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Excimer Laser Effects on Human Corneal Endothelium: Modulation by Serum Factor(s)

Excimer Laser Effects on Human Corneal Endothelium: Modulation by Serum Factor(s) Abstract Objective: To determine the possibility of endothelial cell damage after excimer laser ablation. Methods: Endothelial cell densities and morphology of human corneas after photoablations or mechanical keratectomy were compared with those of the untreated mates after 1 week of culture with or without serum. Results: Corneas cultured in serum-free medium after ablation to a depth of 150 μm showed endothelial cell densities reduced to 60% of untreated, mate corneas; ultrastructural analysis showed endothelial cell damage not seen in untreated mates. Corneas ablated to the same depth and cultured in serum-enriched medium showed no endothelial cell density loss, nor did corneas cultured in serum-free medium after an ablation to a depth of 50 μm or mechanical keratectomies averaging 95 μm. Conclusions: Endothelial cell loss in deep laser resections may be prevented by factor(s) in fetal bovine serum. The apparent lack of cell loss in clinical studies may be related to the protective action of similar factors in aqueous humor. References 1. Binder P. Excimer laser photoablation and radial keratotomy for the correction of myopia . Refract Corneal Surg . 1994;10:443-464. 2. Binder P, Anderson J, Rock M, Vrabec M. Human excimer laser keratectomy: clinical and histopathologic correlations . Ophthalmology . 1994;101:979-989.Crossref 3. Hersh P, Spinak A, Garrana R, Mayers M. Phototherapeutic keratectomy: strategies and results in 12 eyes . Refract Corneal Surg . 1993;9:S90-S95. 4. Krueger R, Binder P, McDonnell P. The effects of excimer laser photoablation on the cornea . In: Salz J, ed. Corneal Laser Surgery . St Louis, Mo: MosbyYear Book Inc; 1994:45-66. 5. Anderson JA, Rock M, Vrabec M, Binder PS. Human escimer laser keractectomy: immunohistochemical analysis . Arch Ophthalmol . 1996;114:54-60.Crossref 6. Seiler T, Derse M, Pham T. Repeated excimer laser treatment after photorefractive keratectomy . Arch Ophthalmol . 1992;110:1230-1233.Crossref 7. Tuft SJ, Gartry DS, Rawe IM, Meek KM. Photorefractive keratectomy: implications of corneal wound healing . Br J Ophthalmol . 1993;77:243-247.Crossref 8. Gaster R, Binder P, Coalwell K, Berns M, McCord R, Burstein N. Corneal surface ablation by 193 nm excimer laser and wound healing in rabbits . Invest Ophthalmol Vis Sci . 1989;30:90-98. 9. Dehm EJ, Puliafito CA, Adler CM, Steinert RF. Corneal endothelial injury in rabbits following excimer laser ablation at 193 and 248 nm . Arch Ophthalmol . 1986;104:1364-1368.Crossref 10. Marshall J, Trokel S, Rothery S. An ultrastructural study of corneal incisions induced by an excimer laser at 193 nm . Ophthalmology . 1985;92:749-758.Crossref 11. Koch JW, Lang GK, Naumann GO. Endothelial reaction to perforating and nonperforating excimer laser incisions in rabbits . Refract Corneal Surg . 1991;7:214-222. 12. Krueger R, Krasinski J, Radzewicz C, Stonecipher K, Rowsey JJ. Photography of shock waves during excimer laser ablation of the cornea: effect of helium gas on propagation velocity . Cornea . 1993;12:330-334.Crossref 13. Bor Z, Hopp B, Racz B, et al. Plume emission, shock wave and surface wave formation during excimer laser ablation of the cornea . Refract Corneal Surg . 1993;9( (suppl) ):S111-S115. 14. Yashima Y, McAuliffe DJ, Jacques SL, Flotte TJ. Laser induced photoacoustic injury of the skin: effect of inertial confinement . Lasers Surg Med . 1991;11:62-68.Crossref 15. Yashima Y, McAuliffe DJ, Flotte TJ. Cell selectivity to laser-induced photo-acoustic injury of skin . Lasers Surg Med . 1990;10:280-283.Crossref 16. Ediger MN. Excimer-laser-induced fluorescence of rabbit cornea: radiometric measurement through the cornea . Lasers Surg Med . 1991;22:93-98.Crossref 17. Costagliola C, Balestrieri P, Fioretti F, et al. ArF 193 nm excimer laser corneal surgery as a possible risk factor in cataractogenesis . Exp Eye Res . 1994.58:453-457.Crossref 18. Carones F, Brancato R, Venturi E, Morico A. The corneal endothelium after myopic excimer laser photorefractive keratectomy . Arch Ophthalmol . 1994;112:920-924.Crossref 19. Mardelli PG, Piebenga LW, Matta CS, Hyde LL, Gira J. Corneal endothelial status 12 to 55 months after excimer laser photorefractive keratectomy . Ophthalmology . 1995;102:544-549.Crossref 20. Stulting RD, Thompson KP, Reddick DE, Lynn MJ, Artunduaga G, Dillon W. The effect of the 193 nm excimer laser photorefractive keratectomy (PRK) on the human corneal endothelium . Invest Ophthalmol Vis Sci . 1994;35:1650. 21. Pallikaris IG, Siganos DS. Excimer laser in situ keratomileusis and photorefractive keratectomy for correction of high myopia . J Refract Corneal Surg . 1994;10:498-510. 22. Richard NR, Anderson JA, Weiss J, Binder PS. Air/liquid corneal organ culture: a light microscopic study . Curr Eye Res . 1991;10:739-749.Crossref 23. Anderson JA, Richard NR, Rock ME, Binder PS. Requirement for vitamin A in long-term culture of human cornea . Invest Ophthalmol Vis Sci . 1993;34:3442-3449. 24. Anderson JA, Sipes NJ, Binder PS. Healing rates of corneal wounds in the presence of an RGD-containing peptide conjugate . J Cell Biochem . 1993;131 ( (suppl) ):17E. 25. Weiss J, Richard NR, Anderson JA, Binder PS. Endothelial wound healing in biphasic organ culture and the effects of epidermal growth factor . Invest Ophthalmol Vis Sci . 1990;31:53. 26. Fugina Y, Tanishima T. Actin in wound healing of rabbit corneal endothelium, II: study by nitrobenzoxadiazole-phalacidin method . Jpn J Ophthalmol . 1987;31:393-404. 27. Smolin G. A technique for staining and separating corneal endothelium . Am J Ophthalmol . 1975;65:232-236. 28. Rock ME, Moore M, Anderson JA, Binder PS. 3-D computer models of human keratocytes from keratoconus corneas . CLAO J . 1995;21:57-60. 29. Kang S, Kim E, Kim H, Yoon Y, Hong Y. Corneal endothelium F-actin changes after deep photorefractive keratectomy (PRK) in the rabbit . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S297. Abstract. 30. Kim J, Kim G, Oh J. Corneal endothelial damage after deep excimer laser ablation . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S298. Abstract. 31. Navea A, Maldondo M, Capdevila C, Cisneros A, Menazo J. Delayed endothelial response to extreme myopic excimer laser corneal photoablation . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S298. Abstract. 32. Capdevila C, Maldondo M, Navea A, Cisneros A, Menazo J. Corneal endothelial silver staining immediately after deep excimer laser photorefractive keratectomy . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S298. Abstract. 33. Sher N, Hardten D, Fundingsland B, et al. 193 nm excimer photorefractive keratectomy in high myopia . Ophthalmology . 1994;101:1575-1582.Crossref 34. Heitzmann J, Binder P, Kassar B, Nordan L. The correction of high myopia with the excimer laser . Arch Ophthalmol . 1993;111:1627-1634.Crossref 35. Pallikaris I, Papatzanaki M, Stathi E, Frenshock O, Georgiadis A. Laser in situ keratomileusis . Lasers Surg Med . 1990;10:463-468.Crossref 36. Chiba K, Oak SS, Tsubota K, Laing RA, Goldstein J, Hecht S. Morphometric analysis of corneal endothelium following radial keratotomy . J Cataract Refract Surg . 1987;13:263-267.Crossref 37. Salz J. Progressive endothelial cell loss following repeat radial keratotomy: a case report . Ophthalmic Surg . 1982;13:12. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Excimer Laser Effects on Human Corneal Endothelium: Modulation by Serum Factor(s)

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Publisher
American Medical Association
Copyright
Copyright © 1996 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1996.01100140697010
Publisher site
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Abstract

Abstract Objective: To determine the possibility of endothelial cell damage after excimer laser ablation. Methods: Endothelial cell densities and morphology of human corneas after photoablations or mechanical keratectomy were compared with those of the untreated mates after 1 week of culture with or without serum. Results: Corneas cultured in serum-free medium after ablation to a depth of 150 μm showed endothelial cell densities reduced to 60% of untreated, mate corneas; ultrastructural analysis showed endothelial cell damage not seen in untreated mates. Corneas ablated to the same depth and cultured in serum-enriched medium showed no endothelial cell density loss, nor did corneas cultured in serum-free medium after an ablation to a depth of 50 μm or mechanical keratectomies averaging 95 μm. Conclusions: Endothelial cell loss in deep laser resections may be prevented by factor(s) in fetal bovine serum. The apparent lack of cell loss in clinical studies may be related to the protective action of similar factors in aqueous humor. References 1. Binder P. Excimer laser photoablation and radial keratotomy for the correction of myopia . Refract Corneal Surg . 1994;10:443-464. 2. Binder P, Anderson J, Rock M, Vrabec M. Human excimer laser keratectomy: clinical and histopathologic correlations . Ophthalmology . 1994;101:979-989.Crossref 3. Hersh P, Spinak A, Garrana R, Mayers M. Phototherapeutic keratectomy: strategies and results in 12 eyes . Refract Corneal Surg . 1993;9:S90-S95. 4. Krueger R, Binder P, McDonnell P. The effects of excimer laser photoablation on the cornea . In: Salz J, ed. Corneal Laser Surgery . St Louis, Mo: MosbyYear Book Inc; 1994:45-66. 5. Anderson JA, Rock M, Vrabec M, Binder PS. Human escimer laser keractectomy: immunohistochemical analysis . Arch Ophthalmol . 1996;114:54-60.Crossref 6. Seiler T, Derse M, Pham T. Repeated excimer laser treatment after photorefractive keratectomy . Arch Ophthalmol . 1992;110:1230-1233.Crossref 7. Tuft SJ, Gartry DS, Rawe IM, Meek KM. Photorefractive keratectomy: implications of corneal wound healing . Br J Ophthalmol . 1993;77:243-247.Crossref 8. Gaster R, Binder P, Coalwell K, Berns M, McCord R, Burstein N. Corneal surface ablation by 193 nm excimer laser and wound healing in rabbits . Invest Ophthalmol Vis Sci . 1989;30:90-98. 9. Dehm EJ, Puliafito CA, Adler CM, Steinert RF. Corneal endothelial injury in rabbits following excimer laser ablation at 193 and 248 nm . Arch Ophthalmol . 1986;104:1364-1368.Crossref 10. Marshall J, Trokel S, Rothery S. An ultrastructural study of corneal incisions induced by an excimer laser at 193 nm . Ophthalmology . 1985;92:749-758.Crossref 11. Koch JW, Lang GK, Naumann GO. Endothelial reaction to perforating and nonperforating excimer laser incisions in rabbits . Refract Corneal Surg . 1991;7:214-222. 12. Krueger R, Krasinski J, Radzewicz C, Stonecipher K, Rowsey JJ. Photography of shock waves during excimer laser ablation of the cornea: effect of helium gas on propagation velocity . Cornea . 1993;12:330-334.Crossref 13. Bor Z, Hopp B, Racz B, et al. Plume emission, shock wave and surface wave formation during excimer laser ablation of the cornea . Refract Corneal Surg . 1993;9( (suppl) ):S111-S115. 14. Yashima Y, McAuliffe DJ, Jacques SL, Flotte TJ. Laser induced photoacoustic injury of the skin: effect of inertial confinement . Lasers Surg Med . 1991;11:62-68.Crossref 15. Yashima Y, McAuliffe DJ, Flotte TJ. Cell selectivity to laser-induced photo-acoustic injury of skin . Lasers Surg Med . 1990;10:280-283.Crossref 16. Ediger MN. Excimer-laser-induced fluorescence of rabbit cornea: radiometric measurement through the cornea . Lasers Surg Med . 1991;22:93-98.Crossref 17. Costagliola C, Balestrieri P, Fioretti F, et al. ArF 193 nm excimer laser corneal surgery as a possible risk factor in cataractogenesis . Exp Eye Res . 1994.58:453-457.Crossref 18. Carones F, Brancato R, Venturi E, Morico A. The corneal endothelium after myopic excimer laser photorefractive keratectomy . Arch Ophthalmol . 1994;112:920-924.Crossref 19. Mardelli PG, Piebenga LW, Matta CS, Hyde LL, Gira J. Corneal endothelial status 12 to 55 months after excimer laser photorefractive keratectomy . Ophthalmology . 1995;102:544-549.Crossref 20. Stulting RD, Thompson KP, Reddick DE, Lynn MJ, Artunduaga G, Dillon W. The effect of the 193 nm excimer laser photorefractive keratectomy (PRK) on the human corneal endothelium . Invest Ophthalmol Vis Sci . 1994;35:1650. 21. Pallikaris IG, Siganos DS. Excimer laser in situ keratomileusis and photorefractive keratectomy for correction of high myopia . J Refract Corneal Surg . 1994;10:498-510. 22. Richard NR, Anderson JA, Weiss J, Binder PS. Air/liquid corneal organ culture: a light microscopic study . Curr Eye Res . 1991;10:739-749.Crossref 23. Anderson JA, Richard NR, Rock ME, Binder PS. Requirement for vitamin A in long-term culture of human cornea . Invest Ophthalmol Vis Sci . 1993;34:3442-3449. 24. Anderson JA, Sipes NJ, Binder PS. Healing rates of corneal wounds in the presence of an RGD-containing peptide conjugate . J Cell Biochem . 1993;131 ( (suppl) ):17E. 25. Weiss J, Richard NR, Anderson JA, Binder PS. Endothelial wound healing in biphasic organ culture and the effects of epidermal growth factor . Invest Ophthalmol Vis Sci . 1990;31:53. 26. Fugina Y, Tanishima T. Actin in wound healing of rabbit corneal endothelium, II: study by nitrobenzoxadiazole-phalacidin method . Jpn J Ophthalmol . 1987;31:393-404. 27. Smolin G. A technique for staining and separating corneal endothelium . Am J Ophthalmol . 1975;65:232-236. 28. Rock ME, Moore M, Anderson JA, Binder PS. 3-D computer models of human keratocytes from keratoconus corneas . CLAO J . 1995;21:57-60. 29. Kang S, Kim E, Kim H, Yoon Y, Hong Y. Corneal endothelium F-actin changes after deep photorefractive keratectomy (PRK) in the rabbit . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S297. Abstract. 30. Kim J, Kim G, Oh J. Corneal endothelial damage after deep excimer laser ablation . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S298. Abstract. 31. Navea A, Maldondo M, Capdevila C, Cisneros A, Menazo J. Delayed endothelial response to extreme myopic excimer laser corneal photoablation . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S298. Abstract. 32. Capdevila C, Maldondo M, Navea A, Cisneros A, Menazo J. Corneal endothelial silver staining immediately after deep excimer laser photorefractive keratectomy . Invest Ophthalmol Vis Sci . 1995;36( (suppl 4) ):S298. Abstract. 33. Sher N, Hardten D, Fundingsland B, et al. 193 nm excimer photorefractive keratectomy in high myopia . Ophthalmology . 1994;101:1575-1582.Crossref 34. Heitzmann J, Binder P, Kassar B, Nordan L. The correction of high myopia with the excimer laser . Arch Ophthalmol . 1993;111:1627-1634.Crossref 35. Pallikaris I, Papatzanaki M, Stathi E, Frenshock O, Georgiadis A. Laser in situ keratomileusis . Lasers Surg Med . 1990;10:463-468.Crossref 36. Chiba K, Oak SS, Tsubota K, Laing RA, Goldstein J, Hecht S. Morphometric analysis of corneal endothelium following radial keratotomy . J Cataract Refract Surg . 1987;13:263-267.Crossref 37. Salz J. Progressive endothelial cell loss following repeat radial keratotomy: a case report . Ophthalmic Surg . 1982;13:12.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Dec 1, 1996

References