. S.: Immunofluorescence in Diagnostic Bacteriology: I. Direct Identification of Hemophilus influenzae in Smears of Cerebrospinal Fluid Sediments, Am. J. Dis. Child., to be published. 6. Deacon, W. E.; Peacock, W. L.; Freeman, E. M.; Harris, A., and Bunch, W. L.: Fluorescent Antibody Tests for Detection of the Gonococcus in Women , Pub. Health Rep. 75:125-129, 1960.Crossref 7. Finkelstein, R. A., and La Brec, E. H.: Rapid Identification of Cholera Vibrios with Fluorescent Antibody , J. Bact. 78:886-891, 1959. 8. White, F. H., and Ristic, M.: Detection of Leptospira Pomoma in Guinea Pig and Bovine Urine with Fluorescein-Labeled Antibody , J. Infect. Dis. 105:118-123, 1959.Crossref 9. de Repentigny, J., and Frappier, A.: Studies on H. Pertussis Liquid Cultures: III. Localization of Surface Antigens by Means of Fluorescent Antibody , Canad. J. Microbiol. 2:677-683, 1956.Crossref 10. Donaldson, P., and Whitaker, J. A.: Diagnosis of Pertussis by Fluorescent Antibody Staining of Nasopharyngeal Smears , A.M.A.J. Dis. Child. 99:423-427, 1960. 11. Brooks, A. M.; Bradford, W. L., and Berry, G. P.: The Method of Nasopharyngeal Culture in the Diagnosis of Whooping Cough , J.A.M.A. 120:883-885, 1942.Crossref 12. Eldering, G.; Hornbeck, C., and Baker, J.: Serological Study of Bordetella Pertussis and Related Species , J. Bact. 74:133-136, 1957.Crossref 13. Kendrick, P. L.; Updyke, E. L., and Eldering, G.: Comparison of Pertussis Cultures by Mouse Protection and Virulence Tests , Am. J. Pub. Health 39:179-184, 1949.Crossref 14. Riggs, J. L.; Seiwald, R. J.; Burckhalter, J. H.; Downs, C. M., and Metcalf, T. G.: Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum , Am. J. Path. 34: 1081-1097, 1958. 15. Marshall, J. D.; Eveland, W. C., and Smith, C. W.: Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of Its Application , Proc. Soc. Exper. Biol. & Med. 98:898-900, 1958. 16. Smith, C. W.; Marshall, J. D., and Eveland, W. C.: Use of Contrasting Fluorescent Dye as a Counterstain in Fixed Tissue Preparations , Proc. Soc. Exper. Biol. & Med. 102:179-181, 1959.

Journal

American Journal of Diseases of Children – American Medical Association

Published: Feb 1, 1961

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