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Biochemical Studies of Subretinal Fluid: III. Ascorbic Acid of Subretinal Fluid in Patients With Retinal Detachments

Biochemical Studies of Subretinal Fluid: III. Ascorbic Acid of Subretinal Fluid in Patients With... Abstract The purpose of this paper is to report on the ascorbic acid content of subretinal fluid (SRF) in 75 cases of idiopathic retinal detachment, relating the findings to certain clinical data. Heath et al1 (27 cases) reported that the mean "total" ascorbic acid * content of SRF from eyes in which no retinal break could be found was significantly lower than in those having a visible retinal hole. In all strictly idiopathic cases, the ascorbic acid content of the SRF was found to be from 2-24 times that of the blood. De Vincentiis3 determined the "true" ascorbic acid † content of SRF in 12 cases and found it to be from 3 to 6.5 times the plasma level. In three cases, the SRF level of "true" ascorbic acid was from 50% to 75% of the aqueous level. Materials and Methods The subretinal fluid from 75 cases was collected, tested References 1. "Total" ascorbic acid, as determined by the dinitrophenylhydrazine method of Roe and Kuether,2 represents the sum of ascorbic, dehydroascorbic, and diketogulonic acids. 2. "True" ascorbic acid is determined by the dichlorophenol-indophenol method of Mindlin and Butler,4 which gives lower values than dinitrophenylhydrazine methods.5 3. Heath, H.; Beck, T. C.; and Foulds, W. S.: Chemical Composition of Subretinal Fluid , Brit J Ophthal 46:385-396, 1962.Crossref 4. Roe, J. H., and Kuether, C. A.: Determination of Ascorbic Acid in Whole Blood and Urine Through 2,4-Dinitrophenylhydrazine Derivative of Dehydroascorbic Acid , J Biol Chem 147:399-407, 1943. 5. de Vincentiis, M.: Further Observations on Chemical Composition of Subretinal Fluid , Arch Ottal B Air 62:5-17, 1958. 6. Mindlin, R. L., and Butler, A. M.: Determination of Ascorbic Acid in Plasma , J Biol Chem 122: 673-686, 1938. 7. Sabry, J. H., and Dodds, M. L.: Comparative Measurements of Ascorbic Acid and Total Ascorbic Acid of Blood Plasma , J Nutr 64:467-473, 1958. 8. Weber, J. C., and Wilson, F. M.: Biochemical Studies of Subretinal Fluid: II. Total Protein and Albumin of Subretinal Fluid and Blood Serum in Patients With Retinal Detachment , Arch Ophthal 69:363-369, 1963. 9. Bessey, O. A.: " Microchemical Methods ," in Vitamin Methods , P. Gyorgy, ed., New York: Academic Press, Inc., 1950, vol 1, pp 303-305. 10. Geschwind, I. I.; Williams, B. S.; and Li, C. H.: Simplification of Roe and Kuether Procedure for Ascorbic Acid Determinations in Adrenal Glands , Acta Endocr (Kobenhavn) 8:247-250, 1951. 11. Lowry, O. H.; Lopez, J. A.; and Bessey, O. A.: Determination of Ascorbic Acid in Small Amounts of Blood Serum , J Biol Chem 160:609-615, 1945. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Biochemical Studies of Subretinal Fluid: III. Ascorbic Acid of Subretinal Fluid in Patients With Retinal Detachments

Archives of Ophthalmology , Volume 71 (4) – Apr 1, 1964

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Publisher
American Medical Association
Copyright
Copyright © 1964 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1964.00970010572024
Publisher site
See Article on Publisher Site

Abstract

Abstract The purpose of this paper is to report on the ascorbic acid content of subretinal fluid (SRF) in 75 cases of idiopathic retinal detachment, relating the findings to certain clinical data. Heath et al1 (27 cases) reported that the mean "total" ascorbic acid * content of SRF from eyes in which no retinal break could be found was significantly lower than in those having a visible retinal hole. In all strictly idiopathic cases, the ascorbic acid content of the SRF was found to be from 2-24 times that of the blood. De Vincentiis3 determined the "true" ascorbic acid † content of SRF in 12 cases and found it to be from 3 to 6.5 times the plasma level. In three cases, the SRF level of "true" ascorbic acid was from 50% to 75% of the aqueous level. Materials and Methods The subretinal fluid from 75 cases was collected, tested References 1. "Total" ascorbic acid, as determined by the dinitrophenylhydrazine method of Roe and Kuether,2 represents the sum of ascorbic, dehydroascorbic, and diketogulonic acids. 2. "True" ascorbic acid is determined by the dichlorophenol-indophenol method of Mindlin and Butler,4 which gives lower values than dinitrophenylhydrazine methods.5 3. Heath, H.; Beck, T. C.; and Foulds, W. S.: Chemical Composition of Subretinal Fluid , Brit J Ophthal 46:385-396, 1962.Crossref 4. Roe, J. H., and Kuether, C. A.: Determination of Ascorbic Acid in Whole Blood and Urine Through 2,4-Dinitrophenylhydrazine Derivative of Dehydroascorbic Acid , J Biol Chem 147:399-407, 1943. 5. de Vincentiis, M.: Further Observations on Chemical Composition of Subretinal Fluid , Arch Ottal B Air 62:5-17, 1958. 6. Mindlin, R. L., and Butler, A. M.: Determination of Ascorbic Acid in Plasma , J Biol Chem 122: 673-686, 1938. 7. Sabry, J. H., and Dodds, M. L.: Comparative Measurements of Ascorbic Acid and Total Ascorbic Acid of Blood Plasma , J Nutr 64:467-473, 1958. 8. Weber, J. C., and Wilson, F. M.: Biochemical Studies of Subretinal Fluid: II. Total Protein and Albumin of Subretinal Fluid and Blood Serum in Patients With Retinal Detachment , Arch Ophthal 69:363-369, 1963. 9. Bessey, O. A.: " Microchemical Methods ," in Vitamin Methods , P. Gyorgy, ed., New York: Academic Press, Inc., 1950, vol 1, pp 303-305. 10. Geschwind, I. I.; Williams, B. S.; and Li, C. H.: Simplification of Roe and Kuether Procedure for Ascorbic Acid Determinations in Adrenal Glands , Acta Endocr (Kobenhavn) 8:247-250, 1951. 11. Lowry, O. H.; Lopez, J. A.; and Bessey, O. A.: Determination of Ascorbic Acid in Small Amounts of Blood Serum , J Biol Chem 160:609-615, 1945.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Apr 1, 1964

References