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CELLOIDIN AS AN EMBEDDING MEDIUM

CELLOIDIN AS AN EMBEDDING MEDIUM This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables. Abstract To the Editor: —The wholesale condemnation of celloidin as an embedding medium for ocular tissues by Wexler and Richardson, in their article entitled "Paraffin Method of Embedding Ocular Specimens for Microscopic Study," published in the March, 1955, issue of the Archives, page 365, will surprise workers using the modern rapid methods of embedding in nitrocellulose. The statement that celloidin causes more shrinkage than paraffin is contrary to the experience of workers in delicate zoological and botanical tissues. Shrinkage of the choroid in celloidin blocks is due to poor choice of fixative, formalin being one of the worst offenders. For routine work six hours' fixation in fresh Zenker's solution is ample. Unfortunately, the belief dies hard that it takes many days or weeks to fix an eye. Small pieces of iris need only half an hour. There is little shrinkage, and 20 minutes http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives of Ophthalmology American Medical Association

CELLOIDIN AS AN EMBEDDING MEDIUM

A.M.A. Archives of Ophthalmology , Volume 54 (5) – Nov 1, 1955

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Publisher
American Medical Association
Copyright
Copyright © 1955 American Medical Association. All Rights Reserved.
ISSN
0096-6339
DOI
10.1001/archopht.1955.00930020795024
Publisher site
See Article on Publisher Site

Abstract

This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables. Abstract To the Editor: —The wholesale condemnation of celloidin as an embedding medium for ocular tissues by Wexler and Richardson, in their article entitled "Paraffin Method of Embedding Ocular Specimens for Microscopic Study," published in the March, 1955, issue of the Archives, page 365, will surprise workers using the modern rapid methods of embedding in nitrocellulose. The statement that celloidin causes more shrinkage than paraffin is contrary to the experience of workers in delicate zoological and botanical tissues. Shrinkage of the choroid in celloidin blocks is due to poor choice of fixative, formalin being one of the worst offenders. For routine work six hours' fixation in fresh Zenker's solution is ample. Unfortunately, the belief dies hard that it takes many days or weeks to fix an eye. Small pieces of iris need only half an hour. There is little shrinkage, and 20 minutes

Journal

A.M.A. Archives of OphthalmologyAmerican Medical Association

Published: Nov 1, 1955

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