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Experimental Retinal Detachment: VI. The Permeability of the Blood-Retinal Barrier

Experimental Retinal Detachment: VI. The Permeability of the Blood-Retinal Barrier Abstract • Fluorophotometry was used to study the permeability of the blood-retinal barrier in six monkeys with stable rhegmatogenous retinal detachments. Fluorescein transport was inhibited by probenecid. The rate of fluorescein disappearance (KvVv) was determined following intravitreal injection. In a separate experiment the equilibrium vitreous-plasma concentration ratio (Cv/Cp) was determined following intraperitoneal administration. Expressed in equivalent volumes of vitreous, the rate of fluorescein diffusion across the blood-retinal barrier (K′vVvCv/ Cp) was 0.29 μL/min in control eyes and 0.73 μL/min in detached eyes. The rate of fluid movement across the blood-retinal barrier (K′vVv) [1 - (Cv/Cp)] was 2.89 μL/min in control eyes and 6.38 μL/min in detached eyes. Posterior movement of fluid contributes to retinal apposition under normal conditions and accounts for the rapid resolution of retinal detachment following closure of the retinal hole. References 1. Arruga H: Etioiogia y patogenia del desprendamientio de la retina, vol 2, pp 110-114. Program of the 14th International Congress of Ophthalmology, Madrid, April 1933. 2. Dobbie JG: A study of the intraocular fluid dynamics in retinal detachment . Arch Ophthalmol 1963;69:159-164.Crossref 3. Regan CDJ, Rousseau AP: The intraocular dynamics of eyes with retinal detachment . Am J Ophthalmol 1966;61:696-702. 4. Hayreh SS: Posterior drainage of the intraocular fluid from the vitreous . Exp Eye Res 1966;5:123-144.Crossref 5. Foulds WS: Experimental detachment of the retina and its effect on intraocular fluid dynamics . Mod Probl Ophthalmol 1969;8:51-63. 6. Kaufman PL, Podos SM: Subretinal fluid in primary rhegmatogenous retinal detachment . Surv Ophthalmol 1973;18:100-116. 7. Foulds WS: Aetiology of retinal detachment . Trans Ophthalmol Soc UK 1975;95:118-127. 8. Foulds WS: Clinical significance of transscleral fluid transfer . Trans Ophthalmol Soc UK 1976;96:290-308. 9. Foulds WS: The intraocular fluids . Trans Ophthalmol Soc UK 1977;97:543-546. 10. Custodis E: Beobachtungen bei der diathermischen Behandlung der Netzhautablösung und ein Hinweis zur Therapie der Amotio Retinae . Ber Zusammenkunft Dtsch Ophthalmol Ges 1952;227:57-67. 11. Fatt I, Shantinath K: Flow conductivity of retina and its role in retinal adhesion . Exp Eye Res 1971;12:218-226.Crossref 12. Pederson JE, Cantrill HL, Cameron JD: Experimental retinal detachment: II. Role of the vitreous . Arch Ophthalmol 1982;100:1155-1159.Crossref 13. Cantrill HL, Pederson JE: Experimental retinal detachment: III. Vitreous fluorophotometry . Arch Ophthalmol 1982;100:1810-1813.Crossref 14. Brubaker RF, Coakes RL: Use of a xenon flash tube as the excitation source in a new slit lamp fluorophotometer . Am J Ophthalmol 1978; 86:474-484. 15. Maurice DM: Protein dynamics in the eye studied with labelled protein . Am J Ophthalmol 1959;47:361-368. 16. Taylor E, Jennings A: Calculation of total retinal area . Br J Ophthalmol 1971;55:262-265.Crossref 17. Cunha-Vaz: The blood ocular barriers . Surv Ophthalmol 1979;23:279-296.Crossref 18. Peyman GA, Spitznas M, Straatsma BR: Chorioretinal diffusion of peroxidase before and after photocoagulation . Invest Ophthalmol Vis Sci 1971;10:489-495. 19. Peyman GA, Spitznas M, Straatsma BR: Peroxidase diffusion in the normal and photocoagulated retina . Invest Ophthalmol Vis Sci 1971; 10:181-189. 20. Stone RA, Wilson CM: Fluorescein transport in the anterior uvea . Invest Ophthalmol Vis Sci 1982;22:303-309. 21. Cunha-Vaz JG, Maurice DM: The active transport of fluorescein by the retinal vessels and the retina . J Physiol 1967;191:467-486. 22. Zweig K, Cunha-Vaz JG, Payman G, et al: Effect of argon laser photocoagulation on fluorescein transport across the blood-retinal barrier . Exp Eye Res 1981;32:323-329.Crossref 23. Salminen L, Fischer W, Jantti V, et al: Rate of loss of intravitreally injected fluorescein and EDTA in the rabbit eye . Ophthal Res 1981; 13:60-64.Crossref 24. Palestine AG, Brubaker RF: Pharmacokinetics of fluorescein in the vitreous . Invest Ophthalmol Vis Sci 1981;21:542-549. 25. Zeimer RC, Blair NT, Cunha-Vaz JG, et al: In vivo evaluation of the inward and outward permeability of the normal human blood-retinal barrier , abstracted. Invest Ophthalmol Vis Sci 1983;24( (suppl) ):210. 26. Frambach DA, Marmor MF: The rate and route of fluid resorption from the subretinal space of the rabbit . Invest Ophthalmol Vis Sci 1982;22:292-302. 27. Miller SF: Cyclic AMP-modulated transport in retinal pigment epithelium , abstracted. Invest Ophthalmol Vis Sci 1982;22( (suppl) ):13. 28. Brown JA, Zadunaisky JA: Ion coupled fluid movements across the bullfrog retinal pigment epithelium , abstracted. Invest Ophthalmol Vis Sci 1982;22( (suppl) ):102. 29. Pederson JD, Cantrill HL: Experimental retinal detachment: V. Fluid movement through the retinal hole . Arch Ophthalmol 1984;102:136-139.Crossref 30. Pederson JE: Experimental retinal detachment: IV. Aqueous humor dynamics in rhegmatogenous detachments . Arch Ophthalmol 1982;100:1814-1816.Crossref 31. Kroll AJ, Machemer R: Experimental detachment and reattachment: II. Electron microscopy . Mod Probl Ophthalmol 1969;8:91-105. 32. Pederson JE, MacLellan HM: Experimental retinal detachment: I. Effect of subretinal fluid composition on reabsorption rate and intraocular pressure . Arch Ophthalmol 1982; 100:1150-1154.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Experimental Retinal Detachment: VI. The Permeability of the Blood-Retinal Barrier

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Publisher
American Medical Association
Copyright
Copyright © 1984 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1984.01040030595029
Publisher site
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Abstract

Abstract • Fluorophotometry was used to study the permeability of the blood-retinal barrier in six monkeys with stable rhegmatogenous retinal detachments. Fluorescein transport was inhibited by probenecid. The rate of fluorescein disappearance (KvVv) was determined following intravitreal injection. In a separate experiment the equilibrium vitreous-plasma concentration ratio (Cv/Cp) was determined following intraperitoneal administration. Expressed in equivalent volumes of vitreous, the rate of fluorescein diffusion across the blood-retinal barrier (K′vVvCv/ Cp) was 0.29 μL/min in control eyes and 0.73 μL/min in detached eyes. The rate of fluid movement across the blood-retinal barrier (K′vVv) [1 - (Cv/Cp)] was 2.89 μL/min in control eyes and 6.38 μL/min in detached eyes. Posterior movement of fluid contributes to retinal apposition under normal conditions and accounts for the rapid resolution of retinal detachment following closure of the retinal hole. References 1. Arruga H: Etioiogia y patogenia del desprendamientio de la retina, vol 2, pp 110-114. Program of the 14th International Congress of Ophthalmology, Madrid, April 1933. 2. Dobbie JG: A study of the intraocular fluid dynamics in retinal detachment . Arch Ophthalmol 1963;69:159-164.Crossref 3. Regan CDJ, Rousseau AP: The intraocular dynamics of eyes with retinal detachment . Am J Ophthalmol 1966;61:696-702. 4. Hayreh SS: Posterior drainage of the intraocular fluid from the vitreous . Exp Eye Res 1966;5:123-144.Crossref 5. Foulds WS: Experimental detachment of the retina and its effect on intraocular fluid dynamics . Mod Probl Ophthalmol 1969;8:51-63. 6. Kaufman PL, Podos SM: Subretinal fluid in primary rhegmatogenous retinal detachment . Surv Ophthalmol 1973;18:100-116. 7. Foulds WS: Aetiology of retinal detachment . Trans Ophthalmol Soc UK 1975;95:118-127. 8. Foulds WS: Clinical significance of transscleral fluid transfer . Trans Ophthalmol Soc UK 1976;96:290-308. 9. Foulds WS: The intraocular fluids . Trans Ophthalmol Soc UK 1977;97:543-546. 10. Custodis E: Beobachtungen bei der diathermischen Behandlung der Netzhautablösung und ein Hinweis zur Therapie der Amotio Retinae . Ber Zusammenkunft Dtsch Ophthalmol Ges 1952;227:57-67. 11. Fatt I, Shantinath K: Flow conductivity of retina and its role in retinal adhesion . Exp Eye Res 1971;12:218-226.Crossref 12. Pederson JE, Cantrill HL, Cameron JD: Experimental retinal detachment: II. Role of the vitreous . Arch Ophthalmol 1982;100:1155-1159.Crossref 13. Cantrill HL, Pederson JE: Experimental retinal detachment: III. Vitreous fluorophotometry . Arch Ophthalmol 1982;100:1810-1813.Crossref 14. Brubaker RF, Coakes RL: Use of a xenon flash tube as the excitation source in a new slit lamp fluorophotometer . Am J Ophthalmol 1978; 86:474-484. 15. Maurice DM: Protein dynamics in the eye studied with labelled protein . Am J Ophthalmol 1959;47:361-368. 16. Taylor E, Jennings A: Calculation of total retinal area . Br J Ophthalmol 1971;55:262-265.Crossref 17. Cunha-Vaz: The blood ocular barriers . Surv Ophthalmol 1979;23:279-296.Crossref 18. Peyman GA, Spitznas M, Straatsma BR: Chorioretinal diffusion of peroxidase before and after photocoagulation . Invest Ophthalmol Vis Sci 1971;10:489-495. 19. Peyman GA, Spitznas M, Straatsma BR: Peroxidase diffusion in the normal and photocoagulated retina . Invest Ophthalmol Vis Sci 1971; 10:181-189. 20. Stone RA, Wilson CM: Fluorescein transport in the anterior uvea . Invest Ophthalmol Vis Sci 1982;22:303-309. 21. Cunha-Vaz JG, Maurice DM: The active transport of fluorescein by the retinal vessels and the retina . J Physiol 1967;191:467-486. 22. Zweig K, Cunha-Vaz JG, Payman G, et al: Effect of argon laser photocoagulation on fluorescein transport across the blood-retinal barrier . Exp Eye Res 1981;32:323-329.Crossref 23. Salminen L, Fischer W, Jantti V, et al: Rate of loss of intravitreally injected fluorescein and EDTA in the rabbit eye . Ophthal Res 1981; 13:60-64.Crossref 24. Palestine AG, Brubaker RF: Pharmacokinetics of fluorescein in the vitreous . Invest Ophthalmol Vis Sci 1981;21:542-549. 25. Zeimer RC, Blair NT, Cunha-Vaz JG, et al: In vivo evaluation of the inward and outward permeability of the normal human blood-retinal barrier , abstracted. Invest Ophthalmol Vis Sci 1983;24( (suppl) ):210. 26. Frambach DA, Marmor MF: The rate and route of fluid resorption from the subretinal space of the rabbit . Invest Ophthalmol Vis Sci 1982;22:292-302. 27. Miller SF: Cyclic AMP-modulated transport in retinal pigment epithelium , abstracted. Invest Ophthalmol Vis Sci 1982;22( (suppl) ):13. 28. Brown JA, Zadunaisky JA: Ion coupled fluid movements across the bullfrog retinal pigment epithelium , abstracted. Invest Ophthalmol Vis Sci 1982;22( (suppl) ):102. 29. Pederson JD, Cantrill HL: Experimental retinal detachment: V. Fluid movement through the retinal hole . Arch Ophthalmol 1984;102:136-139.Crossref 30. Pederson JE: Experimental retinal detachment: IV. Aqueous humor dynamics in rhegmatogenous detachments . Arch Ophthalmol 1982;100:1814-1816.Crossref 31. Kroll AJ, Machemer R: Experimental detachment and reattachment: II. Electron microscopy . Mod Probl Ophthalmol 1969;8:91-105. 32. Pederson JE, MacLellan HM: Experimental retinal detachment: I. Effect of subretinal fluid composition on reabsorption rate and intraocular pressure . Arch Ophthalmol 1982; 100:1150-1154.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: May 1, 1984

References