Abstract The fluorescent antinuclear (FA) test for the lupus erythematosus (LE) factor tends to vary in its sensitivity with several steps involved in its performance. With leukocytes its sensitiity may be quite close to that of the LE clot procedure, although with nuclear material such as neonatal thyroid or tumor cells, its sensitivity is high. However, with the increased sensitivity there appears to be decreased specificity with this procedure. In spite of these limitations the fluorescent antinuclear factor test is a valuable aid in the diagnosis of lupus erythematosus and related auto-immune diseases. In brief, the fluorescent antinuclear test is the more sensitive and the LE cell tests the more specific in the detection of the LE factor. References 1. Coons, A.H., and Kaplan, M.H.: Localization of Antigen in Tissue Cells: Improvements in a Method for the Detection of Antigen by Means of Fluorescent Antibody , J Exp Med 91:1-13 ( (Jan) ) 1950.Crossref 2. Vasquez, J.J., and Dixon, F.J.: Immunohistochemical Analysis of Amyloid by the Fluorescence Technique , J Exp Med 104:727-736 ( (Nov) ) 1956.Crossref 3. Weir, D.M.; Holborow, E.J.; and Johnson, G.D.: A Clinical Study of Serum Antinuclear Factor , Brit Med J 1:933-937 ( (April 1) ) 1961.Crossref 4. Widelock, D., et al: Fluorescent Antibody Procedure for Lupus Erythematosus: Comparative Use of Nucleated Erythrocytes and Calf Thymus Cells , Amer J Pub Health 51:829-835 ( (June) ) 1961. 5. Burnham, T.K.; Fine, G.; and Neblett, T.R.: Tumor Imprints as a Source of Nuclear Substrate for the Detection of Antinuclear Factors , J Invest Derm 43:7, 1964.Crossref 6. Haserick, J.R.; Lewis, L.A.; and Bortz, D.W.: Blood Factor of Acute Disseminated Lupus Erythematosus; Determination of Gamma Globulin as Specific Plasma Fraction , Amer J Med Sci 219:660-663 ( (June) ) 1950.Crossref 7. Haserick, J.R.: " Blood Factor in Lupus Erythematosus ," in MacKenna, R. M. B.: Modern Trends in Dermatology , second series, London: Butterworth & Co., Ltd., 1956, chap 5. 8. Goodman, H.C.; Fahey, J.L.; and Malmgren, R.A.: Serum Factors in Lupus Erythematosus and Other Diseases Reacting With Cell Nuclei and Nucleoprotein Extracts: Electrophoretic, Ultracentrifugal and Chromatographic Studies , J Clin Invest 39:1595-1605 ( (Oct) ) 1960.Crossref 9. Mandema, E., et al: Quantitative Observations on Antinuclear Factors in Systemic Lupus Erythematosus , J Lab Clin Med 58:337-352 ( (Sept) ) 1961. 10. Barnett, E.V., et al: Antinuclear Factors in Systemic Lupus Erythematosus and Rheumatoid Arthritis , Ann Intern Med 63:100, 1965.Crossref 11. Burnham, T.K.; Neblett, T.R.; and Fine, G.: The Immunofluorescent Tumor Imprint Technique: I. Description and Evaluation , Amer J Clin Path 45:714 ( (June) ) 1966. 12. Burnham, T.K.; Fine, G.; and Neblett, T.R.: The Immunofluorescent Tumor Imprint Technique: II. The Frequency of Antinuclear Factors in Connective Tissue Diseases and Dermatoses , Ann Intern Med 65:9 ( (July) ) 1966.Crossref 13. Hasker, J.; Mackay, I.R.; and Miller, J.J.: The Incidence of "Antinuclear Factor" in Human Disease , Aust Ann Med 14:96, 1965.
Archives of Dermatology – American Medical Association
Published: Nov 1, 1966