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Detection of Endotoxin in Cerebrospinal and Joint Fluids by Limulus Assay

Detection of Endotoxin in Cerebrospinal and Joint Fluids by Limulus Assay Abstract Cerebrospinal fluid (CSF) and joint fluid from 81 patients were studied for the presence of endotoxin using the limulus assay. All fluids that grew Gram-negative organisms had a positive limulus test. However, one sample of CSF and one of joint fluid, both very purulent, that contained Gram-positive organisms also gave positive limulus assays. All culturally negative fluids had a negative limulus assay. Thus, a positive limulus assay with CSF or joint fluid is indicative of bacterial infection probably caused by Gram-negative organisms and can be an extremely useful diagnostic adjunct. (Arch Intern Med 137:55-56, 1977) References 1. Levin J, Poore TE, Zauber NP, et al: Detection of endotoxin in the blood of patients with sepsis due to Gram-negative bacteria . N Engl J Med 283:1313-1316, 1970.Crossref 2. Levin J, Poore TE, Young NS, et al: Gram negative sepsis: Detection of endotoxemia with the limulus test: With studies of associated changes in blood coagulation, serum lipids, and complement . Ann Intern Med 76:1-7, 1972.Crossref 3. Stumacher RS, Kovnat MS, McCabe WR: Limitations of the usefulness of the limulus assay for endotoxin . N Engl J Med 288:1261-1264, 1973.Crossref 4. Martinez GLA, Quintiliani R, Tilton RC: Clinical experience on the detection of endotoxemia with the limulus test . Infect Dis 127:102-105, 1973.Crossref 5. Feldman S, Pearson TA: The limulus test and Gram-negative bacillary sepsis . Am J Dis Child 128:172-174, 1974. 6. Elin RJ, Robinson RA, Levine AS, et al: Lack of clinical usefulness of the limulus test in the diagnosis of endotoxemia . N Engl J Med 272:725-731, 1962. 7. Nachum R, Lippey A, Siegel S: Rapid detection of Gram-negative bacterial meningitis by the limulus lysate test . N Engl J Med 289:931-934, 1973.Crossref 8. Ross S, Rodriguez W, Controni G, et al: Limulus lysate test for Gram-negative bacterial meningitis: Bedside application . JAMA 233:13661369, 1975.Crossref 9. Smith DH, Ingram DL, Smith AL, et al: Diagnosis and treatment: Bacterial meningitis . Pediatrics 52:586-600, 1973. 10. Coonrod JD, Rytel MW: Determination of etiology of bacterial meningitis by counter immunoelectrophoresis . Lancet 1:1154-1157, 1972.Crossref 11. Edwards EA, Muehl PM, Peckinpaugh RO: Diagnosis of bacterial meningitis by counter immunoelectrophoresis . J Lab Clin Med 80:449-454, 1972. 12. Levin J, Tomasulo PA, Oser RS: Detection of endotoxin in human blood and demonstration of an inhibitor . J Lab Clin Med 75:903-910, 1970. 13. Reinhold RB, Fine J: A technique for quantitative measurement of endotoxin in human plasma . Proc Soc Ex Biol Med 137:334-340, 1971.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Internal Medicine American Medical Association

Detection of Endotoxin in Cerebrospinal and Joint Fluids by Limulus Assay

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Publisher
American Medical Association
Copyright
Copyright © 1977 American Medical Association. All Rights Reserved.
ISSN
0003-9926
eISSN
1538-3679
DOI
10.1001/archinte.1977.03630130039010
Publisher site
See Article on Publisher Site

Abstract

Abstract Cerebrospinal fluid (CSF) and joint fluid from 81 patients were studied for the presence of endotoxin using the limulus assay. All fluids that grew Gram-negative organisms had a positive limulus test. However, one sample of CSF and one of joint fluid, both very purulent, that contained Gram-positive organisms also gave positive limulus assays. All culturally negative fluids had a negative limulus assay. Thus, a positive limulus assay with CSF or joint fluid is indicative of bacterial infection probably caused by Gram-negative organisms and can be an extremely useful diagnostic adjunct. (Arch Intern Med 137:55-56, 1977) References 1. Levin J, Poore TE, Zauber NP, et al: Detection of endotoxin in the blood of patients with sepsis due to Gram-negative bacteria . N Engl J Med 283:1313-1316, 1970.Crossref 2. Levin J, Poore TE, Young NS, et al: Gram negative sepsis: Detection of endotoxemia with the limulus test: With studies of associated changes in blood coagulation, serum lipids, and complement . Ann Intern Med 76:1-7, 1972.Crossref 3. Stumacher RS, Kovnat MS, McCabe WR: Limitations of the usefulness of the limulus assay for endotoxin . N Engl J Med 288:1261-1264, 1973.Crossref 4. Martinez GLA, Quintiliani R, Tilton RC: Clinical experience on the detection of endotoxemia with the limulus test . Infect Dis 127:102-105, 1973.Crossref 5. Feldman S, Pearson TA: The limulus test and Gram-negative bacillary sepsis . Am J Dis Child 128:172-174, 1974. 6. Elin RJ, Robinson RA, Levine AS, et al: Lack of clinical usefulness of the limulus test in the diagnosis of endotoxemia . N Engl J Med 272:725-731, 1962. 7. Nachum R, Lippey A, Siegel S: Rapid detection of Gram-negative bacterial meningitis by the limulus lysate test . N Engl J Med 289:931-934, 1973.Crossref 8. Ross S, Rodriguez W, Controni G, et al: Limulus lysate test for Gram-negative bacterial meningitis: Bedside application . JAMA 233:13661369, 1975.Crossref 9. Smith DH, Ingram DL, Smith AL, et al: Diagnosis and treatment: Bacterial meningitis . Pediatrics 52:586-600, 1973. 10. Coonrod JD, Rytel MW: Determination of etiology of bacterial meningitis by counter immunoelectrophoresis . Lancet 1:1154-1157, 1972.Crossref 11. Edwards EA, Muehl PM, Peckinpaugh RO: Diagnosis of bacterial meningitis by counter immunoelectrophoresis . J Lab Clin Med 80:449-454, 1972. 12. Levin J, Tomasulo PA, Oser RS: Detection of endotoxin in human blood and demonstration of an inhibitor . J Lab Clin Med 75:903-910, 1970. 13. Reinhold RB, Fine J: A technique for quantitative measurement of endotoxin in human plasma . Proc Soc Ex Biol Med 137:334-340, 1971.Crossref

Journal

Archives of Internal MedicineAmerican Medical Association

Published: Jan 1, 1977

References