Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Excitation Transfer in Bone: Deleterious Effects of Cobalt 60 Radiation-Sterilization of Bank Bone

Excitation Transfer in Bone: Deleterious Effects of Cobalt 60 Radiation-Sterilization of Bank Bone Abstract In a bone alloimplant in muscle, the structure of the organic matrix evocates or promotes a morphogenetic response from migratory mesenchymal cells. Radiation sterilization destroys the morphogenetic property at a much lower dose of cobalt 60 in undemineralized than in the demineralized bone. Damage to the organic matrix is heightened by excitation transfer from the radiated bone mineral to the matrix proteins. The mineral having an apatite structure also has a relatively high ionization potential and generates deleterious free radicals, which convert the matrix collagen to gelatin. Radiolysis prevents neither the undesirably delayed hypersensitivity response nor the degradation of the bone morphogenetic property by endogenous protease. These observations account for the failure of freeze-dried and radiation-sterilized bone to promote repair of large bone defects in adult patients. References 1. Buring K, Urist MR: Effects of ionizing radiation on the bone induction principle in matrix of bone implants . Clin Orthop 55:225-234, 1967. 2. Buring K: Ionizing radiation for sterilization of bone , in Sterilization and Preservation of Biological Tissues by Ionizing Radiation . Vienna, International Atomic Energy Agency, 1970, pp 71-78. 3. Santin LN: Sterilization of bone tissue with gamma rays of cobalt 60 . Radiobiologiia 3:621-625, 1963. 4. Volkov M, Bizer V: Homeotransplantation of bone tissue in children . Moscow, MIR Publishers, 1972, p 232. 5. Bailey AJ, Tromas WJ: Effects of ionizing radiation on the ultrastructure of collagen fibrils . Radiat Res 23:145-155, 1964.Crossref 6. Czerniewski M, et al: Bone tissue sterilization using reactor fuel gamma radiation . Nukleonika 10:19-28, 1973. 7. Wright KA, Trump JG: Cooperative studies in the use of ionizing radiation for sterilization and preservation of biological tissues , in Sterilization and Preservation of Biological Tissues by Ionizing Radiation . Vienna, International Atomic Energy Agency, 1970, pp 107-118. 8. Ostrowski K: Current problems of tissue banking . Transplant Proc 1:126-131, 1969. 9. Ostrowski K, Moskalewski S, Komender J: Teoretyczne aspekty sterylizacji promieniowaniem jonizujacym przeszczepow dla potrzeb . Klin Chir Narzadow Ruchia Orthop Pol 33:311-320, 1968. 10. Cohen J: Cathode ray sterilization of bone grafts . Arch Surg 71:784-789, 1955.Crossref 11. Bassett CAL, Packard AG Jr: A clinical assay of cathode ray sterilized bone grafts . Acta Orthop Scand 28:198-211, 1959.Crossref 12. Marmour L: Bone banks: A new concept in procurement and storage for homologous grafts . Calif Med 92:407-408, 1960. 13. Turner TC, Bassett CB, Sawyer PN: Sterilization of preserved bone grafts by high voltage cathode-irradiation . J Bone Joint Surg 38-A:862-884, 1956. 14. Urist MR: Bone: Formation by autoinduction . Science 150:893-899, 1965.Crossref 15. Urist MR: The substratum for bone morphogenesis . Dev Biol , (suppl 4) , pp 125-163, 1970. 16. Urist MR, et al: Quantitation of new bone formation in intramuscular implants of bone matrix in rabbits . Clin Orthop 68:279-293, 1970.Crossref 17. Urist MR, et al: The bone induction principle . Clin Orthop 53:243-283, 1967.Crossref 18. Urist MR, Strates BS: Bone morphogenetic protein . J Dent Res 50:1392-1406, 1971.Crossref 19. Van de Putte KA, Urist MR: Osteogenesis in the interior of intramuscular implants of decalcified bone matrix . Clin Orthop 43:257-270, 1966.Crossref 20. Urist MR, et al: Bone morphogenesis in implants of insoluble bone gelatin . Proc Natl Acad Sci USA 70:3511-3515, 1973.Crossref 21. Urist MR, et al: Bone morphogenesis in implants of residues of radioisotope labelled bone matrix. Calcif Tissue Res, to be published. 22. Urist MR, Iwata H: Preservation and biodegradation of the morphogenetic property of bone matrix . J Theor Biol 38:155-167, 1973.Crossref 23. Urist MR: Enzymes in bone morphogenesis: Endogenous enzymic degradation of the morphogenetic property in bone in solutions buffered by ethylenediaminetetraacetic acid (EDTA) , in Hard Tissue Growth, Repair and Remineralization, Ciba Symposium 11 . Amsterdam, Associated Scientific Publication, 1973, pp 143-160. 24. Urist MR, et al: Observations implicating an extracellular enzymic mechanism of control of bone morphogenesis . J Histochem Cytochem 22:88-103, 1974.Crossref 25. Mikulski AJ, Urist MR: Control of bone morphogenesis by a matrix glycopeptide and protease , abstracted. J Dent Res 53 (special issue):138, 1974.Crossref 26. Iwata H, Urist MR: Protein polysaccharide of bone morphogenetic matrix . Clin Orthop 87:257-274, 1972.Crossref 27. Christensen EA: Bacterial evaluation of sterilization procedures . Ann Pharm Fr 5:3-12, 1966. 28. Ostrowski K: Current problems of tissue banking . Transplant Proc 1:126-131, 1969. 29. Houben JL: Free radicals produced by ionizing radiation in bone and its constituents . Int J Radiat Biol 20:373-389, 1971.Crossref 30. Termine JD, Pullman I, Posner AS: Electron spin resonance study of irradiated bone and its constituents . Arch Biochem Biophys 122:318-330, 1967.Crossref 31. Ostrowski K, et al: Application of electron spin resonance in research on mineralized tissues . Clin Orthop 97:213-224, 1973.Crossref 32. Becker RO: Electron paramagnetic resonance in non-irradiated bone . Nature 199:1304-1305, 1963.Crossref 33. Gordy W, Ard WB, Shields H: Microwave spectroscopy of biological substances: I. Paramagnetic resonance of X-irradiated amino acids and proteins . Proc Natl Acad Sci USA 41:983-1004, 1955.Crossref 34. Fischer BV, et al: Radiation damage in calcium phosphates and collagen: An interpretation of ESR spectra . Radiat Res 46:229-235, 1971.Crossref 35. Stachowicz W, et al: On free radicals evoked by radiosterilization in preserved bone grafts , in Sterilization and Preservation of Biological Tissues by Ionizing Radiation . Vienna, International Atomic Energy Agency, 1970, pp 15-27. 36. Slager UT, Zucker MJ, Reilly EB: The persistance of electron spin resonance in bone grafts sterilized by ionizing radiation . Radiat Res 22:556-563, 1964.Crossref 37. Stachowicz W, et al: ESR study of bone tissue sterilized by gamma irradiation . Nukleonika 15:131-142, 1970. 38. Stachowicz W, et al: Deproteinized bone powder as a dosimeter for radiosterilization of biostatic grafts . Nukleonika 17:425-430, 1972. 39. Swartz HM: Long-lived electron spin resonance in rats irradiated at room temperature . Radiat Res 24:579-586, 1965.Crossref 40. Bonar LC, Glimcher MJ: Thermal denaturation of mineralized and demineralized bone collagens . J Ultrastruct Res 32:545-551, 1970.Crossref 41. Slager UT, Zucker MJ: The occurrence of electron spin resonance signals in bone grafts sterilized with high voltage electron beams . Transplant Bull 30:536-563, 1962.Crossref 42. Kausch W: Ueber knochenimplantation . Verh Dtsch Ges Chir 35:179-188, 1906. 43. Kausch W: Zur frage der freien transplantation toten knochens . Zentralbl Chir 36:1379-1381, 1909. 44. Kausch W: Ueber knochenersatz: Beitrage zur transplantation toten knochens . Beitr Klin Chir 68:670-716, 1910. 45. Gallie WE, Robertson DE: The transplantation of bone . JAMA 70:1134-1140, 1919.Crossref 46. Leriche R: Sur les greffes d'os mort et sur les greffes onoplastiques et heteroplastiques . Mem Acad Chir 76:389-410, 1950. 47. Rocher HL: Dead bone grafts in orthopaedic surgery . J Bone Joint Surg 35-B:328-329, 1953. 48. Burwell RG: The fate of bone grafts , in Apley AG (ed): Recent Advances in Orthopaedics . Baltimore, Williams & Wilkins Co, 1969, pp 115-207. 49. Lance EM: Bone and cartilage , in Najarian JS, Simmons RL (eds): Transplantation . Philadelphia, Lea & Febiger, 1972, pp 655-697. 50. Chalmers J: Transplantation immunity in bone homografting . J Bone Joint Surg 41-B:160-179, 1959. 51. Heiple KG, Chase SN, Herndon CH: A comparative study of the healing process following different types of bone transplantation . J Bone Joint Surg 45-A:1593-1616, 1963. 52. Bonfiglio M, Jeter WS: Immunological responses to bone . Clin Orthop 87:19-27, 1972. 53. Urist MR: Osteoinduction in undemineralized bone implants modified by chemical inhibitors of endogenous matrix enzymes . Clin Orthop 87:132-137, 1972.Crossref 54. Immaliev AS: The preparation preservation and transplantation of articular bone ends , in Apley AG (ed): Recent Advances in Orthopaedics . Baltimore, Williams & Wilkins Co, 1969, pp 209-263. 55. Okada S: Radiation Biochemistry . New York, Academic Press Inc, 1970. 56. Shore JS, Tobert BM, Sauerlich HE: The effects of ionizing radiation on trypsin: I. Losses of esterase and protease activities of trypsin and trypsin inhibitor compound . Arch Biochem Biophys 102:125-131, 1963.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Excitation Transfer in Bone: Deleterious Effects of Cobalt 60 Radiation-Sterilization of Bank Bone

Archives of Surgery , Volume 109 (4) – Oct 1, 1974

Loading next page...
 
/lp/american-medical-association/excitation-transfer-in-bone-deleterious-effects-of-cobalt-60-radiation-iwbAk6Jgai
Publisher
American Medical Association
Copyright
Copyright © 1974 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1974.01360040012004
Publisher site
See Article on Publisher Site

Abstract

Abstract In a bone alloimplant in muscle, the structure of the organic matrix evocates or promotes a morphogenetic response from migratory mesenchymal cells. Radiation sterilization destroys the morphogenetic property at a much lower dose of cobalt 60 in undemineralized than in the demineralized bone. Damage to the organic matrix is heightened by excitation transfer from the radiated bone mineral to the matrix proteins. The mineral having an apatite structure also has a relatively high ionization potential and generates deleterious free radicals, which convert the matrix collagen to gelatin. Radiolysis prevents neither the undesirably delayed hypersensitivity response nor the degradation of the bone morphogenetic property by endogenous protease. These observations account for the failure of freeze-dried and radiation-sterilized bone to promote repair of large bone defects in adult patients. References 1. Buring K, Urist MR: Effects of ionizing radiation on the bone induction principle in matrix of bone implants . Clin Orthop 55:225-234, 1967. 2. Buring K: Ionizing radiation for sterilization of bone , in Sterilization and Preservation of Biological Tissues by Ionizing Radiation . Vienna, International Atomic Energy Agency, 1970, pp 71-78. 3. Santin LN: Sterilization of bone tissue with gamma rays of cobalt 60 . Radiobiologiia 3:621-625, 1963. 4. Volkov M, Bizer V: Homeotransplantation of bone tissue in children . Moscow, MIR Publishers, 1972, p 232. 5. Bailey AJ, Tromas WJ: Effects of ionizing radiation on the ultrastructure of collagen fibrils . Radiat Res 23:145-155, 1964.Crossref 6. Czerniewski M, et al: Bone tissue sterilization using reactor fuel gamma radiation . Nukleonika 10:19-28, 1973. 7. Wright KA, Trump JG: Cooperative studies in the use of ionizing radiation for sterilization and preservation of biological tissues , in Sterilization and Preservation of Biological Tissues by Ionizing Radiation . Vienna, International Atomic Energy Agency, 1970, pp 107-118. 8. Ostrowski K: Current problems of tissue banking . Transplant Proc 1:126-131, 1969. 9. Ostrowski K, Moskalewski S, Komender J: Teoretyczne aspekty sterylizacji promieniowaniem jonizujacym przeszczepow dla potrzeb . Klin Chir Narzadow Ruchia Orthop Pol 33:311-320, 1968. 10. Cohen J: Cathode ray sterilization of bone grafts . Arch Surg 71:784-789, 1955.Crossref 11. Bassett CAL, Packard AG Jr: A clinical assay of cathode ray sterilized bone grafts . Acta Orthop Scand 28:198-211, 1959.Crossref 12. Marmour L: Bone banks: A new concept in procurement and storage for homologous grafts . Calif Med 92:407-408, 1960. 13. Turner TC, Bassett CB, Sawyer PN: Sterilization of preserved bone grafts by high voltage cathode-irradiation . J Bone Joint Surg 38-A:862-884, 1956. 14. Urist MR: Bone: Formation by autoinduction . Science 150:893-899, 1965.Crossref 15. Urist MR: The substratum for bone morphogenesis . Dev Biol , (suppl 4) , pp 125-163, 1970. 16. Urist MR, et al: Quantitation of new bone formation in intramuscular implants of bone matrix in rabbits . Clin Orthop 68:279-293, 1970.Crossref 17. Urist MR, et al: The bone induction principle . Clin Orthop 53:243-283, 1967.Crossref 18. Urist MR, Strates BS: Bone morphogenetic protein . J Dent Res 50:1392-1406, 1971.Crossref 19. Van de Putte KA, Urist MR: Osteogenesis in the interior of intramuscular implants of decalcified bone matrix . Clin Orthop 43:257-270, 1966.Crossref 20. Urist MR, et al: Bone morphogenesis in implants of insoluble bone gelatin . Proc Natl Acad Sci USA 70:3511-3515, 1973.Crossref 21. Urist MR, et al: Bone morphogenesis in implants of residues of radioisotope labelled bone matrix. Calcif Tissue Res, to be published. 22. Urist MR, Iwata H: Preservation and biodegradation of the morphogenetic property of bone matrix . J Theor Biol 38:155-167, 1973.Crossref 23. Urist MR: Enzymes in bone morphogenesis: Endogenous enzymic degradation of the morphogenetic property in bone in solutions buffered by ethylenediaminetetraacetic acid (EDTA) , in Hard Tissue Growth, Repair and Remineralization, Ciba Symposium 11 . Amsterdam, Associated Scientific Publication, 1973, pp 143-160. 24. Urist MR, et al: Observations implicating an extracellular enzymic mechanism of control of bone morphogenesis . J Histochem Cytochem 22:88-103, 1974.Crossref 25. Mikulski AJ, Urist MR: Control of bone morphogenesis by a matrix glycopeptide and protease , abstracted. J Dent Res 53 (special issue):138, 1974.Crossref 26. Iwata H, Urist MR: Protein polysaccharide of bone morphogenetic matrix . Clin Orthop 87:257-274, 1972.Crossref 27. Christensen EA: Bacterial evaluation of sterilization procedures . Ann Pharm Fr 5:3-12, 1966. 28. Ostrowski K: Current problems of tissue banking . Transplant Proc 1:126-131, 1969. 29. Houben JL: Free radicals produced by ionizing radiation in bone and its constituents . Int J Radiat Biol 20:373-389, 1971.Crossref 30. Termine JD, Pullman I, Posner AS: Electron spin resonance study of irradiated bone and its constituents . Arch Biochem Biophys 122:318-330, 1967.Crossref 31. Ostrowski K, et al: Application of electron spin resonance in research on mineralized tissues . Clin Orthop 97:213-224, 1973.Crossref 32. Becker RO: Electron paramagnetic resonance in non-irradiated bone . Nature 199:1304-1305, 1963.Crossref 33. Gordy W, Ard WB, Shields H: Microwave spectroscopy of biological substances: I. Paramagnetic resonance of X-irradiated amino acids and proteins . Proc Natl Acad Sci USA 41:983-1004, 1955.Crossref 34. Fischer BV, et al: Radiation damage in calcium phosphates and collagen: An interpretation of ESR spectra . Radiat Res 46:229-235, 1971.Crossref 35. Stachowicz W, et al: On free radicals evoked by radiosterilization in preserved bone grafts , in Sterilization and Preservation of Biological Tissues by Ionizing Radiation . Vienna, International Atomic Energy Agency, 1970, pp 15-27. 36. Slager UT, Zucker MJ, Reilly EB: The persistance of electron spin resonance in bone grafts sterilized by ionizing radiation . Radiat Res 22:556-563, 1964.Crossref 37. Stachowicz W, et al: ESR study of bone tissue sterilized by gamma irradiation . Nukleonika 15:131-142, 1970. 38. Stachowicz W, et al: Deproteinized bone powder as a dosimeter for radiosterilization of biostatic grafts . Nukleonika 17:425-430, 1972. 39. Swartz HM: Long-lived electron spin resonance in rats irradiated at room temperature . Radiat Res 24:579-586, 1965.Crossref 40. Bonar LC, Glimcher MJ: Thermal denaturation of mineralized and demineralized bone collagens . J Ultrastruct Res 32:545-551, 1970.Crossref 41. Slager UT, Zucker MJ: The occurrence of electron spin resonance signals in bone grafts sterilized with high voltage electron beams . Transplant Bull 30:536-563, 1962.Crossref 42. Kausch W: Ueber knochenimplantation . Verh Dtsch Ges Chir 35:179-188, 1906. 43. Kausch W: Zur frage der freien transplantation toten knochens . Zentralbl Chir 36:1379-1381, 1909. 44. Kausch W: Ueber knochenersatz: Beitrage zur transplantation toten knochens . Beitr Klin Chir 68:670-716, 1910. 45. Gallie WE, Robertson DE: The transplantation of bone . JAMA 70:1134-1140, 1919.Crossref 46. Leriche R: Sur les greffes d'os mort et sur les greffes onoplastiques et heteroplastiques . Mem Acad Chir 76:389-410, 1950. 47. Rocher HL: Dead bone grafts in orthopaedic surgery . J Bone Joint Surg 35-B:328-329, 1953. 48. Burwell RG: The fate of bone grafts , in Apley AG (ed): Recent Advances in Orthopaedics . Baltimore, Williams & Wilkins Co, 1969, pp 115-207. 49. Lance EM: Bone and cartilage , in Najarian JS, Simmons RL (eds): Transplantation . Philadelphia, Lea & Febiger, 1972, pp 655-697. 50. Chalmers J: Transplantation immunity in bone homografting . J Bone Joint Surg 41-B:160-179, 1959. 51. Heiple KG, Chase SN, Herndon CH: A comparative study of the healing process following different types of bone transplantation . J Bone Joint Surg 45-A:1593-1616, 1963. 52. Bonfiglio M, Jeter WS: Immunological responses to bone . Clin Orthop 87:19-27, 1972. 53. Urist MR: Osteoinduction in undemineralized bone implants modified by chemical inhibitors of endogenous matrix enzymes . Clin Orthop 87:132-137, 1972.Crossref 54. Immaliev AS: The preparation preservation and transplantation of articular bone ends , in Apley AG (ed): Recent Advances in Orthopaedics . Baltimore, Williams & Wilkins Co, 1969, pp 209-263. 55. Okada S: Radiation Biochemistry . New York, Academic Press Inc, 1970. 56. Shore JS, Tobert BM, Sauerlich HE: The effects of ionizing radiation on trypsin: I. Losses of esterase and protease activities of trypsin and trypsin inhibitor compound . Arch Biochem Biophys 102:125-131, 1963.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Oct 1, 1974

References