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Endothelial Damage in Monkeys After Radial Keratotomy Performed With a Diamond Blade

Endothelial Damage in Monkeys After Radial Keratotomy Performed With a Diamond Blade Abstract • Anterior radial keratotomy was performed with a diamond blade in ten rhesus monkey eyes. Results were compared with those of a previous study in which a metal blade was used. Histologic assessment showed endothelial degeneration, but fewer edematous endothelial cells, than in the previous study. Specular microscopy demonstrated statistically significant endothelial cell losses (7.99%), when preoperative and threemonth postoperative values were compared. Autoradiography showed little cell division in the endothelial cell layer. Cell loss seemed to be repaired mainly by the spreading of neighboring cells. Endothelial cell division is also limited in humans, and the cumulative loss of cells due to surgical trauma combined with continuous damage-related losses and later agerelated reductions in cell numbers could produce corneal decompensation in some patients years after radial keratotomy. References 1. Fyodorov SN, Durnev VV: Operation of dosaged dissection of corneal circular ligament in cases of myopia of mild degree . Ann Ophthalmol 1979;11:1885-1890. 2. Reddy PS, Reddy PR: Anterior keratotomy . Ophthalmic Surg 1980;11:765-767. 3. Hoffer KJ, Darian JJ, Pettit TH, et al: UCLA clinical trial of radial keratotomy . Ophthalmology 1981;88:729-736.Crossref 4. Bores LD, Myer W, Cowden J: Radial keratotomy; An analysis of the American experience . Am J Ophthalmol 1981;13:941-948. 5. Cowden JW, Bores LD: A clinical investigation of the surgical correction of myopia by the method of Fyodorov . Ophthalmology 1981;88:737-741.Crossref 6. Rowsey JJ, Balyeat HD: Preliminary results and complications of radial keratotomy . Am J Ophthalmol 1982;93:437-455. 7. Gelender H, Flynn HW, Mendelbaum S: Bacterial endophthalmitis resulting from radial keratotomy . Am J Ophthalmol 1982;93:323-326. 8. Katz HR, Duffin RM, Glasser DB, et al: Complications of contact lens wear after radial keratotomy in an animal model . Am J Ophthalmol 1982;94:377-382. 9. Stainer GA, Shaw EL, Binder PS, et al: Histopathology of a case of radial keratotomy . Arch Ophthalmol 1982;100:1473-1477.Crossref 10. Yamaguchi T, Polack FM, Valenti J, et al: Endothelial damage after anterior radial keratotomy: An electron microscope study of rabbit cornea . Arch Ophthalmol 1981;99:2151-2158.Crossref 11. Yamaguchi T, Kaufman HE, Fukushima A, et al: Histologic and electron microscopic assessment of endothelial damage produced by anterior radial keratotomy in the monkey cornea . Am J Ophthalmol 1981;92:313-327. 12. Waring GO, Moffitt SD, Gelender H, et al: Rationale for and design of the National Eye Institute prospective evaluation of radial keratotomy (PERK) study . Ophthalmology 1983;90:40-58.Crossref 13. Roberts CW, Hanninen L, Kenyon KR: Direct correlation of specular microscopy and electron microscopy in human and experimental corneal pathology . Invest Ophthalmol Vis Sci 1982;22( (suppl) ):29. 14. Krachmer JH, Schnitzer JI, Fratkin J: Cornea pseudoguttata: A clinical and histopathologic description of endothelial cell edema . Arch Ophthalmol 1981;99:1377-1381.Crossref 15. Rao GN, Shaw EL, Arthur E, et al: Morphological appearance of the healing corneal endothelium . Arch Ophthalmol 1978;96:2027-2030.Crossref 16. Cowden JW, Sultana M: Corneal endothelial cell density following radial keratotomy . Invest Ophthalmol Vis Sci 1982;22( (suppl) ):30. 17. Jester JV, Steel D, Salz J, et al: Radial keratotomy in non-human primate eye . Am J Ophthalmol 1981;92:153-171. 18. Smith RE, Jester JV, Arthur JB, et al: Endothelial studies following radial keratotomy in primate eyes . Invest Ophthalmol Vis Sci 1982;22( (suppl) ):240. 19. Bourne WM, Kaufman HE: Specular microscopy of human corneal endothelium in vivo . Am J Ophthalmol 1976;81:319-323. 20. Alvarado J, Murphy C, Polansky J, et al: Age-related changes in trabecular meshwork cellularity . Invest Ophthalmol Vis Sci 1981;21:714-727. 21. Bourne WM, Waller RR, Liesegang TJ, et al: Corneal trauma in intracapsular and extracapsular cataract extraction with lens implantation . Arch Ophthalmol 1981;99:1375-1376.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Endothelial Damage in Monkeys After Radial Keratotomy Performed With a Diamond Blade

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Publisher
American Medical Association
Copyright
Copyright © 1984 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1984.01040030613033
Publisher site
See Article on Publisher Site

Abstract

Abstract • Anterior radial keratotomy was performed with a diamond blade in ten rhesus monkey eyes. Results were compared with those of a previous study in which a metal blade was used. Histologic assessment showed endothelial degeneration, but fewer edematous endothelial cells, than in the previous study. Specular microscopy demonstrated statistically significant endothelial cell losses (7.99%), when preoperative and threemonth postoperative values were compared. Autoradiography showed little cell division in the endothelial cell layer. Cell loss seemed to be repaired mainly by the spreading of neighboring cells. Endothelial cell division is also limited in humans, and the cumulative loss of cells due to surgical trauma combined with continuous damage-related losses and later agerelated reductions in cell numbers could produce corneal decompensation in some patients years after radial keratotomy. References 1. Fyodorov SN, Durnev VV: Operation of dosaged dissection of corneal circular ligament in cases of myopia of mild degree . Ann Ophthalmol 1979;11:1885-1890. 2. Reddy PS, Reddy PR: Anterior keratotomy . Ophthalmic Surg 1980;11:765-767. 3. Hoffer KJ, Darian JJ, Pettit TH, et al: UCLA clinical trial of radial keratotomy . Ophthalmology 1981;88:729-736.Crossref 4. Bores LD, Myer W, Cowden J: Radial keratotomy; An analysis of the American experience . Am J Ophthalmol 1981;13:941-948. 5. Cowden JW, Bores LD: A clinical investigation of the surgical correction of myopia by the method of Fyodorov . Ophthalmology 1981;88:737-741.Crossref 6. Rowsey JJ, Balyeat HD: Preliminary results and complications of radial keratotomy . Am J Ophthalmol 1982;93:437-455. 7. Gelender H, Flynn HW, Mendelbaum S: Bacterial endophthalmitis resulting from radial keratotomy . Am J Ophthalmol 1982;93:323-326. 8. Katz HR, Duffin RM, Glasser DB, et al: Complications of contact lens wear after radial keratotomy in an animal model . Am J Ophthalmol 1982;94:377-382. 9. Stainer GA, Shaw EL, Binder PS, et al: Histopathology of a case of radial keratotomy . Arch Ophthalmol 1982;100:1473-1477.Crossref 10. Yamaguchi T, Polack FM, Valenti J, et al: Endothelial damage after anterior radial keratotomy: An electron microscope study of rabbit cornea . Arch Ophthalmol 1981;99:2151-2158.Crossref 11. Yamaguchi T, Kaufman HE, Fukushima A, et al: Histologic and electron microscopic assessment of endothelial damage produced by anterior radial keratotomy in the monkey cornea . Am J Ophthalmol 1981;92:313-327. 12. Waring GO, Moffitt SD, Gelender H, et al: Rationale for and design of the National Eye Institute prospective evaluation of radial keratotomy (PERK) study . Ophthalmology 1983;90:40-58.Crossref 13. Roberts CW, Hanninen L, Kenyon KR: Direct correlation of specular microscopy and electron microscopy in human and experimental corneal pathology . Invest Ophthalmol Vis Sci 1982;22( (suppl) ):29. 14. Krachmer JH, Schnitzer JI, Fratkin J: Cornea pseudoguttata: A clinical and histopathologic description of endothelial cell edema . Arch Ophthalmol 1981;99:1377-1381.Crossref 15. Rao GN, Shaw EL, Arthur E, et al: Morphological appearance of the healing corneal endothelium . Arch Ophthalmol 1978;96:2027-2030.Crossref 16. Cowden JW, Sultana M: Corneal endothelial cell density following radial keratotomy . Invest Ophthalmol Vis Sci 1982;22( (suppl) ):30. 17. Jester JV, Steel D, Salz J, et al: Radial keratotomy in non-human primate eye . Am J Ophthalmol 1981;92:153-171. 18. Smith RE, Jester JV, Arthur JB, et al: Endothelial studies following radial keratotomy in primate eyes . Invest Ophthalmol Vis Sci 1982;22( (suppl) ):240. 19. Bourne WM, Kaufman HE: Specular microscopy of human corneal endothelium in vivo . Am J Ophthalmol 1976;81:319-323. 20. Alvarado J, Murphy C, Polansky J, et al: Age-related changes in trabecular meshwork cellularity . Invest Ophthalmol Vis Sci 1981;21:714-727. 21. Bourne WM, Waller RR, Liesegang TJ, et al: Corneal trauma in intracapsular and extracapsular cataract extraction with lens implantation . Arch Ophthalmol 1981;99:1375-1376.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: May 1, 1984

References