Abstract To the Editor.— We have been able to detect circulating endotoxin in six of 11 patients with psoriasis.Our interest in the possibility of endotoxemia in psoriasis followed recent demonstrations of alterations in levels of alternative complement pathway components1 and of circulating IgA immune complexes in psoriasis.2 Endotoxin and immune IgA complexes each activate the alternative complement pathway.3,4Informed consent was obtained from 11 patients with psoriasis. Blood was drawn from each patient with a pyrogen-free syringe. The skin site for venipuncture was a sterilized antecubital fossa free of active psoriatic plaques.Endotoxin was measured by the Limulus lysate test on heparinized plasma following extraction by the chloroform method.5 A positive test result was indicated by firm clot formation after incubation at 37 °C for four hours. A weakly positive test result was indicated by a weak soft gel after four hours. Each test was matched References 1. Marley WM, Belew PW, Rosenberg EW, et al: Abnormalities in the alternative pathway of complement in psoriasis, abstracted . Clin Res 1981;29:605A. 2. Hall RP, Peck G, Lawley TJ: Detection of IgA immune complexes in patients with psoriasis, abstracted . Clin Res 1981;29:597A. 3. Pillemer L, Blum L, Lepow IH, et al: The properdin system and immunity: I. Demonstration and isolation of a new serum protein, properdin, and its role in immune phenomena . Science 1954;120:279-285.Crossref 4. Lachmann PJ, Nicol P: Reaction mechanism of the alternative pathway of complement fixation . Lancet 1973;1:465-467.Crossref 5. Levin J, Tomasulo PA, Oser RS: Detection of endotoxin in human blood and demonstration of an inhibitor . J Lab Clin Med 1970;75:903-911.
Archives of Dermatology – American Medical Association
Published: Mar 1, 1982