Abstract Increased tissue iron in patients with pernicious anemia was first described in 1910, by Ryffel,1 who used autopsy material. Increased hemosiderin in bone marrow aspirates was demonstrated in 1948, by Rath and Finch,2 who showed that such a rise in bone marrow hemosiderin is common to all anemias except that of iron deficiency and that it is due largely to a shift of iron from the red blood cells to storage.3 Beutler recently reviewed quantitative aspects of iron stores.4 The present study of marrow hemosiderin deals with its pattern of distribution rather than its total amount. Whenever possible observations on its microscopic appearance were correlated with data obtained from ferrokinetic measurements. Methods and Material Bone marrow aspirations were performed using a 16 gauge needle* and a 10 ml. syringe previously rinsed with a 10% solution of edathamil disodium (Disodium Versenate). The aspirate was ejected on an References 1. 31-16-01. 2. Performed by Dr. Nicholas Petrakis. 3. Ryffel, J. H.: The Amount of Iron in the Organs in Cases of Pernicious Anemia , J. Path. & Bact. 14:411-413, 1910. 4. Rath, C. E., and Finch, C. A.: Sternal Marrow Hemosiderin: Method for Determination of Available Iron Stores in Man , J. Lab. & Clin. Med. 33:81-86, 1948. 5. Finch, C. A., et al.: Iron Metabolism: Pathophysiology of Iron Storage , Blood 5:983-1008, 1950. 6. Beutler, E.: Clinical Evaluation of Iron Stores , New England J. Med. 256:692-697, 1957. 7. Pollycove, M.: Eisenstoffwechsel , in Thannhausers Lehrbuch des Stoffwechsels und der Stoffwechselkrankheiten , edited by N. Zöllner, Ed. 12, Stuttgart, Georg Thieme Verlag, 1957, pp. 837, 861. 8. Dacie, J. V., and Doniach, I.: Basophilic Property of Iron-Containing Granules in Siderocytes , J. Path. & Bact. 59:684-686, 1947. 9. McFadzean, A. J. S., and Davis, L. J.: Iron-Staining Erythrocyte Inclusions with Especial Reference to Acquired Haemolytic Anaemia , Glasgow M. J. 28:237-279, 1947. 10. Kaplan, E.; Zuelzer, W. W., and Mouriquand, C.: Sideroblasts: Study of Stainable Nonhemoglobin Iron in Marrow Normoblasts , Blood 9:203-213, 1954. 11. Douglas, A. S., and Dacie, J. V.: Incidence and Significance of Iron-Containing Granules in Human Erythrocytes and Their Precursors , J. Clin. Path. 6:307-313, 1953. 12. Apt, L.; Pollycove, M., and Ross, J. F.: Idiopathic Pulmonary Hemosiderosis: A Study of the Anemia and Iron Distribution Using Radioiron and Radiochromium , J. Clin. Invest. 36:1150-1159, 1957. 13. Pollycove, M., and Mortimer, R.: The Quantitative Determination of Individual Iron Kinetics and Hemoglobin Formation in Human Subjects, Abstract , Clin. Res. Proc. 4:51, 1956.
A.M.A. Archives of Internal Medicine – American Medical Association
Published: Feb 1, 1958