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Fate of Cells in the Corneal Graft

Fate of Cells in the Corneal Graft Abstract The aim of the corneal transplant in man is to maintain corneal transparency. Even under the best conditions a proportion of the grafts that initially "take" suffer from the clinical problem of "graft sickness" with late clouding. These grafts are the homoplastic type, and evidence is accumulating which indicates that the homograft rejection phenomenon in the cornea is an immune response.1,2 Thus, the clouding in an otherwise successful graft would result from a reaction of the host to the antigen of the donor tissue. An observation made by Merwin and Hill,3 in working with subcutaneous homografts in mice, was that only those grafts which did not vascularize survive. This led to further studies and observations that homografts in cell-impervious millipore membranes survived in normal and in tissue-immunized mice.4,5 From this and other experiments, it was concluded that in this example the antibodies cytotoxic to the homografts were References 1. Each cubic centimeter contains polymixin B sulfate 5,000 units, neomycin sulfate 2.5 mg., gramicidin 0.025 mg. Manufactured by Burroughs Wellcome & Co., Inc., 1 Scarsdale Road, Tuckahoe 7, N.Y. 2. DeVoe, A. G.: Keratoplasty: Past, Present, and Future , Arch. Ophthal. 66:652, 1961.Crossref 3. Dohlman, C. H., in Duke-Elder, S., and Perkins, E. S.: Transparency of the Cornea , Oxford, Blackwell Scientific Publications, 1960, p. 171. 4. Merwin, R. M., and Hill, E. L.: Fate of Vascularized and Nonvascularized Subcutaneous Homografts in Mice , J. Nat. Cancer Inst. 14:819, 1954. 5. Weaver, J. M.; Algire, G. H., and Prehn, R. T.: The Growth of Cells in Vivo in Diffusion Chambers , J. Nat. Cancer Inst. 15:1737, 1955. 6. Algire, G. H.; Weaver, J. M., and Prehn, R. T.: Growth of Cells in Vivo in Diffusion Chambers: I. Survival of Homografts in Immunized Mice , J. Nat. Cancer Inst. 15:493, 1954. 7. Espiritu, R. B.; Kara, G. B., and Tabowitz, D.: Studies on the Healing of Corneal Grafts: I. The Fate of Mucopolysaccharides as Determined by S25 Incorporation and Autoradiography , Amer. J. Ophthal. 51:1281, 1961. 8. Basu, P. K.; Miller, I., and Ormsby, H. L.: Sex Chromatin as a Biological Marker: In the Study of the Fate of Corneal Transplants , Amer. J. Ophthal. 49:513, 1960. 9. Espiritu, R. B.; Kara, G. B., and Tabowitz, D.: Studies on the Healing of Corneal Grafts: II. Fate of the Endothelial Cells of the Graft as Determined by Sex Chromatin Studies , Amer. J. Ophthal. 52:91, 1961. 10. Hanna, C., and O'Brien, J. E.: Thymidine Tritium Labeling of the Cellular Elements of the Corneal Stroma , Arch. Ophthal. 66:362, 1961.Crossref 11. Hanna, C., and O'Brien, J. E.: Cell Production and Migration in the Epithelial Layer of the Cornea , Arch. Ophthal. 64:536, 1960.Crossref 12. Bito, L. Z., and Harding, C. V.: Tritium Retention by Corneal Endothelium After Incorporation of H3-Thymidine , Arch. Ophthal. 65:553, 1961.Crossref 13. Abrahamson, I. A., Jr.; Brumm, L. P., and Abrahamson, I. A.: A Simplified Technique for Corneal Transplantation , Amer. J. Ophthal. 41:79, 1956. 14. Mills, N. L., and Donn, A.: Incorporation of Tritium-Labeled Thymidine by Rabbit Corneal Endothelium , Arch. Ophthal. 64:443, 1960.Crossref 15. Polack, F. M.: Personal communication to the author, 1962. 16. Hanna, C.: Fate of Cellular Elements Following Keratoplasty , Abstract, Fed. Proc. 21:38, 1962. 17. Polack, F. M., and Smelser, G. K.: Survival of Isotopically Labeled Cells in the Corneal Homograft , Anat. Rec. 142:268, 1962 18. Abstract; Polack, F. M., and Smelser, G. K.: The Persistence of Isotopically Labeled Cells in the Corneal Grafts, Proc. Soc. Exp. Biol. Med., to be published, 1962. 19. Polack, F. M.: Personal communication to the author. 20. Billingham, R. E., and Boswell, T.: Studies on the Problem of Corneal Homografts , Proc. Roy. Soc. [B] 151:392, 1953.Crossref 21. Maumenee, A. E.: The Influence of Donor Recipient Sensitization on Corneal Grafts , Amer. J. Ophthal. 34:142 (May, (Pt. 2) ), 1951. 22. Longmire, W. P.; Cannon, J. A., and Weber, R. A., in Preservation and Transplantation of Normal Tissues, Ciba Foundation Symposium , London, 1954, p. 23. 23. Katzin, H. M.: The Preservation of Corneal Tissue by Freezing and Dehydrating , Amer. J. Ophthal. 30:1128, 1947. 24. Geeraets, W. J.; Lieb, W. A.; Chan, G., and Guerry, D., III: Immunochemical Analysis of Experimental Corneal Transplants , Amer. J. Ophthal. 49:740, 1960. 25. Medawar, P. B., in Peer, L. A.: Transplantation of Tissues , Baltimore, The Williams & Wilkins Company, 1959, Vol. 2, p. 56. 26. Woodruff, M. F. A.: The "Critical Period" of Homografts , Transplant. Bull. 1:221, 1954. 27. Cannon, J. A.: The Question of Host Adaptation Versus Graft Adaptation in Successful Homografts , Transplant. Bull. 4:22, 1957. 28. Duke-Elder, S.: The Problem of Homoplastic Grafting as Applied to the Cornea , J. Roy. Coll. Surg. Edinburgh 1:187, 1955. 29. Medawar, P. B.: The Behavior and Fate of Skin Homografts in Rabbits , J. Anat. 78:176, 1944. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Fate of Cells in the Corneal Graft

Archives of Ophthalmology , Volume 68 (6) – Dec 1, 1962

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Publisher
American Medical Association
Copyright
Copyright © 1962 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1962.00960030814016
Publisher site
See Article on Publisher Site

Abstract

Abstract The aim of the corneal transplant in man is to maintain corneal transparency. Even under the best conditions a proportion of the grafts that initially "take" suffer from the clinical problem of "graft sickness" with late clouding. These grafts are the homoplastic type, and evidence is accumulating which indicates that the homograft rejection phenomenon in the cornea is an immune response.1,2 Thus, the clouding in an otherwise successful graft would result from a reaction of the host to the antigen of the donor tissue. An observation made by Merwin and Hill,3 in working with subcutaneous homografts in mice, was that only those grafts which did not vascularize survive. This led to further studies and observations that homografts in cell-impervious millipore membranes survived in normal and in tissue-immunized mice.4,5 From this and other experiments, it was concluded that in this example the antibodies cytotoxic to the homografts were References 1. Each cubic centimeter contains polymixin B sulfate 5,000 units, neomycin sulfate 2.5 mg., gramicidin 0.025 mg. Manufactured by Burroughs Wellcome & Co., Inc., 1 Scarsdale Road, Tuckahoe 7, N.Y. 2. DeVoe, A. G.: Keratoplasty: Past, Present, and Future , Arch. Ophthal. 66:652, 1961.Crossref 3. Dohlman, C. H., in Duke-Elder, S., and Perkins, E. S.: Transparency of the Cornea , Oxford, Blackwell Scientific Publications, 1960, p. 171. 4. Merwin, R. M., and Hill, E. L.: Fate of Vascularized and Nonvascularized Subcutaneous Homografts in Mice , J. Nat. Cancer Inst. 14:819, 1954. 5. Weaver, J. M.; Algire, G. H., and Prehn, R. T.: The Growth of Cells in Vivo in Diffusion Chambers , J. Nat. Cancer Inst. 15:1737, 1955. 6. Algire, G. H.; Weaver, J. M., and Prehn, R. T.: Growth of Cells in Vivo in Diffusion Chambers: I. Survival of Homografts in Immunized Mice , J. Nat. Cancer Inst. 15:493, 1954. 7. Espiritu, R. B.; Kara, G. B., and Tabowitz, D.: Studies on the Healing of Corneal Grafts: I. The Fate of Mucopolysaccharides as Determined by S25 Incorporation and Autoradiography , Amer. J. Ophthal. 51:1281, 1961. 8. Basu, P. K.; Miller, I., and Ormsby, H. L.: Sex Chromatin as a Biological Marker: In the Study of the Fate of Corneal Transplants , Amer. J. Ophthal. 49:513, 1960. 9. Espiritu, R. B.; Kara, G. B., and Tabowitz, D.: Studies on the Healing of Corneal Grafts: II. Fate of the Endothelial Cells of the Graft as Determined by Sex Chromatin Studies , Amer. J. Ophthal. 52:91, 1961. 10. Hanna, C., and O'Brien, J. E.: Thymidine Tritium Labeling of the Cellular Elements of the Corneal Stroma , Arch. Ophthal. 66:362, 1961.Crossref 11. Hanna, C., and O'Brien, J. E.: Cell Production and Migration in the Epithelial Layer of the Cornea , Arch. Ophthal. 64:536, 1960.Crossref 12. Bito, L. Z., and Harding, C. V.: Tritium Retention by Corneal Endothelium After Incorporation of H3-Thymidine , Arch. Ophthal. 65:553, 1961.Crossref 13. Abrahamson, I. A., Jr.; Brumm, L. P., and Abrahamson, I. A.: A Simplified Technique for Corneal Transplantation , Amer. J. Ophthal. 41:79, 1956. 14. Mills, N. L., and Donn, A.: Incorporation of Tritium-Labeled Thymidine by Rabbit Corneal Endothelium , Arch. Ophthal. 64:443, 1960.Crossref 15. Polack, F. M.: Personal communication to the author, 1962. 16. Hanna, C.: Fate of Cellular Elements Following Keratoplasty , Abstract, Fed. Proc. 21:38, 1962. 17. Polack, F. M., and Smelser, G. K.: Survival of Isotopically Labeled Cells in the Corneal Homograft , Anat. Rec. 142:268, 1962 18. Abstract; Polack, F. M., and Smelser, G. K.: The Persistence of Isotopically Labeled Cells in the Corneal Grafts, Proc. Soc. Exp. Biol. Med., to be published, 1962. 19. Polack, F. M.: Personal communication to the author. 20. Billingham, R. E., and Boswell, T.: Studies on the Problem of Corneal Homografts , Proc. Roy. Soc. [B] 151:392, 1953.Crossref 21. Maumenee, A. E.: The Influence of Donor Recipient Sensitization on Corneal Grafts , Amer. J. Ophthal. 34:142 (May, (Pt. 2) ), 1951. 22. Longmire, W. P.; Cannon, J. A., and Weber, R. A., in Preservation and Transplantation of Normal Tissues, Ciba Foundation Symposium , London, 1954, p. 23. 23. Katzin, H. M.: The Preservation of Corneal Tissue by Freezing and Dehydrating , Amer. J. Ophthal. 30:1128, 1947. 24. Geeraets, W. J.; Lieb, W. A.; Chan, G., and Guerry, D., III: Immunochemical Analysis of Experimental Corneal Transplants , Amer. J. Ophthal. 49:740, 1960. 25. Medawar, P. B., in Peer, L. A.: Transplantation of Tissues , Baltimore, The Williams & Wilkins Company, 1959, Vol. 2, p. 56. 26. Woodruff, M. F. A.: The "Critical Period" of Homografts , Transplant. Bull. 1:221, 1954. 27. Cannon, J. A.: The Question of Host Adaptation Versus Graft Adaptation in Successful Homografts , Transplant. Bull. 4:22, 1957. 28. Duke-Elder, S.: The Problem of Homoplastic Grafting as Applied to the Cornea , J. Roy. Coll. Surg. Edinburgh 1:187, 1955. 29. Medawar, P. B.: The Behavior and Fate of Skin Homografts in Rabbits , J. Anat. 78:176, 1944.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Dec 1, 1962

References