Abstract Inflammatory changes were produced in the human skin by means of: (1) physical trauma (cellophane tape stripping); (2) application of a primary irritant (phenol); (3) sensitization (2,4,dinitrochlorobenzene). An ointment containing nicotinic acid was used as a vasodilator substance. Changes of the minute cutaneous vessels were observed and photographed. A modified technique of capillary microscopy, using vertical illumination and a dipping cone, is presented. A pattern of vascular reaction emerged which followed its course regardless of the type of injury used. The degree to which this pattern was followed seems to depend upon the amount of damage produced. The pattern of reaction could be divided into four phases: ( 1) opening of closed vessels; (2) vascular damage; (3) vascular proliferation; (4) vascular recovery. There is not always a correlation between the gross appearance of the stimulated area and the microscopic anatomic vascular change, as demonstrable by the present technique. References 1. Lewis, T.: The Blood Vessels of the Human Skin and Their Responses , London: Shaw and Sons, 1927. 2. Krogh, A.: The Anatomy and Physiology of Capillaries , New Haven, Conn: Yale University Press, 1929. 3. Bordley, J., III; Gross, M. H.; and Sherman, W. B.: Intermittent Flow in Capillaries of Human Skin , Bull Hopkins Hosp 62:1, 1938. 4. Wolf, J.: Die Innere Struktur der Zellen des Stratum desquamativum der menschlichen Epidermis , Z Mikr Anat Forsch 46:170, 1939. 5. Pinkus, H.: Examination of the Epidermis by the Strip Method of Removing Horny Layers , J Invest Derm 16:383, 1951. 6. Gilje, O.: Capillary Microscopy in the Differential Diagnosis of Skin Diseases , Acta Dermatovener 33:303, 1953. 7. Gilje, O.; O'Leary, P. A.; and Baldes, E. J.: Capillary Microscopic Examination in Skin Diseases , AMA Arch Derm Syph 68:136, 1953.Crossref 8. Davis, M. J., and Lorincz, A. L.: An Improved Technique for Capillary Microscopy of the Skin , J Invest Derm 28:283, 1957. 9. Goldman, L.: Clinical Studies in Microscopy of the Skin at Moderate Magnification, Summary of 10 Years Experience , AMA Arch Derm Syph 75:345, 1957.Crossref 10. Wells, G. C.: The Effect of Hydrocortisone on Standardized Skin-Surface Trauma , Brit J Derm 69:11, 1957.Crossref 11. Davis, M. J., and Lawler, J. C.: The Capillary Circulation of the Skin , AMA Arch Derm Syph 77:690, 1958.Crossref 12. Davis, M. J., and Lawler, J. C.: Capillary Alterations in Pigmented Purpuric Disease of the Skin , AMA Arch Derm Syph 78:723, 1958.Crossref 13. Fulton, G. P.; Farber, E. M.; and Moreci, A. P.: The Mechanism of Action of Rubefacients , J Invest Derm 33:317, 1959.Crossref 14. Moller, H., and Rorsman, H.: Vascular Effects of Epidermal Stripping , Acta Dermatovener 40:381, 1960. 15. Davis, M. J.; Demis, D. J.; and Lawler, J. C.: The Microcirculation of the Skin , J Invest Derm 34:31, 1960. 16. Lawler, J. C., and Vineyard, H. R.: The Effect of Treatment on the Vascular Component of the Psoriatic Lesion , Arch Derm Syph 82:183, 1960.Crossref 17. Telner, P., and Fekete, Z.: The Capillary Responses in Psoriatic Skin , J Invest Derm 36:225, 1961. 18. Lawler, J. C., and Lumpkin, L. R.: Cutaneous Capillary Changes in Lupus Erythematosus , Arch Derm Syph 83:636, 1961.Crossref 19. Miles, A. A., and Wilhelm, D. L.: Enzyme-Like Globulins From Serum Reproducing the Vascular Phenomena of Inflammation , Brit J Exp Path 36:71, 1955. 20. Ungar, G.: The Mechanism of Inflammation: An International Symposium , edited by G. Jasmin and A. Robert, Montreal, Canada: Acta, Inc., 1953, p 151. 21. Wilhelm, D. L., and Mason, B.: Vascular Permeability Changes in Inflammation: The Role of Endogenous Permeability Factors in Mild Thermal Injury , Brit J Exp Path 41:487, 1960. 22. Spector, W. G.: " The Mechanism of Inflammation ," in Progress in the Biological Sciences in Relation to Dermatology: A Symposium , edited by Arthur Rook, Cambridge University Press, 1960, p 295. 23. Rothman, S.: The Physiology and Biology of the Skin , University of Chicago Press, 1954, p 80. 24. Illig, L.: Pathologie und Klinik in Einzeldarstellungen, vol 10. Die terminale Strombahn: Capillarbett und Mikrozirkulation , Berlin: Springer-Verlag, 1961.
Archives of Dermatology – American Medical Association
Published: Sep 1, 1963