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Examination of Normal and Myxedematous Skin: Use of Mowry's Alcian Blue-Periodic Acid-Schiff Technique

Examination of Normal and Myxedematous Skin: Use of Mowry's Alcian Blue-Periodic Acid-Schiff... Abstract Recent investigations have demonstrated a notable increase of certain polysaccharides in the cutaneous lesions of myxedema as compared with normal skin.1-3 In the present study Alcian blue (AB), which stains substances believed to be acid mucopolysaccharides,4-6 was combined with the periodic acid-Schiff (PAS) stain, which colors 1,2glycols, for the microscopical examination of normal skin and skin from patients with circumscribed or pretibial myxedema, true myxedema, and lichen myxedematosus. Materials and Methods Formalin-fixed specimens6 of involved skin from four patients with circumscribed or pretibial myxedema, three with true myxedema, one with lichen myxedematosus, and several of normal skin (all specimens were from adults) were sectioned and treated with the Alcian blue-periodic acidSchiff combination (AB-PAS) according to the technique of Mowry.7 Observations Mowry's fundamental work with Alcian blue alone and in combination with other stains6-8 has demonstrated that the AB-PAS combination stains References 1. Asboe-Hansen, G.: Some Systemic Connective Tissue Disorders Pertaining to Dermatology , in Connective Tissue in Health and Disease , Copenhagen, Ejnar Munksgaards Forlag, 1954. 2. Watson, E. M.: Localized (Pretibial) Myxœdema, Associated with Graves' Disease , Canad. M. A. J. 54:260 ( (March) ) 1946. 3. Watson, E. M., and Pearce, R. H.: The Mucopolysaccharide Content of the Skin in Localized (Pretibial) Myxedema , Am. J. Clin. Path. 19:442 ( (May) ) 1949. 4. Lison, L.: Alcian Blue 8G with Chlorantine Fast Red 5B: A Technic for Selective Staining of Mucopolysaccharides , Stain Technol. 29:131 ( (May) ) 1954. 5. McManus, J. F. A.: Histochemistry of Connective Tissue , in Asboe-Hansen, G.: Connective Tissue in Health and Disease , Copenhagen, Ejnar Munksgaards Forlag, 1954. 6. Mowry, R. W.: Alcian Blue Technics for the Histochemical Study of Acid Carbohydrates , J. Histochem. 4:407 ( (Sept.) ) 1956. 7. Mowry, R. W.: The Coloration of Acidic Carbohydrates of Bacteria and Fungi in Tissue Sections with Special Reference to Capsules of Cryptococcus Neoformans, Pneumococci, and Staphylococci , Am. J. Path. 32:628 ( (May) -June) 1956. 8. Mowry, R. W.: Personal communication to the authors. 9. Dalton, J. E., and Seidell, M. A.: Studies on Lichen Myxedematosus (Papular Mucinosis) , A.M. A. Arch. Dermat. & Syph. 67:194 ( (Feb.) ) 1953. 10. Montagna, W.: The Structure and Function of Skin , New York, Academic Press, Inc., 1956. 11. Sylven, B.: The Qualitative Distribution of Metachromatic Polysaccharide Material During Hair Growth , Exper. Cell Res. 1:582 ( (Nov.) ) 1950. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives of Dermatology American Medical Association

Examination of Normal and Myxedematous Skin: Use of Mowry's Alcian Blue-Periodic Acid-Schiff Technique

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Publisher
American Medical Association
Copyright
Copyright © 1957 American Medical Association. All Rights Reserved.
ISSN
0096-5359
DOI
10.1001/archderm.1957.01550230001001
Publisher site
See Article on Publisher Site

Abstract

Abstract Recent investigations have demonstrated a notable increase of certain polysaccharides in the cutaneous lesions of myxedema as compared with normal skin.1-3 In the present study Alcian blue (AB), which stains substances believed to be acid mucopolysaccharides,4-6 was combined with the periodic acid-Schiff (PAS) stain, which colors 1,2glycols, for the microscopical examination of normal skin and skin from patients with circumscribed or pretibial myxedema, true myxedema, and lichen myxedematosus. Materials and Methods Formalin-fixed specimens6 of involved skin from four patients with circumscribed or pretibial myxedema, three with true myxedema, one with lichen myxedematosus, and several of normal skin (all specimens were from adults) were sectioned and treated with the Alcian blue-periodic acidSchiff combination (AB-PAS) according to the technique of Mowry.7 Observations Mowry's fundamental work with Alcian blue alone and in combination with other stains6-8 has demonstrated that the AB-PAS combination stains References 1. Asboe-Hansen, G.: Some Systemic Connective Tissue Disorders Pertaining to Dermatology , in Connective Tissue in Health and Disease , Copenhagen, Ejnar Munksgaards Forlag, 1954. 2. Watson, E. M.: Localized (Pretibial) Myxœdema, Associated with Graves' Disease , Canad. M. A. J. 54:260 ( (March) ) 1946. 3. Watson, E. M., and Pearce, R. H.: The Mucopolysaccharide Content of the Skin in Localized (Pretibial) Myxedema , Am. J. Clin. Path. 19:442 ( (May) ) 1949. 4. Lison, L.: Alcian Blue 8G with Chlorantine Fast Red 5B: A Technic for Selective Staining of Mucopolysaccharides , Stain Technol. 29:131 ( (May) ) 1954. 5. McManus, J. F. A.: Histochemistry of Connective Tissue , in Asboe-Hansen, G.: Connective Tissue in Health and Disease , Copenhagen, Ejnar Munksgaards Forlag, 1954. 6. Mowry, R. W.: Alcian Blue Technics for the Histochemical Study of Acid Carbohydrates , J. Histochem. 4:407 ( (Sept.) ) 1956. 7. Mowry, R. W.: The Coloration of Acidic Carbohydrates of Bacteria and Fungi in Tissue Sections with Special Reference to Capsules of Cryptococcus Neoformans, Pneumococci, and Staphylococci , Am. J. Path. 32:628 ( (May) -June) 1956. 8. Mowry, R. W.: Personal communication to the authors. 9. Dalton, J. E., and Seidell, M. A.: Studies on Lichen Myxedematosus (Papular Mucinosis) , A.M. A. Arch. Dermat. & Syph. 67:194 ( (Feb.) ) 1953. 10. Montagna, W.: The Structure and Function of Skin , New York, Academic Press, Inc., 1956. 11. Sylven, B.: The Qualitative Distribution of Metachromatic Polysaccharide Material During Hair Growth , Exper. Cell Res. 1:582 ( (Nov.) ) 1950.

Journal

A.M.A. Archives of DermatologyAmerican Medical Association

Published: Nov 1, 1957

References