Abstract • Short-term eye banking is based mainly on moist chamber and McCarey-Kaufman medium (M-K medium) preservation. BOth involve a controlled 4 C temperature for storage. Warming the cornea to room temperature, however, drastically affects the endothelial viability. On enzymatic staining and histological study, the M-K medium-stored rabbit corneas had more normal endothelium than did "moist chamber" eyes when storage was prolonged for seven days at room temperature. In human corneas that were kept at 4 C for 24 hours and then exposed to a temperature of 25 C, destruction of organelles had occurred by six hours and was increased by 12 hours. Corneas that were kept in M-K medium had relatively intact endothelium after four days, but cell disruption and vacuolation was present by the seventh day. The M-K medium, therefore, affords protection to tissue warmed to room temperature, where metabolic activity is resumed. References 1. McCarey BE, Kaufman HE: Improved corneal storage . Invest Ophthalmol 13:165-173, 1974. 2. Bigar F, Kaufman HE, McCarey B, et al: Improved corneal storage for penetrating keratoplasties in man . Am J Ophthalmol 79:115-120, 1975. 3. Robbins JE, Capella JA, Kaufman HE: A study of endothelium in keratoplasty and corneal preservation . Arch Ophthalmol 73:242-247, 1965.Crossref 4. Polack FM: Scanning electron microscopy of corneal graft rejection . Invest Ophthalmol 11:1-14, 1972. 5. McCarey BE, Sakimoto T, Bigar F: Ultrastructure of M-K and refrigerated moist chamber-stored corneas . Invest Ophthalmol 13:859-863, 1974. 6. Lindstrom RL, Doughman DJ, Van Horn DL, et al: Organ culture corneal storage at ambient room temperature . Arch Ophthalmol 95:869-878, 1977.Crossref
Archives of Ophthalmology – American Medical Association
Published: Jun 1, 1978
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