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Enhanced Interaction of Patients' Lymphocytes With Human Dermal Microvascular Endothelial Cell Cultures in Active Adamantiades-Behçet Disease

Enhanced Interaction of Patients' Lymphocytes With Human Dermal Microvascular Endothelial Cell... Abstract Background and Design: To elucidate the role of lymphocyte/endothelial cell interactions in patients with Adamantiades-Behçet disease (ABD), we studied 16 patients of German and Turkish nationality (aged 18-57 years), all with active ABD, and 12 healthy volunteers (controls) of similar age and nationality. Peripheral blood lymphocytes (PBL) of patients were coincubated with human dermal microvascular endothelial cells (HDMEC) and human keratinocytes (HK) in vitro; interactions of PBL with HDMEC and HK were investigated using an established fluorometric assay. Interactions of patients' PBL with HDMEC, HK, or both were the main outcome measures. Results: A significant increase of fluorescence with increasing PBL/HDMEC ratios was seen in patients and controls (P<.001); patients showed a significantly higher increase of fluorescence at higher PBL/HDMEC ratios (P<.05). The PBL/HK coincubation did not show significant alterations compared with the basal fluorescence signals of HK monolayers alone. Peripheral blood lymphocyte and HDMEC fluorescence values that were more than 2 SDs of controls (defined as positive result of assay) were found in a significantly higher number of patients with 2 or more active symptoms at the time of investigation (83%) compared with patients with only 1 active symptom (10%) (P=.008). Other clinical data did not correlate with the results of the PBL/HDMEC coincubation assay. Conclusions: Our results indicate enhanced in vitro interaction of PBL from patients with ABD with HDMEC, which was additionally shown to be a marker of the activity of the disease.Arch Dermatol. 1996;132:1323-1329 References 1. Adamantiades B. Sur un cas d'iritis à hypopyon récidivant . Ann Oculist (Paris) . 1931;168:271-278. 2. Behçet H. Über rezidivierende, aphthose, durch ein Virus verursachte Geschwüre am Mund, am Auge und an den Genitalien . Dermatol Wochenschr. 1937;105:1152-1157. 3. O'Duffy JD. Treatment and prognosis . In: Plotkin GR, Calabro JJ, O'Duffy JD, eds. Behçet's Disease: A Contemporary Synopsis . Armonk, NY: Futura Publishing Co Inc; 1988:295-303. 4. Zouboulis ChC, Kurz K, Bratzke B, Orfanos CE. Morbus Adamantiades-Behçet: nekrotisierende Systemvaskulitis mit letalem Ausgang . Hautarzt. 1991;42:451-454. 5. Wechsler B, Pietti JC. Behçet's disease: retains most of its mysteries . BMJ. 1992;304:1199-1200.Crossref 6. International Study Group for Behçet's Disease. Criteria for diagnosis of Behçet's disease . Lancet. 1990;335:1078-1080. 7. Jorizzo JL, Solomon AR, Zanolli MD, Leshin B. Neutrophilic vascular reactions . J Am Acad Dermatol. 1988;19:983-1005.Crossref 8. Kienbaum S, Zouboulis ChC, Waibel M, Orfanos CE. Chemotactic neutrophilic vasculitis: a new histopathological pattern of vasculitis found in mucocutaneous lesions of patients with Adamantiades-Behçet's disease . In: Wechsler B, Godeau P, eds. Behçet's Disease . Amsterdam, the Netherlands: Excerpta Medica; 1993:337-341. 9. Chun SI, Su WPD, Lee S. Histopathologic study of cutaneous lesions in Behçet's syndrome . J Dermatol. 1990;17:333-341. 10. O'Duffy JD. Vasculitis in Behçet's disease . Rheum Dis Clin North Am. 1990; 16:423-431. 11. Dolby AE. Recurrent aphthous ulceration: effect of sera and peripheral blood lymphocytes upon oral epithelial tissue culture cells . Immunology. 1969;17: 709-714. 12. Reimer G, Steinkohl S, Djawari D, Hornstein OP. Lytic effect of cytotoxic lymphocytes on oral epithelial cells in Behçet's disease . Br J Dermatol. 1982;107: 529-536.Crossref 13. Rogers RS, Sams WM, Shorter RG. Lymphocytotoxicity in recurrent aphthous stomatitis . Arch Dermatol. 1974;109:361-363.Crossref 14. Ayed K, Hamzaoui K, Hamza M, Ben Ayed H. Augmentation de l'activité 'natural killer' au cours de la maladie de Behçet . Presse Med. 1985;14: 2299-2300. 15. Lee BK, Choi IH, Shim WN, et al. Study on cell-mediated immunity in patients with Behçet's syndrome . Korean J Immunol. 1985;10:118-121. 16. Watanabe K, Ohashi Y. Natural killer cell activity in patients with Behçet's disease . Am J Ophthalmol. 1984;98:813-814. 17. Kaneko F, Takahashi Y, Muramatsu R, Miura Y. Natural killer cell numbers and function in peripheral lymphoid cells in Behçet's disease . Br J Dermatol. 1985; 113:313-318.Crossref 18. Hamzaoui K, Ayed KH, Hamza M, Touraine JL. Natural killer cells in Behçet's disease . Clin Exp Immunol. 1988;71:126-131. 19. Hamzaoui K, Ayed K, Slim A, Hamza M, Touraine JL. Natural killer cell activity, interferon-gamma and antibodies to herpes viruses in patients with Behçet's disease . Clin Exp Immunol. 1990;79:28-34.Crossref 20. Young C, Lehner T, Barnes G. CD4 and CD8 cell responses to herpes simplex virus in Behçet's disease . Clin Exp Immunol. 1988;73:6-10. 21. Hamzaoui K, Kahan A, Ayed K, Hamza M. Cytotoxic T cells against herpes simplex virus in Behçet's disease . Clin Exp Immunol. 1990;81:390-395.Crossref 22. Ruszczak Z, Detmar M, Imcke E, Orfanos CE. Effects of rIFN alpha, beta, and gamma on the morphology, proliferation, and cell surface antigen expression of human dermal microvascular endothelial cells in vitro . J Invest Dermatol. 1990;95:693-699.Crossref 23. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizating colonies from single cells . Cell. 1975; 6:331-334.Crossref 24. Stadler R, Detmar M, Stephanek K, Bangemann Ch, Orfanos CE. A rapid fluorometric assay for the determination of keratinocyte proliferation in vitro . J Invest Dermatol. 1989;93:532-534.Crossref 25. Zouboulis ChC, Garbe C, Krasagakis K, Krüger S, Orfanos CE. A fluorometric rapid microassay to identify antiproliferative compounds for human melanoma cells in vitro . Melanoma Res. 1991;1:91-95.Crossref 26. Krüger-Krasagakes S, Garbe C, Kossman P, Orfanos CE. A rapid and sensitive fluorometric microassay for determining cell mediated cytotoxicity to adherent growing cell lines . J Immunol Methods. 1993;156:1-8.Crossref 27. Kahan A, Hamzaoui K, Khaled A. Abnormalities of T-lymphocyte subsets in Behçet's disease demonstrated with anti-CD45RA and anti-CD29 monoclonal antibodies . J Rheumatol. 1992;19:742-746. 28. Hayrinen-Immonen R, Malmstrom M, Nordstrom D, Sorsa T, Konttinen T. Distribution of adhesion receptors in recurrent oral ulcers . J Oral Pathol Med. 1992;21:199-202.Crossref 29. Kienbaum S, Zouboulis ChC, Waibel M, Orfanos CE. Papulopustular skin lesions in Adamantiades-Behçet's disease show a similar histopathological pattern as the classical mucocutaneous manifestations . In: Wechsler B, Godeau P, eds. Behçet's Disease . Amsterdam, the Netherlands: Excerpta Medica; 1993: 331-336. 30. Özgün S, Akoglu T, Direskeneli H, Yurkadul S. Neutrophil adhesion to endothelial cells in patients with Behçet's disease . In: Wechsler B, Godeau P, eds. Behçet's Disease . Amsterdam, the Netherlands: Excerpta Medica; 1993:135-138. 31. Cavender DE. Lymphocyte adhesion to endothelial cells in vitro: models for the study of normal lymphocyte recirculation and lymphocyte emigration into chronic inflammatory lesions . J Invest Dermatol. 1989;93( (suppl) ):88S-95S.Crossref 32. Hunyadi J, Simon M, Kenderessy AS, Olah J, Dobozy A. Expression of adhesion molecules and monocyte markers on cultured human keratinocytes . Eur J Dermatol. 1992;2:50-55. 33. Nickoloff BJ, Griffiths EM, Barker JN. The role of adhesion molecules, chemotactic factors, and cytokines in inflammatory and neoplastic skin disease: 1990 update . J Invest Dermatol. 1990;94( (suppl) ):151S-157S.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Enhanced Interaction of Patients' Lymphocytes With Human Dermal Microvascular Endothelial Cell Cultures in Active Adamantiades-Behçet Disease

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Publisher
American Medical Association
Copyright
Copyright © 1996 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1996.03890350065011
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Abstract

Abstract Background and Design: To elucidate the role of lymphocyte/endothelial cell interactions in patients with Adamantiades-Behçet disease (ABD), we studied 16 patients of German and Turkish nationality (aged 18-57 years), all with active ABD, and 12 healthy volunteers (controls) of similar age and nationality. Peripheral blood lymphocytes (PBL) of patients were coincubated with human dermal microvascular endothelial cells (HDMEC) and human keratinocytes (HK) in vitro; interactions of PBL with HDMEC and HK were investigated using an established fluorometric assay. Interactions of patients' PBL with HDMEC, HK, or both were the main outcome measures. Results: A significant increase of fluorescence with increasing PBL/HDMEC ratios was seen in patients and controls (P<.001); patients showed a significantly higher increase of fluorescence at higher PBL/HDMEC ratios (P<.05). The PBL/HK coincubation did not show significant alterations compared with the basal fluorescence signals of HK monolayers alone. Peripheral blood lymphocyte and HDMEC fluorescence values that were more than 2 SDs of controls (defined as positive result of assay) were found in a significantly higher number of patients with 2 or more active symptoms at the time of investigation (83%) compared with patients with only 1 active symptom (10%) (P=.008). Other clinical data did not correlate with the results of the PBL/HDMEC coincubation assay. Conclusions: Our results indicate enhanced in vitro interaction of PBL from patients with ABD with HDMEC, which was additionally shown to be a marker of the activity of the disease.Arch Dermatol. 1996;132:1323-1329 References 1. Adamantiades B. Sur un cas d'iritis à hypopyon récidivant . Ann Oculist (Paris) . 1931;168:271-278. 2. Behçet H. Über rezidivierende, aphthose, durch ein Virus verursachte Geschwüre am Mund, am Auge und an den Genitalien . Dermatol Wochenschr. 1937;105:1152-1157. 3. O'Duffy JD. Treatment and prognosis . In: Plotkin GR, Calabro JJ, O'Duffy JD, eds. Behçet's Disease: A Contemporary Synopsis . Armonk, NY: Futura Publishing Co Inc; 1988:295-303. 4. Zouboulis ChC, Kurz K, Bratzke B, Orfanos CE. Morbus Adamantiades-Behçet: nekrotisierende Systemvaskulitis mit letalem Ausgang . Hautarzt. 1991;42:451-454. 5. Wechsler B, Pietti JC. Behçet's disease: retains most of its mysteries . BMJ. 1992;304:1199-1200.Crossref 6. International Study Group for Behçet's Disease. Criteria for diagnosis of Behçet's disease . Lancet. 1990;335:1078-1080. 7. Jorizzo JL, Solomon AR, Zanolli MD, Leshin B. Neutrophilic vascular reactions . J Am Acad Dermatol. 1988;19:983-1005.Crossref 8. Kienbaum S, Zouboulis ChC, Waibel M, Orfanos CE. Chemotactic neutrophilic vasculitis: a new histopathological pattern of vasculitis found in mucocutaneous lesions of patients with Adamantiades-Behçet's disease . In: Wechsler B, Godeau P, eds. Behçet's Disease . Amsterdam, the Netherlands: Excerpta Medica; 1993:337-341. 9. Chun SI, Su WPD, Lee S. Histopathologic study of cutaneous lesions in Behçet's syndrome . J Dermatol. 1990;17:333-341. 10. O'Duffy JD. Vasculitis in Behçet's disease . Rheum Dis Clin North Am. 1990; 16:423-431. 11. Dolby AE. Recurrent aphthous ulceration: effect of sera and peripheral blood lymphocytes upon oral epithelial tissue culture cells . Immunology. 1969;17: 709-714. 12. Reimer G, Steinkohl S, Djawari D, Hornstein OP. Lytic effect of cytotoxic lymphocytes on oral epithelial cells in Behçet's disease . Br J Dermatol. 1982;107: 529-536.Crossref 13. Rogers RS, Sams WM, Shorter RG. Lymphocytotoxicity in recurrent aphthous stomatitis . Arch Dermatol. 1974;109:361-363.Crossref 14. Ayed K, Hamzaoui K, Hamza M, Ben Ayed H. Augmentation de l'activité 'natural killer' au cours de la maladie de Behçet . Presse Med. 1985;14: 2299-2300. 15. Lee BK, Choi IH, Shim WN, et al. Study on cell-mediated immunity in patients with Behçet's syndrome . Korean J Immunol. 1985;10:118-121. 16. Watanabe K, Ohashi Y. Natural killer cell activity in patients with Behçet's disease . Am J Ophthalmol. 1984;98:813-814. 17. Kaneko F, Takahashi Y, Muramatsu R, Miura Y. Natural killer cell numbers and function in peripheral lymphoid cells in Behçet's disease . Br J Dermatol. 1985; 113:313-318.Crossref 18. Hamzaoui K, Ayed KH, Hamza M, Touraine JL. Natural killer cells in Behçet's disease . Clin Exp Immunol. 1988;71:126-131. 19. Hamzaoui K, Ayed K, Slim A, Hamza M, Touraine JL. Natural killer cell activity, interferon-gamma and antibodies to herpes viruses in patients with Behçet's disease . Clin Exp Immunol. 1990;79:28-34.Crossref 20. Young C, Lehner T, Barnes G. CD4 and CD8 cell responses to herpes simplex virus in Behçet's disease . Clin Exp Immunol. 1988;73:6-10. 21. Hamzaoui K, Kahan A, Ayed K, Hamza M. Cytotoxic T cells against herpes simplex virus in Behçet's disease . Clin Exp Immunol. 1990;81:390-395.Crossref 22. Ruszczak Z, Detmar M, Imcke E, Orfanos CE. Effects of rIFN alpha, beta, and gamma on the morphology, proliferation, and cell surface antigen expression of human dermal microvascular endothelial cells in vitro . J Invest Dermatol. 1990;95:693-699.Crossref 23. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizating colonies from single cells . Cell. 1975; 6:331-334.Crossref 24. Stadler R, Detmar M, Stephanek K, Bangemann Ch, Orfanos CE. A rapid fluorometric assay for the determination of keratinocyte proliferation in vitro . J Invest Dermatol. 1989;93:532-534.Crossref 25. Zouboulis ChC, Garbe C, Krasagakis K, Krüger S, Orfanos CE. A fluorometric rapid microassay to identify antiproliferative compounds for human melanoma cells in vitro . Melanoma Res. 1991;1:91-95.Crossref 26. Krüger-Krasagakes S, Garbe C, Kossman P, Orfanos CE. A rapid and sensitive fluorometric microassay for determining cell mediated cytotoxicity to adherent growing cell lines . J Immunol Methods. 1993;156:1-8.Crossref 27. Kahan A, Hamzaoui K, Khaled A. Abnormalities of T-lymphocyte subsets in Behçet's disease demonstrated with anti-CD45RA and anti-CD29 monoclonal antibodies . J Rheumatol. 1992;19:742-746. 28. Hayrinen-Immonen R, Malmstrom M, Nordstrom D, Sorsa T, Konttinen T. Distribution of adhesion receptors in recurrent oral ulcers . J Oral Pathol Med. 1992;21:199-202.Crossref 29. Kienbaum S, Zouboulis ChC, Waibel M, Orfanos CE. Papulopustular skin lesions in Adamantiades-Behçet's disease show a similar histopathological pattern as the classical mucocutaneous manifestations . In: Wechsler B, Godeau P, eds. Behçet's Disease . Amsterdam, the Netherlands: Excerpta Medica; 1993: 331-336. 30. Özgün S, Akoglu T, Direskeneli H, Yurkadul S. Neutrophil adhesion to endothelial cells in patients with Behçet's disease . In: Wechsler B, Godeau P, eds. Behçet's Disease . Amsterdam, the Netherlands: Excerpta Medica; 1993:135-138. 31. Cavender DE. Lymphocyte adhesion to endothelial cells in vitro: models for the study of normal lymphocyte recirculation and lymphocyte emigration into chronic inflammatory lesions . J Invest Dermatol. 1989;93( (suppl) ):88S-95S.Crossref 32. Hunyadi J, Simon M, Kenderessy AS, Olah J, Dobozy A. Expression of adhesion molecules and monocyte markers on cultured human keratinocytes . Eur J Dermatol. 1992;2:50-55. 33. Nickoloff BJ, Griffiths EM, Barker JN. The role of adhesion molecules, chemotactic factors, and cytokines in inflammatory and neoplastic skin disease: 1990 update . J Invest Dermatol. 1990;94( (suppl) ):151S-157S.Crossref

Journal

Archives of DermatologyAmerican Medical Association

Published: Nov 1, 1996

References