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Electron Microscopical and Biochemical Analysis of Colloid Milium

Electron Microscopical and Biochemical Analysis of Colloid Milium Abstract Two new cases of colloid milium were studied by several methods to examine if collagen is the source of colloid. Woundhealing experiments in the lesion revealed that colloid is reproduced de novo with possible interaction with epidermal cells. Unfixed, native colloid did not contain collagen and salt extraction did not alter the ultrastructure of colloid. Tissue culture of colloid fibroblasts produced colloid-like substance. These results suggest that colloid is not a degeneration product of collagen. Disc electrophoresis of extracted colloid produced a distinct band, which is absent in the normal skin control. Amino acid analysis of colloid suggests that colloid may contain the recently described class of glycoprotein, called "structural glycoprotein" that is not related to collagen because it does not contain hydroxyproline and hydroxylysine and contains only a small amount of glucose. These biochemical data support the conclusion reached in electron microscopical studies. References 1. Hashimoto K, Miller F. Bereston ES: Colloid milium: Histochemical and electron microscopic studies . Arch Dermatol 105:684-694, 1972.Crossref 2. Hashimoto K, Onn LLY: Lichen amyloidosus: Electron microscopic study of a typical case and a review . Arch Dermatol 104:648-667, 1971.Crossref 3. Brownstein MH, Hashimoto K: Macular amyloidosis . Arch Dermatol 106:50-57, 1972.Crossref 4. Katzman RL: "Structural glycoprotein'' from Metridium dianthus connective tissue . Life Sciences ( (I) ) 11:131, 1972.Crossref 5. Reynolds ES: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy . J Cell Biol 17:208-212, 1963.Crossref 6. Weber K, Osborn M: The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis . J Biol Chem 244:4406, 1969. 7. Wolff I, Fuchswans W, Weiser M, et al: Acidic structural proteins of connective tissue . Eur J Biochem 20:426-431, 1971.Crossref 8. Kang AH: Studies on the location of intermolecular cross-links in collagen . Biochemistry 11:1828-1835, 1972.Crossref 9. Katzman RL, Oronsky AL: Evidence for a covalent linkage between heteropolysaccharide and an hydroxyproline-containing peptide from Metridium dianthus connective tissue . J Biol Chem 246:5107-5112, 1971. 10. Timple R, Wolff I, Weiser M: A new class of structural proteins from connective tissue. Biochem Biophys Acta 168:168-170, 1968. 11. Katzman RL, Kang AH, Dixit SN: The isolation and characterization of bovine corneal collagen . Biochem Biophys Acta 336:367-369, 1974. 12. Hashimoto K, Brownstein MH: Amyloidogenesis in healing wound: Electron microscopic studies of biopsied wounds in macular amyloidosis . Am J Pathol 68:371-380, 1972. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Electron Microscopical and Biochemical Analysis of Colloid Milium

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Publisher
American Medical Association
Copyright
Copyright © 1975 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1975.01630130051005
Publisher site
See Article on Publisher Site

Abstract

Abstract Two new cases of colloid milium were studied by several methods to examine if collagen is the source of colloid. Woundhealing experiments in the lesion revealed that colloid is reproduced de novo with possible interaction with epidermal cells. Unfixed, native colloid did not contain collagen and salt extraction did not alter the ultrastructure of colloid. Tissue culture of colloid fibroblasts produced colloid-like substance. These results suggest that colloid is not a degeneration product of collagen. Disc electrophoresis of extracted colloid produced a distinct band, which is absent in the normal skin control. Amino acid analysis of colloid suggests that colloid may contain the recently described class of glycoprotein, called "structural glycoprotein" that is not related to collagen because it does not contain hydroxyproline and hydroxylysine and contains only a small amount of glucose. These biochemical data support the conclusion reached in electron microscopical studies. References 1. Hashimoto K, Miller F. Bereston ES: Colloid milium: Histochemical and electron microscopic studies . Arch Dermatol 105:684-694, 1972.Crossref 2. Hashimoto K, Onn LLY: Lichen amyloidosus: Electron microscopic study of a typical case and a review . Arch Dermatol 104:648-667, 1971.Crossref 3. Brownstein MH, Hashimoto K: Macular amyloidosis . Arch Dermatol 106:50-57, 1972.Crossref 4. Katzman RL: "Structural glycoprotein'' from Metridium dianthus connective tissue . Life Sciences ( (I) ) 11:131, 1972.Crossref 5. Reynolds ES: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy . J Cell Biol 17:208-212, 1963.Crossref 6. Weber K, Osborn M: The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis . J Biol Chem 244:4406, 1969. 7. Wolff I, Fuchswans W, Weiser M, et al: Acidic structural proteins of connective tissue . Eur J Biochem 20:426-431, 1971.Crossref 8. Kang AH: Studies on the location of intermolecular cross-links in collagen . Biochemistry 11:1828-1835, 1972.Crossref 9. Katzman RL, Oronsky AL: Evidence for a covalent linkage between heteropolysaccharide and an hydroxyproline-containing peptide from Metridium dianthus connective tissue . J Biol Chem 246:5107-5112, 1971. 10. Timple R, Wolff I, Weiser M: A new class of structural proteins from connective tissue. Biochem Biophys Acta 168:168-170, 1968. 11. Katzman RL, Kang AH, Dixit SN: The isolation and characterization of bovine corneal collagen . Biochem Biophys Acta 336:367-369, 1974. 12. Hashimoto K, Brownstein MH: Amyloidogenesis in healing wound: Electron microscopic studies of biopsied wounds in macular amyloidosis . Am J Pathol 68:371-380, 1972.

Journal

Archives of DermatologyAmerican Medical Association

Published: Jan 1, 1975

References