Abstract The exact site of the antigen-antibody reaction in allergic contact dermatitis is unknown. Early investigators were proponents of the humoral theory, believing that the site of the antigen-antibody reaction is in the blood stream. Supporters of the currently more popular cellular theory offer evidence to indicate that the site is in or on the tissue cells.16 In 1942, Coons and co-workers first demonstrated the value of using a fluorescent antibody to identify antigens in tissue.1 In the past decade there have been many refinements in the procedure,3 and the technique has been used successfully in the identification of micro-organisms5,6,10,11 and in the immunohistochemical investigation of connective tissue diseases and many other pathologic states.14,17 This paper reports the successful results of application of the technique to localize the site of the antigenantibody reaction in human subjects in experimentally produced Rhus dermatitis. Materials and Methods A. References 1. Coons, A. H.: Fluorescent Antibody Methods , in General Cytochemical Methods , edited by J. F. Daniell, New York, Academic Press, Inc., 1958, Vol. 1, pp. 399-422. 2. Coons, A. H.; Creech, H. J.; Jones, R. N., and Berliner, E.: Demonstration of Pneumococcal Antigen in Tissues by Use of Fluorescent Antibody , J. Immunol. 45:159-170, 1942. 3. Coons, A. H., and Kaplan, M. H.: Localization of Antigen in Tissue Cells , J. Exper. Med. 91:1-13, 1950.Crossref 4. Coons, A. H.; Leduc, E. H., and Kaplan, M. H.: Localization of Antigen in Tissue Cells , J. Exper. Med. 93:173-188, 1951.Crossref 5. Deacon, W. E.; Falcone, V. H., and Harris, Ad.: A Fluorescent Test for Treponemal Antibodies , Proc. Soc. Exper. Biol. & Med. 96:477-480, 1957. 6. Deacon, W. E.; Peacock, W. L., Jr.; Freeman, E. M., and Harris, A.: Identification of Neisseria Gonorrhoeae by Means of Fluorescent Antibodies , Proc. Soc. Exper. Biol. & Med. 101: 322-325, 1959. 7. Epstein, W. L., and Kligman, A. M.: Some Factors Affecting the Reaction of Allergic Contact Dermatitis , J. Invest. Dermat. 33:231-243, 1959. 8. Epstein, W. L., and Kligman, A. M.: Transfer of Allergic Contact-Type Delayed Sensitivity in Man , J. Invest. Dermat. 28:291-304, 1957. 9. Goldman, M.: Personal communication to the author, Communicable Disease Center, Atlanta, Aug., 1959. 10. Goldman, M.: Use of Fluorescein-Tagged Antibody to Identify Cultures of Endamoeba Histolytica and Endamoeba Coli , Am. J. Hyg. 59: 318-325, 1954. 11. Gordon, M. A.: Differentiation of Yeasts by Means of Fluorescent Antibody , Proc. Soc. Exper. Biol. & Med. 97:694-698, 1958. 12. Gornall, A. G.; Bardawill, G. J., and David, M. N.: Determination of Serum Proteins by Means of the Biuret Reaction , J. Biol. Chem. 177:751-766, 1959. 13. Kabat, E. A.: Immunochemistry in Progress in Allergy II , edited by Paul Kallos, New York, S. Karger A. G., 1949. 14. Kaplan, M. H.: The Fluorescent Antibody Technic as a Research Tool in the Study of Connective Tissue Disease , Arthritis Rheum. 2:568-573, 1959. 15. Kligman, A.: Poison Ivy (Rhus) Dermatitis , A.M.A. Arch. Dermat. 77:149-178, 1958. 16. Vaughan, W. T., and Black, J. H.: Practice of Allergy , St. Louis, C. V. Mosby Company, 1954, pp. 21-30. 17. Vazquez, J. J., and Dixon, F. J.: Immunohistochemical Study of Lesions in Rheumatic Fever, Systemic Lupus Erythematosus, and Rheumatoid Arthritis , Lab. Invest. 6:205-217, 1957. 18. Warwick, W. J.; Page, A., and Good, R. A.: Passive Transfer with Circulating Leukocytes of Delayed Hypersensitivity to Cat Scratch Antigen , Proc. Soc. Exper. Biol. & Med. 93:253-255, 1956.
Archives of Dermatology – American Medical Association
Published: Mar 1, 1961