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Fluorescent Antibody Localization of Herpes Simplex Virus in the Conjunctiva

Fluorescent Antibody Localization of Herpes Simplex Virus in the Conjunctiva Abstract Introduction Experimental herpes simplex infection of the rabbit eye resembles primary ocular herpes in man. As in man, conjunctivitis is often associated with the keratitis and may be more severe than the keratitis. Evidence for the presence of virus infection in the cornea has been well documented over the years. Scrapings from the dendritic lesions show the viral type of giant cells. Intranuclear inclusions are not usually visible in epithelial scrapings but can be seen if the cornea is sectioned. Specific localization of the viral antigen can be regularly demonstrated by staining the epithelial scrapings with specific fluorescein-tagged antisera (FA stain).1-3 Recently Pettit et al4 correlated this localization of the antigen by FA staining with its localization by the electron microscope.Although the conjunctivas of eyes inoculated with herpes virus become inflamed, definite conjunctival lesions have not been clinically demonstrable. Experimentally, however, a few observers—notably Goodpasture and Teague References 1. Kaufman, H. E.: Diagnosis of Corneal Herpes Simplex Infection by Fluorescent Antibody Staining , Arch Ophthal 64:382-384, 1960.Crossref 2. Vozza, R., and Balducci, D.: Technique of Fluorescent Antibody in Ophthalmology: Study of Herpes Simplex and Vaccine Keratoconjunctivitis and Human Trachomatous Infection , Amer J Ophthal 52:72-77, 1961. 3. Witmer, R.: Fluoresceierende Antikörper in der Diagnose herpetischer Erkrankung , Ophthalmologica 141:278-282, 1961.Crossref 4. Pettit, T. H.; Kimura, S. J.; and Peters, H.: Fluorescent Antibody Technique in Diagnosis of Herpes Simplex Keratitis , Arch Ophthal 72:86-98, 1964.Crossref 5. Goodpasture, E. W., and Teague, O.: Experimental Production of Herpetic Lesions in Organs and Tissues of Rabbit , J Med Res 44:121-138, 1923. 6. Crouse, H. D., et al: Cytochemical Studies on Intranuclear Inclusion of Herpes Simplex , J Immun 65:119-128, 1950. 7. Lebrum, J.: Cellular Localization of Herpes Simplex Virus With Fluorescent Antibody , Virology 2:496-510, 1956.Crossref 8. Sheldon, W. H.: Leptospiral Antigen Demonstrated by Fluorescent Antibody Technic in Human Lesions of Leptospirosis Icterohemorrhagiae , Proc Soc Exp Biol Med 84:165-167, 1955.Crossref 9. Coons, A. H.: " Fluorescent Antibody Methods ," in General Cytochemical Methods , J. F. Danielli, ed., New York: Academic Press, Inc., 1958, vol 1, p 420. 10. Garabedian, G. A., and Syverton, J. T.: Studies on Herpes Simplex Virus: II. Affinity in Vitro of Herpes Virus for Rabbit Microphages and Macrophages , J Infect Dis 96:5-8, 1955.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Fluorescent Antibody Localization of Herpes Simplex Virus in the Conjunctiva

Archives of Ophthalmology , Volume 73 (3) – Mar 1, 1965

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Publisher
American Medical Association
Copyright
Copyright © 1965 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1965.00970030415021
Publisher site
See Article on Publisher Site

Abstract

Abstract Introduction Experimental herpes simplex infection of the rabbit eye resembles primary ocular herpes in man. As in man, conjunctivitis is often associated with the keratitis and may be more severe than the keratitis. Evidence for the presence of virus infection in the cornea has been well documented over the years. Scrapings from the dendritic lesions show the viral type of giant cells. Intranuclear inclusions are not usually visible in epithelial scrapings but can be seen if the cornea is sectioned. Specific localization of the viral antigen can be regularly demonstrated by staining the epithelial scrapings with specific fluorescein-tagged antisera (FA stain).1-3 Recently Pettit et al4 correlated this localization of the antigen by FA staining with its localization by the electron microscope.Although the conjunctivas of eyes inoculated with herpes virus become inflamed, definite conjunctival lesions have not been clinically demonstrable. Experimentally, however, a few observers—notably Goodpasture and Teague References 1. Kaufman, H. E.: Diagnosis of Corneal Herpes Simplex Infection by Fluorescent Antibody Staining , Arch Ophthal 64:382-384, 1960.Crossref 2. Vozza, R., and Balducci, D.: Technique of Fluorescent Antibody in Ophthalmology: Study of Herpes Simplex and Vaccine Keratoconjunctivitis and Human Trachomatous Infection , Amer J Ophthal 52:72-77, 1961. 3. Witmer, R.: Fluoresceierende Antikörper in der Diagnose herpetischer Erkrankung , Ophthalmologica 141:278-282, 1961.Crossref 4. Pettit, T. H.; Kimura, S. J.; and Peters, H.: Fluorescent Antibody Technique in Diagnosis of Herpes Simplex Keratitis , Arch Ophthal 72:86-98, 1964.Crossref 5. Goodpasture, E. W., and Teague, O.: Experimental Production of Herpetic Lesions in Organs and Tissues of Rabbit , J Med Res 44:121-138, 1923. 6. Crouse, H. D., et al: Cytochemical Studies on Intranuclear Inclusion of Herpes Simplex , J Immun 65:119-128, 1950. 7. Lebrum, J.: Cellular Localization of Herpes Simplex Virus With Fluorescent Antibody , Virology 2:496-510, 1956.Crossref 8. Sheldon, W. H.: Leptospiral Antigen Demonstrated by Fluorescent Antibody Technic in Human Lesions of Leptospirosis Icterohemorrhagiae , Proc Soc Exp Biol Med 84:165-167, 1955.Crossref 9. Coons, A. H.: " Fluorescent Antibody Methods ," in General Cytochemical Methods , J. F. Danielli, ed., New York: Academic Press, Inc., 1958, vol 1, p 420. 10. Garabedian, G. A., and Syverton, J. T.: Studies on Herpes Simplex Virus: II. Affinity in Vitro of Herpes Virus for Rabbit Microphages and Macrophages , J Infect Dis 96:5-8, 1955.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Mar 1, 1965

References

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