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Comparison of MAST With Radioallergosorbent and Skin Tests for Diagnosis of Allergy in Children

Comparison of MAST With Radioallergosorbent and Skin Tests for Diagnosis of Allergy in Children Abstract • The multiple allergosorbent test system (MAST) is a method for measuring total and allergen-specific IgE levels, using autoradiography and densitometry. The purpose of this study was to compare the results of MAST tests with those of radioallergosorbent (RAST) and skin tests as an adjunct to the diagnosis of allergy in children. Twenty children, aged 4 to 19 years, were studied. Total serum IgE level measured by the paper radioimmunosorbent test (PRIST) method ranged from 7 to 1,333 units/mL (geometric mean, 155 units/mL). Total serum IgE level by MAST significantly correlated with the PRIST IgE level. Quantities of allergen-specific IgE measured by MAST and RAST were also significantly correlated. When the diagnostic levels by MAST and RAST were compared with skin test reactions for ragweed, grass, house dust, and mite, MAST had a sensitivity of 59%, a specificity of 97%, and an efficiency of 72%, compared with 67%, 97%, and 78%, respectively, for the RAST analysis. We conclude that MAST and RAST are similar in their ability to measure allergen-specific IgE level, but that neither method is as sensitive as skin tests for detection of allergen-specific IgE. (AJDC 1986;140:45-48) References 1. Brostoff J, Pack S, Merritt T: A new multiple specific IgE assay—MAST . Lancet 1984;2: 748-749.Crossref 2. Booth BH III: Diagnosis of immediate hypersensitivity , in Patterson R (ed): Allergic Diseases: Diagnosis and Management , ed 2. Philadelphia, JB Lippincott Co, 1980, pp 87-88. 3. Miller SP, Marinkovich VA, Rege DH, et al: Application of the MAST immunodiagnostic system to the determination of allergen-specific IgE . Clin Chem 1984;30:1467-1472. 4. Ownby DR, Anderson JA, Jacobs GL, et al: Development and comparative evaluation of a multiple-antigen RAST as a screening test for inhalant allergy . J Allergy Clin Immunol 1984; 73:466-472.Crossref 5. Homburger HA, Jacobs GL: Analytic accuracy of specific immunoglobulin E antibody results determined by a blind proficiency survey . J Allergy Clin Immunol 1982;70:474-480.Crossref 6. Fox DJ, Guire KE: Documentation for MIDAS , ed 3. Ann Arbor, Mich, University of Michigan Press, 1978. 7. Galen RS, Gambino SR: Beyond Normality: The Predictive Value and Efficiency of Medical Diagnosis . New York, John Wiley & Sons Inc, 1975, pp 30-33. 8. American Academy of Allergy: Position statement: Skin testing and radioallergosorbent testing (RAST) for diagnosis of specific allergens responsible for IgE-mediated diseases . J Allergy Clin Immunol 1983;72:515-517.Crossref 9. Adkinson NF Jr: The radioallergosorbent test in 1981: Limitations and refinements . J Allergy Clin Immunol 1981;67:87-89.Crossref 10. Santrach PJ, Parker JL, Jones RT, et al: Diagnostic and therapeutic applications of a modified radioallergosorbent test and comparison with the conventional radioallergosorbent test . J Allergy Clin Immunol 1981;69:97-104.Crossref 11. Yunginger JW, Gleich GJ: The impact of the discovery of IgE on the practice of allergy . Pediatr Clin North Am 1975;22:3-15. 12. Norman PS, Lichtenstein LM, Ishizaka K: Diagnostic tests in ragweed hay fever: A comparison of direct skin tests, IgE and antibody measurements, and basophile histamine release . J Allergy Clin Immunol 1973;52:210-224.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Comparison of MAST With Radioallergosorbent and Skin Tests for Diagnosis of Allergy in Children

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Publisher
American Medical Association
Copyright
Copyright © 1986 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1986.02140150047031
Publisher site
See Article on Publisher Site

Abstract

Abstract • The multiple allergosorbent test system (MAST) is a method for measuring total and allergen-specific IgE levels, using autoradiography and densitometry. The purpose of this study was to compare the results of MAST tests with those of radioallergosorbent (RAST) and skin tests as an adjunct to the diagnosis of allergy in children. Twenty children, aged 4 to 19 years, were studied. Total serum IgE level measured by the paper radioimmunosorbent test (PRIST) method ranged from 7 to 1,333 units/mL (geometric mean, 155 units/mL). Total serum IgE level by MAST significantly correlated with the PRIST IgE level. Quantities of allergen-specific IgE measured by MAST and RAST were also significantly correlated. When the diagnostic levels by MAST and RAST were compared with skin test reactions for ragweed, grass, house dust, and mite, MAST had a sensitivity of 59%, a specificity of 97%, and an efficiency of 72%, compared with 67%, 97%, and 78%, respectively, for the RAST analysis. We conclude that MAST and RAST are similar in their ability to measure allergen-specific IgE level, but that neither method is as sensitive as skin tests for detection of allergen-specific IgE. (AJDC 1986;140:45-48) References 1. Brostoff J, Pack S, Merritt T: A new multiple specific IgE assay—MAST . Lancet 1984;2: 748-749.Crossref 2. Booth BH III: Diagnosis of immediate hypersensitivity , in Patterson R (ed): Allergic Diseases: Diagnosis and Management , ed 2. Philadelphia, JB Lippincott Co, 1980, pp 87-88. 3. Miller SP, Marinkovich VA, Rege DH, et al: Application of the MAST immunodiagnostic system to the determination of allergen-specific IgE . Clin Chem 1984;30:1467-1472. 4. Ownby DR, Anderson JA, Jacobs GL, et al: Development and comparative evaluation of a multiple-antigen RAST as a screening test for inhalant allergy . J Allergy Clin Immunol 1984; 73:466-472.Crossref 5. Homburger HA, Jacobs GL: Analytic accuracy of specific immunoglobulin E antibody results determined by a blind proficiency survey . J Allergy Clin Immunol 1982;70:474-480.Crossref 6. Fox DJ, Guire KE: Documentation for MIDAS , ed 3. Ann Arbor, Mich, University of Michigan Press, 1978. 7. Galen RS, Gambino SR: Beyond Normality: The Predictive Value and Efficiency of Medical Diagnosis . New York, John Wiley & Sons Inc, 1975, pp 30-33. 8. American Academy of Allergy: Position statement: Skin testing and radioallergosorbent testing (RAST) for diagnosis of specific allergens responsible for IgE-mediated diseases . J Allergy Clin Immunol 1983;72:515-517.Crossref 9. Adkinson NF Jr: The radioallergosorbent test in 1981: Limitations and refinements . J Allergy Clin Immunol 1981;67:87-89.Crossref 10. Santrach PJ, Parker JL, Jones RT, et al: Diagnostic and therapeutic applications of a modified radioallergosorbent test and comparison with the conventional radioallergosorbent test . J Allergy Clin Immunol 1981;69:97-104.Crossref 11. Yunginger JW, Gleich GJ: The impact of the discovery of IgE on the practice of allergy . Pediatr Clin North Am 1975;22:3-15. 12. Norman PS, Lichtenstein LM, Ishizaka K: Diagnostic tests in ragweed hay fever: A comparison of direct skin tests, IgE and antibody measurements, and basophile histamine release . J Allergy Clin Immunol 1973;52:210-224.Crossref

Journal

American Journal of Diseases of ChildrenAmerican Medical Association

Published: Jan 1, 1986

References