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Fibronectin Enhances Healing of Excised Wounds in Rats

Fibronectin Enhances Healing of Excised Wounds in Rats Abstract • Fibronectin (Fn) is a normal plasma and extracellular matrix glycoprotein that is involved in each phase of wound healing. For example, it is incorporated into both fibrin and collagen fibers; it opsonizes circulating tissue debris for removal by the reticuloendothelial system; it is used by macrophages, fibroblasts, and epithelial cells to move into the wound; and fragments of Fn are chemotactic for fibroblasts. In this study, experiments with rats showed that excised lesions treated with Fn healed more rapidly than paired control lesions treated with the carrier alone. Applications of Fn once a day for two days were as effective in speeding healing as twice-daily applications of Fn for 12 days. A single treatment with Fn soon after the initial injury was nearly as effective as more prolonged treatment regimens. (Arch Dermatol 1988;124:221-225) References 1. Evans P: The healing process at the cellular level: A review . Physiotherapy 1980;20:256-259. 2. Grinnell F: Fibronectin and wound healing . J Cell Biochem 1984;26:107-116.Crossref 3. Reese AC, Doran JE, Raynor RH, et al: Role of fibronectin in wound healing . Recent Adv Oral Maxillofacial Surg 1983;4:1-25. 4. Mosher DF: Fibronectin . Prog Hemost Thromb 1980;5:111-151. 5. Mosher DF, Schad PE, Vann JM: Cross-linking of collagen and fibronectin by factor XIIIa . J Biol Chem 1980;255:1181-1188. 6. Benacerraf B, Bioppi G, Halpren BN, et al: Physiology of phagocytosis of particles by the RES , in Halpren BN (ed): Physiology of the Reticuloendothelial System . Springfield, Ill, Charles C Thomas Publisher, 1975, pp 52-75. 7. Rogers FB, Sheaff CM, Nolan PJ, et al: Fibronectin depletion and microaggregate clearance following trauma . J Trauma 1986;26:339-42.Crossref 8. Powell JT, Poskitt KR, Irwin JT, et al: Opsonic dysfunction secondary to plasma fibronectin depletion after aortic surgery . Br J Surg 1986;73:38-40.Crossref 9. Martin DE, Reece MC, Maher JE, et al: Tissue debris at the injury site is coated by plasma fibronectin and subsequently removed by tissue macrophages . Arch Dermatol 1988;124: 226-229.Crossref 10. Postlewaite AE, Keski-Oja J, Balian G, et al: Induction of fibroblast chemotaxis by fibronectin . J Exp Med 1981;153:494-499.Crossref 11. Norris DA, Clark RAF, Swigart LM, et al: Fibronectin fragment(s) are chemotactic for human peripheral blood monocytes . J Immunol 1982;129:1612-1618. 12. Peacock EE Jr, van Winkle W Jr: Structure, synthesis and interaction of fibrous protein and matrix , in Peacock EE, van Winkle W Jr (eds): Wound Repair , ed 2. Philadelphia, WB Saunders Co, 1976, p 81. 13. Knox P, Crooks S, Rimer CS: Role of fibronectin in the migration of fibroblasts into plasma clots . J Cell Biol 1986; 102:2318-2323.Crossref 14. Clark RA, Lanigan JM, Dellapella P, et al: Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization . J Invest Dermatol 1983;799:264-269. 15. Donaldson DJ, Malhan JT: Fibrinogen and fibronectin as substrates for epidermal cell migration during wound closure . J Cell Sci 1983;62:117-127. 16. Baur PS, Parks DH: The myofibroblast anchoring strand: The fibronectin connection in wound healing and the possible loci of collagen fibril assembly . J Trauma 1983;23:853-862.Crossref 17. McDonagh J: Fibronectin: A molecular glue . Arch Pathol Lab Med 1981;105:393-396. 18. Mosher DF: Physiology of fibronectin . Ann Rev Med 1984;35:561-575.Crossref 19. Giancotti FG, Comoglio PM, Tarone G: Fibronectin-plasma membrane interaction in the adhesion of hemopoietic cells . J Cell Biol 1986;103:429-437.Crossref 20. Hosein B, Bianco C: Monocyte receptors for fibronectin characterized by monoclonal antibody that interferes with receptor activity . J Exp Med 1985;162:157-170.Crossref 21. Reese AC, Doran JE, Callaway BD, et al: Sequestration of fibronectin at the site of an injury . Adv Shock Res 1982;8:119-127. 22. Lanser ME, Saba TM, Scovill W: Opsonic glycoprotein (plasma fibronectin) levels after burn injury: Relationship to extent of burn and development of sepsis . Ann Surg 1980;192:776-789.Crossref 23. Dionyssiou-Asteriou A, Karavassilis J: Level of plasma fibronectin in patients with vascular disease . Clin Chim Acta 1985;152:79-83.Crossref 24. Richards WO, Scovill WA, Shin B: Opsonic fibronectin deficiency in patients with intra-abdominal infection . Surgery 1983;94:210-217. 25. Lanser ME, Saba TM, Scovill W: Opsonic glycoprotein (plasma fibronectin) levels after burn injury: Relationship to extent of burn and development of sepsis . Ann Surg 1980;192:776-789.Crossref 26. Saba TM, DiLuzio NA: Reticuloendothelial blockade and recovery as a function of opsonic activity . Am J Physiol 1969;216:197-205. 27. Lundsgaard-Hansen P, Doran JE, Rubli E, et al: Purified fibronectin administration to patients with severe abdominal infections: A controlled study . Ann Surg 1985;202:745-759.Crossref 28. Saba TM, Blumenstock FA, Bernard H: Cryoprecipitate reversal of opsonic alpha-2-surface binding glycoprotein deficiencies in septic surgical trauma patients . Science 1978;201:622-624.Crossref 29. Doran JE, Mansberger AR, Reese AC: Cold insoluble globulin-enhanced phagocytosis of gelatinized targets by macrophage monolayers . J Reticuloendothel Soc 1980;27:471-483. 30. Ruoslahti E, Hayman EG, Piershbacher M, et al: Fibronectin: Purification, immunochemical properties, and biological activities . Methods Enzymol 1982;82:803-831. 31. Kono I, Matsumoto Y, Kono K, et al: Beneficial effects of topical fibronectin in patients with keratoconjunctivitis sicca of Sjogren's syndrome . J Rheumatol 1985;12:487-489. 32. Nishida T, Nakagawa S, Nishibayashi C, et al: Fibronectin enhancement of corneal epithelial wound healing of rabbits in vivo . Arch Ophthalmol 1984;102:455-457.Crossref 33. Rubin LF: Toxicologic update of dimethyl sulfoxide . Ann NY Acad Sci 1983;411:6-10.Crossref 34. Jacob SW, Herschler RJ, Rosenbaum EE: Dimethyl sulfoxide (DMSO): Laboratory and clinical evaluation . J Am Vet Med Assoc 1965;147:1350-1359. 35. Shirley SW, Stewart BH, Mirelman A: Dimethyl sulfoxide in treatment of inflammatory genitourinary disorders . Urology 1978;11:215-220.Crossref 36. Rao CV: Differential effects of detergents and dimethyl sulfoxide on membrane prostaglandin E and F2a receptors . Life Sci 1977;20:2013-2022.Crossref 37. Wood DC, Wood J: Pharmacologic and biochemical considerations of dimethylsulfoxide . Ann NY Acad Sci 1975:243:7-19.Crossref 38. Goldblum OM, Alvarez OM, Mertz MM, et al: Dimethyl sulfoxide (DMSO) does not affect epidermal wound healing . Proc Soc Exp Biol Med 1983;172:301-307.Crossref 39. Rollins BHJ, Cathcart MK, Culp LA: Fibronectin proteoglycan binding as the molecular basis for fibroblast adhesion to extracellular matrices , in Howowitz MI (ed): The Glycoconjugates . Orlando, Fla, Academic Press Inc, 1982, vol 3, pp 289-329. 40. Grinnell F, Feld M, Minter D: Fibroblast adhesion to fibrinogen and fibrin substrata: Requirement of cold-insoluble globulin (plasma fibronectin) . Cell 1980;19:517-528.Crossref 41. Peacock EE Jr: Wound Repair , ed 3. Philadelphia, WB Saunders Co, 1984, pp 38-55. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Fibronectin Enhances Healing of Excised Wounds in Rats

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Publisher
American Medical Association
Copyright
Copyright © 1988 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1988.01670020039014
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Abstract

Abstract • Fibronectin (Fn) is a normal plasma and extracellular matrix glycoprotein that is involved in each phase of wound healing. For example, it is incorporated into both fibrin and collagen fibers; it opsonizes circulating tissue debris for removal by the reticuloendothelial system; it is used by macrophages, fibroblasts, and epithelial cells to move into the wound; and fragments of Fn are chemotactic for fibroblasts. In this study, experiments with rats showed that excised lesions treated with Fn healed more rapidly than paired control lesions treated with the carrier alone. Applications of Fn once a day for two days were as effective in speeding healing as twice-daily applications of Fn for 12 days. A single treatment with Fn soon after the initial injury was nearly as effective as more prolonged treatment regimens. (Arch Dermatol 1988;124:221-225) References 1. Evans P: The healing process at the cellular level: A review . Physiotherapy 1980;20:256-259. 2. Grinnell F: Fibronectin and wound healing . J Cell Biochem 1984;26:107-116.Crossref 3. Reese AC, Doran JE, Raynor RH, et al: Role of fibronectin in wound healing . Recent Adv Oral Maxillofacial Surg 1983;4:1-25. 4. Mosher DF: Fibronectin . Prog Hemost Thromb 1980;5:111-151. 5. Mosher DF, Schad PE, Vann JM: Cross-linking of collagen and fibronectin by factor XIIIa . J Biol Chem 1980;255:1181-1188. 6. Benacerraf B, Bioppi G, Halpren BN, et al: Physiology of phagocytosis of particles by the RES , in Halpren BN (ed): Physiology of the Reticuloendothelial System . Springfield, Ill, Charles C Thomas Publisher, 1975, pp 52-75. 7. Rogers FB, Sheaff CM, Nolan PJ, et al: Fibronectin depletion and microaggregate clearance following trauma . J Trauma 1986;26:339-42.Crossref 8. Powell JT, Poskitt KR, Irwin JT, et al: Opsonic dysfunction secondary to plasma fibronectin depletion after aortic surgery . Br J Surg 1986;73:38-40.Crossref 9. Martin DE, Reece MC, Maher JE, et al: Tissue debris at the injury site is coated by plasma fibronectin and subsequently removed by tissue macrophages . Arch Dermatol 1988;124: 226-229.Crossref 10. Postlewaite AE, Keski-Oja J, Balian G, et al: Induction of fibroblast chemotaxis by fibronectin . J Exp Med 1981;153:494-499.Crossref 11. Norris DA, Clark RAF, Swigart LM, et al: Fibronectin fragment(s) are chemotactic for human peripheral blood monocytes . J Immunol 1982;129:1612-1618. 12. Peacock EE Jr, van Winkle W Jr: Structure, synthesis and interaction of fibrous protein and matrix , in Peacock EE, van Winkle W Jr (eds): Wound Repair , ed 2. Philadelphia, WB Saunders Co, 1976, p 81. 13. Knox P, Crooks S, Rimer CS: Role of fibronectin in the migration of fibroblasts into plasma clots . J Cell Biol 1986; 102:2318-2323.Crossref 14. Clark RA, Lanigan JM, Dellapella P, et al: Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization . J Invest Dermatol 1983;799:264-269. 15. Donaldson DJ, Malhan JT: Fibrinogen and fibronectin as substrates for epidermal cell migration during wound closure . J Cell Sci 1983;62:117-127. 16. Baur PS, Parks DH: The myofibroblast anchoring strand: The fibronectin connection in wound healing and the possible loci of collagen fibril assembly . J Trauma 1983;23:853-862.Crossref 17. McDonagh J: Fibronectin: A molecular glue . Arch Pathol Lab Med 1981;105:393-396. 18. Mosher DF: Physiology of fibronectin . Ann Rev Med 1984;35:561-575.Crossref 19. Giancotti FG, Comoglio PM, Tarone G: Fibronectin-plasma membrane interaction in the adhesion of hemopoietic cells . J Cell Biol 1986;103:429-437.Crossref 20. Hosein B, Bianco C: Monocyte receptors for fibronectin characterized by monoclonal antibody that interferes with receptor activity . J Exp Med 1985;162:157-170.Crossref 21. Reese AC, Doran JE, Callaway BD, et al: Sequestration of fibronectin at the site of an injury . Adv Shock Res 1982;8:119-127. 22. Lanser ME, Saba TM, Scovill W: Opsonic glycoprotein (plasma fibronectin) levels after burn injury: Relationship to extent of burn and development of sepsis . Ann Surg 1980;192:776-789.Crossref 23. Dionyssiou-Asteriou A, Karavassilis J: Level of plasma fibronectin in patients with vascular disease . Clin Chim Acta 1985;152:79-83.Crossref 24. Richards WO, Scovill WA, Shin B: Opsonic fibronectin deficiency in patients with intra-abdominal infection . Surgery 1983;94:210-217. 25. Lanser ME, Saba TM, Scovill W: Opsonic glycoprotein (plasma fibronectin) levels after burn injury: Relationship to extent of burn and development of sepsis . Ann Surg 1980;192:776-789.Crossref 26. Saba TM, DiLuzio NA: Reticuloendothelial blockade and recovery as a function of opsonic activity . Am J Physiol 1969;216:197-205. 27. Lundsgaard-Hansen P, Doran JE, Rubli E, et al: Purified fibronectin administration to patients with severe abdominal infections: A controlled study . Ann Surg 1985;202:745-759.Crossref 28. Saba TM, Blumenstock FA, Bernard H: Cryoprecipitate reversal of opsonic alpha-2-surface binding glycoprotein deficiencies in septic surgical trauma patients . Science 1978;201:622-624.Crossref 29. Doran JE, Mansberger AR, Reese AC: Cold insoluble globulin-enhanced phagocytosis of gelatinized targets by macrophage monolayers . J Reticuloendothel Soc 1980;27:471-483. 30. Ruoslahti E, Hayman EG, Piershbacher M, et al: Fibronectin: Purification, immunochemical properties, and biological activities . Methods Enzymol 1982;82:803-831. 31. Kono I, Matsumoto Y, Kono K, et al: Beneficial effects of topical fibronectin in patients with keratoconjunctivitis sicca of Sjogren's syndrome . J Rheumatol 1985;12:487-489. 32. Nishida T, Nakagawa S, Nishibayashi C, et al: Fibronectin enhancement of corneal epithelial wound healing of rabbits in vivo . Arch Ophthalmol 1984;102:455-457.Crossref 33. Rubin LF: Toxicologic update of dimethyl sulfoxide . Ann NY Acad Sci 1983;411:6-10.Crossref 34. Jacob SW, Herschler RJ, Rosenbaum EE: Dimethyl sulfoxide (DMSO): Laboratory and clinical evaluation . J Am Vet Med Assoc 1965;147:1350-1359. 35. Shirley SW, Stewart BH, Mirelman A: Dimethyl sulfoxide in treatment of inflammatory genitourinary disorders . Urology 1978;11:215-220.Crossref 36. Rao CV: Differential effects of detergents and dimethyl sulfoxide on membrane prostaglandin E and F2a receptors . Life Sci 1977;20:2013-2022.Crossref 37. Wood DC, Wood J: Pharmacologic and biochemical considerations of dimethylsulfoxide . Ann NY Acad Sci 1975:243:7-19.Crossref 38. Goldblum OM, Alvarez OM, Mertz MM, et al: Dimethyl sulfoxide (DMSO) does not affect epidermal wound healing . Proc Soc Exp Biol Med 1983;172:301-307.Crossref 39. Rollins BHJ, Cathcart MK, Culp LA: Fibronectin proteoglycan binding as the molecular basis for fibroblast adhesion to extracellular matrices , in Howowitz MI (ed): The Glycoconjugates . Orlando, Fla, Academic Press Inc, 1982, vol 3, pp 289-329. 40. Grinnell F, Feld M, Minter D: Fibroblast adhesion to fibrinogen and fibrin substrata: Requirement of cold-insoluble globulin (plasma fibronectin) . Cell 1980;19:517-528.Crossref 41. Peacock EE Jr: Wound Repair , ed 3. Philadelphia, WB Saunders Co, 1984, pp 38-55.

Journal

Archives of DermatologyAmerican Medical Association

Published: Feb 1, 1988

References