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Early Diagnosis of Bacteremia by Buffy-Coat Examinations

Early Diagnosis of Bacteremia by Buffy-Coat Examinations Abstract A smear of leukocytes from the buffy-coat layer was obtained when peripheral blood specimens were taken for culture. Fourteen of 135 blood cultures were positive, and five of the 14, from three patients, showed Gram-positive intracellular organisms on Gram stain of the buffy-coat layer. Grampositive cocci were seen in two of three patients with staphylococcal bacteremia and Gram-positive rods in one patient with Clostridium perfringens bacteremia. These two cases illustrate the diagnostic role of the buffy-coat smear and its use in the early diagnosis of bacteremia. References 1. MacGregor RR, Beaty HN: Evaluation of positive blood cultures: Guidelines for the early differentiation of contaminated from valid cultures. Arch Intern Med 130:84-87, 1972.Crossref 2. Davidsohn I, Nelson DA: The blood , in Davidsohn I, Henry JB (eds): Clinical Diagnosis by Laboratory Methods . Philadelphia, WB Saunders, 1969, pp 161-166. 3. Paine TF: Gram staining without the clock. N Engl J Med 268:941, 1963.Crossref 4. Humphrey AA: Use of the buffy layer in the rapid diagnosis of septicemia. Am J Clin Pathol 14:358-362, 1944. 5. McCabe WR, LaPorte JJ: Intracellular bacteria in the peripheral blood in staphylococcal bacteremia. Ann Intern Med 57:141-143, 1962.Crossref 6. Falkinburg LW, et al: Phagocytosis of staphylococci seen in peripheral blood smear. JAMA 182:868-869, 1962.Crossref 7. Smith H: Leukocytes containing bacteria in plain blood films from patients with septicaemia. Australas Ann Med 15:210-221, 1966. 8. Boger WP: Fulminating meningococcemia: Demonstration of intracellular and extracellular meningococci in direct smears of the blood. N Engl J Med 231:385-387, 1944.Crossref 9. Falkinburg LW, Troppoli AV: The early diagnosis of the Waterhouse-Fridericksen syndrome. Am Practitioner 8:1047-1049, 1957. 10. McLean S, Caffey J: Endemic purpuric meningococcus bacteremia in early life: The diagnostic value of smears from the purpuric lesions. Am J Dis Child 42:1053-1074, 1931.Crossref 11. Portnoy J, et al: Candida blastospores and pseudohyphae in blood smears. N Engl J Med 285:1010-1011, 1971.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Internal Medicine American Medical Association

Early Diagnosis of Bacteremia by Buffy-Coat Examinations

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Publisher
American Medical Association
Copyright
Copyright © 1973 American Medical Association. All Rights Reserved.
ISSN
0003-9926
eISSN
1538-3679
DOI
10.1001/archinte.1973.03650110029006
Publisher site
See Article on Publisher Site

Abstract

Abstract A smear of leukocytes from the buffy-coat layer was obtained when peripheral blood specimens were taken for culture. Fourteen of 135 blood cultures were positive, and five of the 14, from three patients, showed Gram-positive intracellular organisms on Gram stain of the buffy-coat layer. Grampositive cocci were seen in two of three patients with staphylococcal bacteremia and Gram-positive rods in one patient with Clostridium perfringens bacteremia. These two cases illustrate the diagnostic role of the buffy-coat smear and its use in the early diagnosis of bacteremia. References 1. MacGregor RR, Beaty HN: Evaluation of positive blood cultures: Guidelines for the early differentiation of contaminated from valid cultures. Arch Intern Med 130:84-87, 1972.Crossref 2. Davidsohn I, Nelson DA: The blood , in Davidsohn I, Henry JB (eds): Clinical Diagnosis by Laboratory Methods . Philadelphia, WB Saunders, 1969, pp 161-166. 3. Paine TF: Gram staining without the clock. N Engl J Med 268:941, 1963.Crossref 4. Humphrey AA: Use of the buffy layer in the rapid diagnosis of septicemia. Am J Clin Pathol 14:358-362, 1944. 5. McCabe WR, LaPorte JJ: Intracellular bacteria in the peripheral blood in staphylococcal bacteremia. Ann Intern Med 57:141-143, 1962.Crossref 6. Falkinburg LW, et al: Phagocytosis of staphylococci seen in peripheral blood smear. JAMA 182:868-869, 1962.Crossref 7. Smith H: Leukocytes containing bacteria in plain blood films from patients with septicaemia. Australas Ann Med 15:210-221, 1966. 8. Boger WP: Fulminating meningococcemia: Demonstration of intracellular and extracellular meningococci in direct smears of the blood. N Engl J Med 231:385-387, 1944.Crossref 9. Falkinburg LW, Troppoli AV: The early diagnosis of the Waterhouse-Fridericksen syndrome. Am Practitioner 8:1047-1049, 1957. 10. McLean S, Caffey J: Endemic purpuric meningococcus bacteremia in early life: The diagnostic value of smears from the purpuric lesions. Am J Dis Child 42:1053-1074, 1931.Crossref 11. Portnoy J, et al: Candida blastospores and pseudohyphae in blood smears. N Engl J Med 285:1010-1011, 1971.Crossref

Journal

Archives of Internal MedicineAmerican Medical Association

Published: Nov 1, 1973

References