Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Corneal Storage at Room Temperature

Corneal Storage at Room Temperature Abstract • Short-term eye banking is based mainly on moist chamber and McCarey-Kaufman medium (M-K medium) preservation. BOth involve a controlled 4 C temperature for storage. Warming the cornea to room temperature, however, drastically affects the endothelial viability. On enzymatic staining and histological study, the M-K medium-stored rabbit corneas had more normal endothelium than did "moist chamber" eyes when storage was prolonged for seven days at room temperature. In human corneas that were kept at 4 C for 24 hours and then exposed to a temperature of 25 C, destruction of organelles had occurred by six hours and was increased by 12 hours. Corneas that were kept in M-K medium had relatively intact endothelium after four days, but cell disruption and vacuolation was present by the seventh day. The M-K medium, therefore, affords protection to tissue warmed to room temperature, where metabolic activity is resumed. References 1. McCarey BE, Kaufman HE: Improved corneal storage . Invest Ophthalmol 13:165-173, 1974. 2. Bigar F, Kaufman HE, McCarey B, et al: Improved corneal storage for penetrating keratoplasties in man . Am J Ophthalmol 79:115-120, 1975. 3. Robbins JE, Capella JA, Kaufman HE: A study of endothelium in keratoplasty and corneal preservation . Arch Ophthalmol 73:242-247, 1965.Crossref 4. Polack FM: Scanning electron microscopy of corneal graft rejection . Invest Ophthalmol 11:1-14, 1972. 5. McCarey BE, Sakimoto T, Bigar F: Ultrastructure of M-K and refrigerated moist chamber-stored corneas . Invest Ophthalmol 13:859-863, 1974. 6. Lindstrom RL, Doughman DJ, Van Horn DL, et al: Organ culture corneal storage at ambient room temperature . Arch Ophthalmol 95:869-878, 1977.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Loading next page...
 
/lp/american-medical-association/corneal-storage-at-room-temperature-Mc96PGTzL4
Publisher
American Medical Association
Copyright
Copyright © 1978 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1978.03910050595022
Publisher site
See Article on Publisher Site

Abstract

Abstract • Short-term eye banking is based mainly on moist chamber and McCarey-Kaufman medium (M-K medium) preservation. BOth involve a controlled 4 C temperature for storage. Warming the cornea to room temperature, however, drastically affects the endothelial viability. On enzymatic staining and histological study, the M-K medium-stored rabbit corneas had more normal endothelium than did "moist chamber" eyes when storage was prolonged for seven days at room temperature. In human corneas that were kept at 4 C for 24 hours and then exposed to a temperature of 25 C, destruction of organelles had occurred by six hours and was increased by 12 hours. Corneas that were kept in M-K medium had relatively intact endothelium after four days, but cell disruption and vacuolation was present by the seventh day. The M-K medium, therefore, affords protection to tissue warmed to room temperature, where metabolic activity is resumed. References 1. McCarey BE, Kaufman HE: Improved corneal storage . Invest Ophthalmol 13:165-173, 1974. 2. Bigar F, Kaufman HE, McCarey B, et al: Improved corneal storage for penetrating keratoplasties in man . Am J Ophthalmol 79:115-120, 1975. 3. Robbins JE, Capella JA, Kaufman HE: A study of endothelium in keratoplasty and corneal preservation . Arch Ophthalmol 73:242-247, 1965.Crossref 4. Polack FM: Scanning electron microscopy of corneal graft rejection . Invest Ophthalmol 11:1-14, 1972. 5. McCarey BE, Sakimoto T, Bigar F: Ultrastructure of M-K and refrigerated moist chamber-stored corneas . Invest Ophthalmol 13:859-863, 1974. 6. Lindstrom RL, Doughman DJ, Van Horn DL, et al: Organ culture corneal storage at ambient room temperature . Arch Ophthalmol 95:869-878, 1977.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Jun 1, 1978

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$499/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month