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Bacteriologic Safety of Using Tissue-Culture Storage

Bacteriologic Safety of Using Tissue-Culture Storage Abstract To the Editor.–Regarding the report in your November issue of the Archives from LeFrancois and Baum (94:1907-1909, 1976) concerning a case of Flavobacterium endophthalmitis following penetrating keratoplasty, we would like to make note of the following. Our concern as to the bacteriologic safety of using tissue-culture storage (McCarey-Kaufman media) led us to culture 25 consecutive McCarey-Kaufman media and corneal-scleral remnants within the media immediately following keratoplasties; all cultures proved to be negative.1 In view of this and the absence of any prior report of bacterial endophthalmitis since the introduction of McCarey-Kaufman media in 1974, we are not sure whether this one case report indicates a need for changes in preoperative technique, ie, delaying surgery for 48 hours pending culture reports, as suggested by LeFrancois and Baum. Furthermore, since no technique actually sterilizes eye tissue, leaving the tissue in the culture media for such increased amounts of time may References 1. Keates RH, Mishler KE: Corneal storage media. Ophthalmic Surgery, to be published. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Bacteriologic Safety of Using Tissue-Culture Storage

Mishler, Ken; Keates, Richard H.
Archives of Ophthalmology , Volume 95 (3) – Mar 1, 1977
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Bacteriologic Safety of Using Tissue-Culture Storage

Abstract

Abstract To the Editor.–Regarding the report in your November issue of the Archives from LeFrancois and Baum (94:1907-1909, 1976) concerning a case of Flavobacterium endophthalmitis following penetrating keratoplasty, we would like to make note of the following. Our concern as to the bacteriologic safety of using tissue-culture storage (McCarey-Kaufman media) led us to culture 25 consecutive McCarey-Kaufman media and corneal-scleral remnants within the media immediately following...
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Publisher
American Medical Association
Copyright
Copyright © 1977 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1977.04450030161024
Publisher site
See Article on Publisher Site

Abstract

Abstract To the Editor.–Regarding the report in your November issue of the Archives from LeFrancois and Baum (94:1907-1909, 1976) concerning a case of Flavobacterium endophthalmitis following penetrating keratoplasty, we would like to make note of the following. Our concern as to the bacteriologic safety of using tissue-culture storage (McCarey-Kaufman media) led us to culture 25 consecutive McCarey-Kaufman media and corneal-scleral remnants within the media immediately following keratoplasties; all cultures proved to be negative.1 In view of this and the absence of any prior report of bacterial endophthalmitis since the introduction of McCarey-Kaufman media in 1974, we are not sure whether this one case report indicates a need for changes in preoperative technique, ie, delaying surgery for 48 hours pending culture reports, as suggested by LeFrancois and Baum. Furthermore, since no technique actually sterilizes eye tissue, leaving the tissue in the culture media for such increased amounts of time may References 1. Keates RH, Mishler KE: Corneal storage media. Ophthalmic Surgery, to be published.

Journal

Archives of Ophthalmology – American Medical Association

Published: Mar 1, 1977

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