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Development of a Radioimmunoassay for Gastrin

Development of a Radioimmunoassay for Gastrin Abstract Early studies on the physiology of gastrin were dependent upon the observed secretory response, but direct measurement has been made possible by the recent development of a radioimmunoassay by McGuigan.1 Because gastrin has functional and structural similarities with pancreozymin-cholecystokinin (PZ-CCK), with synthetic pentagastrin, and with caerulein,2 it is desirable that an assay be highly specific for gastrin. In this report we describe the development of a specific radioimmunoassay in which the antibodies were made with unconjugated gastrin used as an antigen. The antibodies have proved to be specific for gastrin and show only minimal cross-reaction with related substances. Gastrin has been measured in antral extracts and the serum and tumor extracts of patients with the Zollinger-Ellison syndrome. Materials and Methods Antibodies were prepared by immunizing with hog gastrin and synthetic human gastrin 1. The hog gastrin was prepared in this laboratory by the method of Gregory and Tracy. References 1. McGuigan, J.E.: Immunochemical Studies With Synthetic Human Gastrin , Gastroenterology 54:1005-1011 ( (June) ) 1968. 2. Thompson, J.C.: Gastrin and Gastric Secretion , Ann Rev Med 20:291-314 ( (May) ) 1969.Crossref 3. Gregory, R.A., and Tracy, H.J.: Constitution and Properties of Two Gastrins Extracted From Hog Antral Mucosa , Gut 5:103-114 ( (April) ) 1964.Crossref 4. Greenwood, F.C.; Hunter, W.M.; and Glover, J.S.: The Preparation of I131-Labeled Human Growth Hormone of High Specific Radioactivity , Biochem J 89:114-123 ( (Oct) ) 1963. 5. Odell, W.D.; Rayford, P.L.; and Ross, G.T.: Simplified Partially Automated Method for Radioimmunoassay of Human Thyroid-Stimulating, Growth, Luteinizing, and Follicle Stimulating Hormones , J Lab Clin Med 70:973-980 ( (Dec) ) 1967. 6. Odell, W.D., et al: Radioimmunoassay for Human Follicle-Stimulating Hormone , J Clin Invest 47:2551-2562 ( (Dec) ) 1968.Crossref 7. Thompson, J.C., et al: Zollinger-Ellison Syndrome in a Patient With Multiple Carcinoid-Islet Cell Tumors of the Duodenum: Effect of a Tumor Extract on Isolated Gastric Mucosa , Amer J Surg 115:177-184 ( (Feb) ) 1968.Crossref 8. Stremple, J.F., et al: Antibodies to Synthetic Human Gastrin , Lancet 2:1180-1182 ( (Dec) ) 1967.Crossref 9. Schneider, D.R., et al: Gastrin Antibodies: Induction, Demonstration and Specificity , Science 156:391-392 ( (April) ) 1967.Crossref 10. Mutt, V., and Jorpes, J.E.: Isolation of Aspartyl-Phenylalanine Amide From Cholecystokinin-Pancreozymin , Biochem Biophys Res Commun 26:392-397 ( (Feb) ) 1967.Crossref 11. Mutt, V., and Jorpes, J.E.: Chemistry and Physiology of Cholecystokinin-Pancreozymin , Proc Int Union Physiol Sci 6:193, 1968. 12. Charters, A.C., et al: Common Properties of Gastrin and Pancreozymin-Cholecystokinin, Gastroenterology, to be published. 13. Odell, W.D., et al: Radioimmunoassay for Human Gastrin Using Unconjugated Gastrin as an Antigen , J Clin Endocr 28:1840-1842 ( (Dec) ) 1968.Crossref 14. Zollinger, R.M., and Ellison, E.H.: Primary Peptic Ulcerations of the Jejunum Associated With Islet Cell Tumors of the Pancreas , Ann Surg 142:709-728 ( (Oct) ) 1955.Crossref 15. Gregory, R.A., et al: Nature of the Gastric Secretagogue in Zollinger-Ellison Tumors , Lancet 2:543-544 ( (Sept) ) 1967.Crossref 16. McGuigan, J.E., and Trudeau, W.L.: Immunochemical Measurement of Elevated Levels of Gastrin in the Serum of Patients With Pancreatic Tumors of the Zollinger-Ellison Variety , New Eng J Med 278:1308-1313 ( (June) ) 1968.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

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Publisher
American Medical Association
Copyright
Copyright © 1969 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1969.01340150069013
Publisher site
See Article on Publisher Site

Abstract

Abstract Early studies on the physiology of gastrin were dependent upon the observed secretory response, but direct measurement has been made possible by the recent development of a radioimmunoassay by McGuigan.1 Because gastrin has functional and structural similarities with pancreozymin-cholecystokinin (PZ-CCK), with synthetic pentagastrin, and with caerulein,2 it is desirable that an assay be highly specific for gastrin. In this report we describe the development of a specific radioimmunoassay in which the antibodies were made with unconjugated gastrin used as an antigen. The antibodies have proved to be specific for gastrin and show only minimal cross-reaction with related substances. Gastrin has been measured in antral extracts and the serum and tumor extracts of patients with the Zollinger-Ellison syndrome. Materials and Methods Antibodies were prepared by immunizing with hog gastrin and synthetic human gastrin 1. The hog gastrin was prepared in this laboratory by the method of Gregory and Tracy. References 1. McGuigan, J.E.: Immunochemical Studies With Synthetic Human Gastrin , Gastroenterology 54:1005-1011 ( (June) ) 1968. 2. Thompson, J.C.: Gastrin and Gastric Secretion , Ann Rev Med 20:291-314 ( (May) ) 1969.Crossref 3. Gregory, R.A., and Tracy, H.J.: Constitution and Properties of Two Gastrins Extracted From Hog Antral Mucosa , Gut 5:103-114 ( (April) ) 1964.Crossref 4. Greenwood, F.C.; Hunter, W.M.; and Glover, J.S.: The Preparation of I131-Labeled Human Growth Hormone of High Specific Radioactivity , Biochem J 89:114-123 ( (Oct) ) 1963. 5. Odell, W.D.; Rayford, P.L.; and Ross, G.T.: Simplified Partially Automated Method for Radioimmunoassay of Human Thyroid-Stimulating, Growth, Luteinizing, and Follicle Stimulating Hormones , J Lab Clin Med 70:973-980 ( (Dec) ) 1967. 6. Odell, W.D., et al: Radioimmunoassay for Human Follicle-Stimulating Hormone , J Clin Invest 47:2551-2562 ( (Dec) ) 1968.Crossref 7. Thompson, J.C., et al: Zollinger-Ellison Syndrome in a Patient With Multiple Carcinoid-Islet Cell Tumors of the Duodenum: Effect of a Tumor Extract on Isolated Gastric Mucosa , Amer J Surg 115:177-184 ( (Feb) ) 1968.Crossref 8. Stremple, J.F., et al: Antibodies to Synthetic Human Gastrin , Lancet 2:1180-1182 ( (Dec) ) 1967.Crossref 9. Schneider, D.R., et al: Gastrin Antibodies: Induction, Demonstration and Specificity , Science 156:391-392 ( (April) ) 1967.Crossref 10. Mutt, V., and Jorpes, J.E.: Isolation of Aspartyl-Phenylalanine Amide From Cholecystokinin-Pancreozymin , Biochem Biophys Res Commun 26:392-397 ( (Feb) ) 1967.Crossref 11. Mutt, V., and Jorpes, J.E.: Chemistry and Physiology of Cholecystokinin-Pancreozymin , Proc Int Union Physiol Sci 6:193, 1968. 12. Charters, A.C., et al: Common Properties of Gastrin and Pancreozymin-Cholecystokinin, Gastroenterology, to be published. 13. Odell, W.D., et al: Radioimmunoassay for Human Gastrin Using Unconjugated Gastrin as an Antigen , J Clin Endocr 28:1840-1842 ( (Dec) ) 1968.Crossref 14. Zollinger, R.M., and Ellison, E.H.: Primary Peptic Ulcerations of the Jejunum Associated With Islet Cell Tumors of the Pancreas , Ann Surg 142:709-728 ( (Oct) ) 1955.Crossref 15. Gregory, R.A., et al: Nature of the Gastric Secretagogue in Zollinger-Ellison Tumors , Lancet 2:543-544 ( (Sept) ) 1967.Crossref 16. McGuigan, J.E., and Trudeau, W.L.: Immunochemical Measurement of Elevated Levels of Gastrin in the Serum of Patients With Pancreatic Tumors of the Zollinger-Ellison Variety , New Eng J Med 278:1308-1313 ( (June) ) 1968.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Sep 1, 1969

References