Abstract Since the normal biochemistry of the ocular tissues is practically unknown, it is difficult to determine the course of events leading to pathologic changes. One method of studying the biochemistry of a tissue is to analyze the tissues and to determine quantitatively the amounts of the various major constituents. In a previous series of articles,1 too numerous to mention here, the results of the analyses of two major constituents of the ocular tissues, i. e., the proteins and the lipids, were reported. During the past two years interest was turned toward an inquiry into the nature of the third constituent, the water-soluble extractives. In most of the ocular tissues these substances occur in minute quantities and are difficult to isolate and identify chemically. Frequently the water-soluble extractives can be determined only quantitatively, one at a time, in the tissues. Consequently the investigation may be limited to a few References 1. Krause, A. C.: The Biochemistry of the Eye , Baltimore, Johns Hopkins Press, 1934. 2. Eggleston, M. G., and Eggleston, P.: Carnosine in Amphibian Voluntary Muscle , Quart. J. Exper. Physiol. 23:391, 1933. 3. Koessler, K. K., and Hanke, M. T.: A Microchemical Colorimetric Method for Estimating Imidazole Derivatives , J. Biol. Chem. 39:497, 1919. 4. Wilson, D. W., and Wolff, W. A.: The Determination of Anserine in Muscle , .J. Biol. Chem. 100:cvi, 1933. 5. Folin, O., and Marenzi, A. D.: Tyrosine and Triptophane Determinations in One-Tenth Gram of Protein , J. Biol. Chem. 83:89, 1929. 6. Gulewitsch, W., and Amiradžibi, S.: Zur Kenntnis der Extractivstoffe der Muskeln , Ztschr. f. physiol. Chem. 30:565, 1900.Crossref 7. Ackermann, D.; Timpe, O., and Poller, K.: Ueber das Anserin, einen neuen Bestandteil der Vogelmuskulatur , Ztschr. f. physiol. Chem. 183:1, 1929.Crossref 8. Wolff, W. A., and Wilson, D. W.: Anserine in Mammalian Skeletal Muscle , J. Biol. Chem. 95:495, 1932 9. Carnosine and Anserine in Mammalian Skeletal Muscle , Wolff Anserine in Mammalian Skeletal Muscle 109:565, 1935. 10. Rasenkow, I. P.; Derweis, G. W., and Ssewerin, S. E.: Zur Frage nach Carnosinwirkung auf die Magensaftsekretion , Ztschr. f. physiol. Chem. 162:95, 1926.Crossref 11. Blanchetière, M. A.: Constitution des anhydrides des acides aspartique et glutamique; son importance biologique , Bull. Soc. chim. biol. 6:854, 1924.
Archives of Ophthalmology – American Medical Association
Published: Dec 1, 1936
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