Abstract • Our previous studies of the effects of the in vivo administration of anti-Ia (anti—class II major histocompatibility antigens) antibodies in mice demonstrated that, although the antibodies bind to epidermal Langerhans' cells, they do not affect their antigen-presenting capacity in vitro. In this study we investigated the effects of the in vivo administration of these antibodies on the induction and expression of contact sensitivity. We found that the antibodies inhibit the induction of contact sensitivity significantly and affect the elicitation phase to a much lesser extent. The inhibition is short-lived and probably not attributable to the induction of suppressor cells. (Arch Dermatol 1989;125:280-284) References 1. Rowden G: The Langerhans cell . Crit Rev Immunol 1981;3:94-180. 2. Stingl G, Tamaki K, Katz SI: Origin and function of epidermal Langerhans cell . Immunol Rev 1980;53:149-174.Crossref 3. Davis DAL, Staines NA: A cardinal role for I-region antigens (Ia) in immunological enhancement and the clinical implications . Transplant Rev 1976;30:18-39. 4. Prudhomme GJ, Sohn U, Delovitch TL: The role of H-2 and Ia antigens in graft-vs-host reactions (GVHR): Presence of host alloantigens on donor cells after GVHR and suppression of GVHR with an anti-Ia antiserum against host Ia antigens . J Exp Med 1979;149:137-149.Crossref 5. Perry LL, Williams IR: Regulation of transplantation immunity in vivo by monoclonal antibodies recognizing host class II restriction elements: I. Genetics and specificity of anti-Ia immunotherapy in murine skin allograft recipients . J Immunol 1985;134:2935-2941. 6. Perry LL, Greene MI: Conversion of immunity to suppression by in vivo administration of I-A subregion-specific antibodies . J Exp Med 1982;156:480-492.Crossref 7. Hodes RJ, Hatcock KS, Singer A: Major histocompatibility complex restricted self recognition: A monoclonal anti—I-Ak reagent blocks helper T cell recognition of self major histocompatibility complex determinants . J Exp Med 1980;152:1779-1794.Crossref 8. Longo DL, Schwartz RH: Inhibition of antigen-induced proliferation of T cells from radiation-induced bone marrow chimeras by monoclonal antibody against a Ia determinant on the antigen-presenting cell . Proc Natl Acad Sci USA 1981;78:514-518.Crossref 9. Nepom JT, Benacerraf B, Germain RN: Analysis of Ir gene function by using monoclonal antibodies: Independent regulation of GAT and GLPhe T cell responses by I-A and I-E subregion products on a single accessory cell population . J Immunol 1981;127:31-34. 10. Kruisbeek AM, Titus JA, Stephany DA, et al: In vivo treatment with monoclonal anti—IA antibodies: Disappearance of splenic antigen-presenting cell function concomitant with modulation of splenic cell surface I-A and I-E antigens . J Immunol 1985;134:3605-3614. 11. Aberer W, Kruisbeek AM, Shimada S, et al: In vivo treatment with anti—IA antibodies: Differential effects of Ia antigens and antigen-presenting cell function of spleen cells and epidermal Langerhans cells . J Immunol 1986;136:830-836. 12. Perry LL, Dorf ME, Benacerraf B, et al: Regulation of immune response to tumor antigen: Interference with syngeneic tumor immunity by anti—I A alloantisera . Proc Natl Acad Sci USA 1979;76:920-924.Crossref 13. Perry LL, Dorf ME, Back BA, et al: Mechanisms of regulation of cell-mediated immunity: Anti—I A alloantisera interfere with induction and expression of T-cell-mediated immunity to cell bound antigen in vivo . Clin Immunol Immunopathol 1980;15:279-292.Crossref 14. Sprent J: Effects of blocking helper T cell induction in vivo with anti-Ia antibodies: Possible role of I-A/E hybrid molecules as restriction elements . J Exp Med 1980;152:996-1010.Crossref 15. Rosenbaum JT, Adelman NE, McDevitt HO: In vivo effects of antibodies to immune response gene products: I. Haplotype-specific suppression of humoral immune responses with a monoclonal anti-IA . J Exp Med 1981;154:1694-1702.Crossref 16. Steinman L, Rosenbaum RT, Sriram S, et al: In vivo effects of antibodies to immune response gene products: Prevention of experimental allergic encephalitis . Proc Natl Acad Sci USA 1981;78:7111-7114.Crossref 17. Tamaki K, Stingl G, Gullino M, et al: Ia antigens in mouse skin are predominantly expressed on Langerhans cells . J Immunol 1978;123:784-789.
Archives of Dermatology – American Medical Association
Published: Feb 1, 1989