Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Dye-Binding Capacity of Serum Albumin in Hemolytic Disease of the Newborn

Dye-Binding Capacity of Serum Albumin in Hemolytic Disease of the Newborn Abstract In recent years considerable evidence has been reported in support of the theory that bilirubin is toxic to the newborn brain (bilirubin encephalopathy). It has been further suggested that perhaps free bilirubin, that which is not bound to albumin, is the toxic substance.1 Utilizing this information, studies were done in newborn puppies which suggested a protective action for albumin against bilirubin toxicity.2 It therefore seemed advisable to determine the binding capacity of albumin in the newborn, particularly in the infant with hemolytic disease. A reduction in the capacity of serum albumin to bind anionic dyes has been reported in patients with cancer,3 severe kidney and liver disease, infections,4 and in pregnancy.5 Blondheim6 found that the amount of phenolsulfonphthalein bound by serum was related to the albumin concentration in excess of a critical concentration of 1.5 gm.%. He concluded that dye-binding in diseased states was References 1. Sass-Kortsak, A., editor, Kernicterus , Toronto, University of Toronto Press, 1961. 2. Bowen, W. R.; Porter, E., and Waters, W. J.: The Protective Action of Albumin in Bilirubin Toxicity in Newborn Puppies , A.M.A. J. Dis. Child. 98:568, 1959. 3. Huggins, C.; Jensen, E. V.; Player, M. A., and Hospelhorn, V. D.: The Binding of Phenolsulfonephthalcin by Serum and by Albumin Isolated from Serum in Cancer , Cancer Res. 9:753, 1949. 4. Westphal, U., and Gedigk, P.: Azorubin-Binding Capacity of Normal and Pathological Sera , Proc. Soc. Exp. Biol. Med. 76:838, 1951.Crossref 5. Miller, G. H.; Davis, M. E.; King, A. G., and Huggins, C. B.: Serum Proteins in Pregnancy: Thermal Coagulation and the Binding of Anions , J. Lab. Clin. Med. 37:538, 1951. 6. Blondheim, S. H.: The Relationship Between the Albumin Concentration of Serum and Its Dye-Binding Capacity , J. Lab. Clin. Med. 45:740, 1955. 7. Waters, W. J.: The Prevention of Bilirubin Encephalopathy , J. Pediat. 52:559, 1958.Crossref 8. Klotz, I. M.: Spectrophotometric Investigations of the Interactions of Proteins with Organic Anions , J. Amer. Chem. Soc. 68:2299, 1946.Crossref 9. Klotz, I. M.; Triwush, H., and Walker, F. M.: The Binding of Organic Ions by Proteins: Competition Phenomena and Denaturation Effects , J. Amer. Chem. Soc. 70:2935, 1948.Crossref 10. Odell, G. B.: Studies in Kernicterus: 1. The Protein Binding of Bilirubin , J. Clin. Invest. 38: 823, 1959.Crossref 11. Odell, G. B.: The Dissociation of Bilirubin from Albumin and Its Clinical Implications , J. Pediat. 55:268, 1959.Crossref 12. Westphal, U.; Stets, J. F., and Priest, S. G.: Influence of Fatty Acids and Related Anions on the Azorubin-Binding Capacity of Serum Albumin , Arch. Biochem. 43:463, 1953.Crossref 13. Waters, W. J.: The Origin of Jaundice in the Newborn , Amer. J. Dis. Child. 100:785, 1960. 14. Martin, N. H.: Preparation and Properties of Serum and Plasma Proteins: XXI. Interactions with Bilirubin , J. Amer. Chem. Soc. 71:1230, 1949.Crossref 15. Kitchen, W. H.; Krieger, V. I., and Smith, M. A.: Human Albumin in Exchange Transfusion , J. Pediat. 57:876, 1960.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Dye-Binding Capacity of Serum Albumin in Hemolytic Disease of the Newborn

Loading next page...
 
/lp/american-medical-association/dye-binding-capacity-of-serum-albumin-in-hemolytic-disease-of-the-EGrkYk2OoP

References (15)

Publisher
American Medical Association
Copyright
Copyright © 1961 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1961.02080010809004
Publisher site
See Article on Publisher Site

Abstract

Abstract In recent years considerable evidence has been reported in support of the theory that bilirubin is toxic to the newborn brain (bilirubin encephalopathy). It has been further suggested that perhaps free bilirubin, that which is not bound to albumin, is the toxic substance.1 Utilizing this information, studies were done in newborn puppies which suggested a protective action for albumin against bilirubin toxicity.2 It therefore seemed advisable to determine the binding capacity of albumin in the newborn, particularly in the infant with hemolytic disease. A reduction in the capacity of serum albumin to bind anionic dyes has been reported in patients with cancer,3 severe kidney and liver disease, infections,4 and in pregnancy.5 Blondheim6 found that the amount of phenolsulfonphthalein bound by serum was related to the albumin concentration in excess of a critical concentration of 1.5 gm.%. He concluded that dye-binding in diseased states was References 1. Sass-Kortsak, A., editor, Kernicterus , Toronto, University of Toronto Press, 1961. 2. Bowen, W. R.; Porter, E., and Waters, W. J.: The Protective Action of Albumin in Bilirubin Toxicity in Newborn Puppies , A.M.A. J. Dis. Child. 98:568, 1959. 3. Huggins, C.; Jensen, E. V.; Player, M. A., and Hospelhorn, V. D.: The Binding of Phenolsulfonephthalcin by Serum and by Albumin Isolated from Serum in Cancer , Cancer Res. 9:753, 1949. 4. Westphal, U., and Gedigk, P.: Azorubin-Binding Capacity of Normal and Pathological Sera , Proc. Soc. Exp. Biol. Med. 76:838, 1951.Crossref 5. Miller, G. H.; Davis, M. E.; King, A. G., and Huggins, C. B.: Serum Proteins in Pregnancy: Thermal Coagulation and the Binding of Anions , J. Lab. Clin. Med. 37:538, 1951. 6. Blondheim, S. H.: The Relationship Between the Albumin Concentration of Serum and Its Dye-Binding Capacity , J. Lab. Clin. Med. 45:740, 1955. 7. Waters, W. J.: The Prevention of Bilirubin Encephalopathy , J. Pediat. 52:559, 1958.Crossref 8. Klotz, I. M.: Spectrophotometric Investigations of the Interactions of Proteins with Organic Anions , J. Amer. Chem. Soc. 68:2299, 1946.Crossref 9. Klotz, I. M.; Triwush, H., and Walker, F. M.: The Binding of Organic Ions by Proteins: Competition Phenomena and Denaturation Effects , J. Amer. Chem. Soc. 70:2935, 1948.Crossref 10. Odell, G. B.: Studies in Kernicterus: 1. The Protein Binding of Bilirubin , J. Clin. Invest. 38: 823, 1959.Crossref 11. Odell, G. B.: The Dissociation of Bilirubin from Albumin and Its Clinical Implications , J. Pediat. 55:268, 1959.Crossref 12. Westphal, U.; Stets, J. F., and Priest, S. G.: Influence of Fatty Acids and Related Anions on the Azorubin-Binding Capacity of Serum Albumin , Arch. Biochem. 43:463, 1953.Crossref 13. Waters, W. J.: The Origin of Jaundice in the Newborn , Amer. J. Dis. Child. 100:785, 1960. 14. Martin, N. H.: Preparation and Properties of Serum and Plasma Proteins: XXI. Interactions with Bilirubin , J. Amer. Chem. Soc. 71:1230, 1949.Crossref 15. Kitchen, W. H.; Krieger, V. I., and Smith, M. A.: Human Albumin in Exchange Transfusion , J. Pediat. 57:876, 1960.Crossref

Journal

American Journal of Diseases of ChildrenAmerican Medical Association

Published: Dec 1, 1961

There are no references for this article.