Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Cell Renewal in Human Epidermis

Cell Renewal in Human Epidermis Abstract Cell renewal in human epidermis was estimated after intradermal injection of thymidine-H3 in 21 subjects by means of serial biopsies and radioautographs. Results indicated 5% of germinative cells take up thymidine-H3 in 40 minutes. By seven days the number of labeled nuclei more than doubled and most were in the prickle layer. The first cell appeared in the granular layer in 10 to 14 days. After that a steady decline in number of labeled nuclei was observed. Epidermal cell renewal generally required 13 to 18 days. Epidermal cell turnover for man is similar to that reported for mouse and rat. The most important results challenge the older concept that epidermal cells divide and move in an orderly sequence. It now seems that mitosis and migration are random events. References 1. T indicates total turnover time of viable epidermal cells; ie, basal, prickle, and granular cells. BT indicates turnover time of germinative layer. BN/N indicates ratio of total number of germinative cells to total number of viable epidermal cells. 2. BT indicates turnover time of the germinative cell layer. TS indicates DNA synthesizing time. NS/BN indicates fraction of labeled cells to total cells in the germinative layer. 3. Sutton, R.L.: Early Epidermal Neoplasia , Arch Derm Syph 37:738-780, 1948. 4. Hoffman, J.G.: Quantitative Aspects of the Growth of Epidermis , Arch Path 47:37-43, 1949. 5. Pinkus, H.: Personal Communication. (Also cited by Rothberg et al, #4.) 6. Rothberg, S.; Crounse, R.G.; and Lee, J.L.: GlycineC14 Incorporation Into the Proteins of Normal Stratum Corneum and the Abnormal Stratum Corneum of Psoriasis , J Invest Derm 37:497-505, 1961.Crossref 7. Messier, B., and Leblond, C.P.: Preparation of Coated Radioautographs by Dipping Sections in Fluid Emulsion , Proc Soc Exp Biol Med 96:7-10, 1957.Crossref 8. Kopriwa, B.M., and Lebond, C.P.: Improvements in the Coating Technique of Radioautography , J Histochem Cytochem 10:269-284, 1962.Crossref 9. Leblond, C.P.; Greulich, R.C.; and Pereira, J.P.M.: " Relationship of Cell Formation and Cell Migration in the Renewal of Stratified Squamous Epithelia ," in Montagna, W., and Billingham, R.E. (eds.): Advances in Biology of Skin , New York: Pergamon Press, 1964, vol 5, pp 39-67. 10. Ferguson, E.H., and Epstein, W.L.: Clearance of I131 Injected Intralesionally in Patients with Psoriasis , J Invest Derm 37:441-444, 1961.Crossref 11. Downes, A.M.; Sharry, L.F.; and Till, A.R.: The Fate of Intradermal Doses of Labeled Amino Acids in Sheep , Aust J Biol Sci 17:945-959, 1964. 12. Greulich, R.C.: Aspects of Cell Individuality in the Renewal of Stratified Squamous Epithelia , in The Epidermis , Montagna, W., and Lobitz, W.C. (ed.), New York: Academic Press, 1964, pp 117-133. 13. Pilgrim, C., and Maurer, W.: Autoradiographische Bestimmung der DNS-verdopplungszeit verschiedener Zellarten von Maus und Ratte bei doppelmarkierung mit 3H- und 14C-thymidin , Naturwissenschaften 49:544-545, 1962.Crossref 14. Quastler, H., et al: Effect of X-Radiation on DNA Synthesis in Some Squamous Cell Epithelia , Radiol Res 16:561-562, 1962. 15. Defendi, V., and Manson, L.A.: Analysis of the Life-Cycle in Mammalian Cells , Nature 198:359, 1963.Crossref 16. Cameron, I.L., and Greulich, R.C.: Evidence for an Essentially Constant Duration of DNA-Synthesis in Renewing Epithelia of the Adult Mouse , J Cell Biol 18:31-40, 1963.Crossref 17. Cameron, I.L.: Is the Duration of DNA Synthesis in Somatic Cells of Mammals and Birds a Constant? , J Cell Biol 20:185-188, 1964. 18. Quastler, H., and Sherman, F.G.: Cell Population Kinetics in the Intestinal Epithelium of the Mouse , Exp Cell Res 17:420-438, 1959.Crossref 19. Greulich, R.C.: Personal communication to the author, June 1964. 20. Johnson, H.A., et al: A Radioautographic Study of a Human Brain and Glioblastoma Multiforme After the in Vitro Uptake of Tritiated Thymidine , Cancer 13:636-642, 1960.Crossref 21. Kaku, H.; Igarashi, Y.; and Fujita, S.: Cytokinetic Analysis of the Human Skin in Vivo in Normal and Pathologic Conditions: A 3H-Thymidine Autoradiographic Study , Arch Histol Jap 24:457-470, 1964.Crossref 22. Weinstein, G.D., and Van Scott, E.J.: Turnover Time in Normal and Psoriatic Human Epidermis by Autoradiographic Studies , abstracted from the program of the 26th Annual Meeting of the Society for Investigative Dermatology, June 20, 1965 . 23. Epstein, W.L., and Maibach, H.I.: Cell Renewal in Human Epidermis , abstracted, Clin Res 12:226, 1965. 24. Bertalanffy, F.D.: Tritiated Thymidine Versus Colchicine Technique in the Study of Cell Population Cytodynamics , Lab Invest 13:871-886, 1964. 25. Bertalanffy, F.D., and Lau, C.: Cell Renewal , Int Rev Cytol 13:357, 1962. 26. Iversen, O.H., and Bjerknes, R.: Kinetics of Epidermal Reaction to Carcinogens , Acta Path Microbiol Scand , (suppl 165) , 1963. 27. Skjaeggestad, O.: Experimental Epidermal Hyperplasia in Mice, Relation to Carcinogenesis , Acta Path Microbiol Scand , (suppl 169) , 1964. 28. Pinkus, H.: Keratosis Senilis , Amer J Path 29:193-207, 1958. 29. Mercer, E.H.: The Cancer Cell , Brit Med Bull 18:187-192, 1962. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Loading next page...
 
/lp/american-medical-association/cell-renewal-in-human-epidermis-yN5fWdy6WC
Publisher
American Medical Association
Copyright
Copyright © 1965 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1965.01600160118025
Publisher site
See Article on Publisher Site

Abstract

Abstract Cell renewal in human epidermis was estimated after intradermal injection of thymidine-H3 in 21 subjects by means of serial biopsies and radioautographs. Results indicated 5% of germinative cells take up thymidine-H3 in 40 minutes. By seven days the number of labeled nuclei more than doubled and most were in the prickle layer. The first cell appeared in the granular layer in 10 to 14 days. After that a steady decline in number of labeled nuclei was observed. Epidermal cell renewal generally required 13 to 18 days. Epidermal cell turnover for man is similar to that reported for mouse and rat. The most important results challenge the older concept that epidermal cells divide and move in an orderly sequence. It now seems that mitosis and migration are random events. References 1. T indicates total turnover time of viable epidermal cells; ie, basal, prickle, and granular cells. BT indicates turnover time of germinative layer. BN/N indicates ratio of total number of germinative cells to total number of viable epidermal cells. 2. BT indicates turnover time of the germinative cell layer. TS indicates DNA synthesizing time. NS/BN indicates fraction of labeled cells to total cells in the germinative layer. 3. Sutton, R.L.: Early Epidermal Neoplasia , Arch Derm Syph 37:738-780, 1948. 4. Hoffman, J.G.: Quantitative Aspects of the Growth of Epidermis , Arch Path 47:37-43, 1949. 5. Pinkus, H.: Personal Communication. (Also cited by Rothberg et al, #4.) 6. Rothberg, S.; Crounse, R.G.; and Lee, J.L.: GlycineC14 Incorporation Into the Proteins of Normal Stratum Corneum and the Abnormal Stratum Corneum of Psoriasis , J Invest Derm 37:497-505, 1961.Crossref 7. Messier, B., and Leblond, C.P.: Preparation of Coated Radioautographs by Dipping Sections in Fluid Emulsion , Proc Soc Exp Biol Med 96:7-10, 1957.Crossref 8. Kopriwa, B.M., and Lebond, C.P.: Improvements in the Coating Technique of Radioautography , J Histochem Cytochem 10:269-284, 1962.Crossref 9. Leblond, C.P.; Greulich, R.C.; and Pereira, J.P.M.: " Relationship of Cell Formation and Cell Migration in the Renewal of Stratified Squamous Epithelia ," in Montagna, W., and Billingham, R.E. (eds.): Advances in Biology of Skin , New York: Pergamon Press, 1964, vol 5, pp 39-67. 10. Ferguson, E.H., and Epstein, W.L.: Clearance of I131 Injected Intralesionally in Patients with Psoriasis , J Invest Derm 37:441-444, 1961.Crossref 11. Downes, A.M.; Sharry, L.F.; and Till, A.R.: The Fate of Intradermal Doses of Labeled Amino Acids in Sheep , Aust J Biol Sci 17:945-959, 1964. 12. Greulich, R.C.: Aspects of Cell Individuality in the Renewal of Stratified Squamous Epithelia , in The Epidermis , Montagna, W., and Lobitz, W.C. (ed.), New York: Academic Press, 1964, pp 117-133. 13. Pilgrim, C., and Maurer, W.: Autoradiographische Bestimmung der DNS-verdopplungszeit verschiedener Zellarten von Maus und Ratte bei doppelmarkierung mit 3H- und 14C-thymidin , Naturwissenschaften 49:544-545, 1962.Crossref 14. Quastler, H., et al: Effect of X-Radiation on DNA Synthesis in Some Squamous Cell Epithelia , Radiol Res 16:561-562, 1962. 15. Defendi, V., and Manson, L.A.: Analysis of the Life-Cycle in Mammalian Cells , Nature 198:359, 1963.Crossref 16. Cameron, I.L., and Greulich, R.C.: Evidence for an Essentially Constant Duration of DNA-Synthesis in Renewing Epithelia of the Adult Mouse , J Cell Biol 18:31-40, 1963.Crossref 17. Cameron, I.L.: Is the Duration of DNA Synthesis in Somatic Cells of Mammals and Birds a Constant? , J Cell Biol 20:185-188, 1964. 18. Quastler, H., and Sherman, F.G.: Cell Population Kinetics in the Intestinal Epithelium of the Mouse , Exp Cell Res 17:420-438, 1959.Crossref 19. Greulich, R.C.: Personal communication to the author, June 1964. 20. Johnson, H.A., et al: A Radioautographic Study of a Human Brain and Glioblastoma Multiforme After the in Vitro Uptake of Tritiated Thymidine , Cancer 13:636-642, 1960.Crossref 21. Kaku, H.; Igarashi, Y.; and Fujita, S.: Cytokinetic Analysis of the Human Skin in Vivo in Normal and Pathologic Conditions: A 3H-Thymidine Autoradiographic Study , Arch Histol Jap 24:457-470, 1964.Crossref 22. Weinstein, G.D., and Van Scott, E.J.: Turnover Time in Normal and Psoriatic Human Epidermis by Autoradiographic Studies , abstracted from the program of the 26th Annual Meeting of the Society for Investigative Dermatology, June 20, 1965 . 23. Epstein, W.L., and Maibach, H.I.: Cell Renewal in Human Epidermis , abstracted, Clin Res 12:226, 1965. 24. Bertalanffy, F.D.: Tritiated Thymidine Versus Colchicine Technique in the Study of Cell Population Cytodynamics , Lab Invest 13:871-886, 1964. 25. Bertalanffy, F.D., and Lau, C.: Cell Renewal , Int Rev Cytol 13:357, 1962. 26. Iversen, O.H., and Bjerknes, R.: Kinetics of Epidermal Reaction to Carcinogens , Acta Path Microbiol Scand , (suppl 165) , 1963. 27. Skjaeggestad, O.: Experimental Epidermal Hyperplasia in Mice, Relation to Carcinogenesis , Acta Path Microbiol Scand , (suppl 169) , 1964. 28. Pinkus, H.: Keratosis Senilis , Amer J Path 29:193-207, 1958. 29. Mercer, E.H.: The Cancer Cell , Brit Med Bull 18:187-192, 1962.

Journal

Archives of DermatologyAmerican Medical Association

Published: Oct 1, 1965

References