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Antibody Formation in Rabbit Eye Studied with Huorescein-Labeled Antibody

Antibody Formation in Rabbit Eye Studied with Huorescein-Labeled Antibody Abstract Previous experimental work with Leptospira pomona infection in the rabbit eye indicates that antibody formation takes place locally and is related to the infiltration of the uveal tissue by plasma cells and lymphocytes. This concept is based on the observations that the antibody content of the gamma-globulin of the aqueous humor ("antibody activity") in local infection was higher than the activity of the serum and that the amount of local antibody activity was roughly correlated with the degree of plasma cell infiltration of the uveal tissue.1 The following study was undertaken to determine whether plasma cells are responsible for antibody production in the uveal tissue of the inflamed eye. Coons and coworkers2 have shown that antibodies within cells can be made visible by a variation of their method, using fluorescein-labeled antibody. Frozen sections of tissues containing antibody are dipped in a dilute solution of homologous antigen, then carefully References 1. References 4 and 5. 2. Witmer, R.: Experimental Leptospiral Uveitis in Rabbits , A. M. A. Arch. Ophth. 53:547-569, 1955.Crossref 3. Coons, A. H.; Leduc, E. H., and Connolly, J. M.: Immunohistochemical Studies of Antibody Response in the Rabbit , Fed. Proc. 12:1444, 1953. 4. Stavitsky, A. B.: Micromethods for the Study of Proteins and Antibodies , J. Immunol. 72:360-375, 1954. 5. Coons, A. H., and Kaplan, M. H.: Localization of Antigen in Tissue Cells: II. Improvement in a Method for the Detection of Antibodies by Means of Fluorescent Antibody , J. Exper. Med. 91:1-13, 1950.Crossref 6. Coons, A. H.; Leduc, E. H., and Kaplan, M. H.: Localization of Antigen in Tissue Cells: IV. Fate of Injected Foreign Protein in the Mouse , J. Exper. Med. 93:173-188, 1951.Crossref 7. Coffin, D. L.; Coons, A. H., and Cabasso, V. J.: A Histological Study of Infectious Hepatitis by Means of Fluorescent Antibody , J. Exper. Med. 98:13-20, 1953.Crossref 8. Seegal, B. C., and Seegal, D.: Local Organ Hypersensitiveness: VII. Demonstration of Agglutinins in Tissues of Rabbit Eye Following Immunization with Eb. Typhi Vaccine , Proc. Soc. Exper. Biol. & Med. 31:437-441, 1934. 9. Goldmann, H., and Witmer, R.: Antikörper im Kammerwasser , Ophthalmologica 127:323-330, 1954. 10. Ashton, N.: Allergic Factors in the Aetiology of Uveitis, to be published. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives of Ophthalmology American Medical Association

Antibody Formation in Rabbit Eye Studied with Huorescein-Labeled Antibody

A.M.A. Archives of Ophthalmology , Volume 53 (6) – Jun 1, 1955

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Publisher
American Medical Association
Copyright
Copyright © 1955 American Medical Association. All Rights Reserved.
ISSN
0096-6339
DOI
10.1001/archopht.1955.00930010819005
Publisher site
See Article on Publisher Site

Abstract

Abstract Previous experimental work with Leptospira pomona infection in the rabbit eye indicates that antibody formation takes place locally and is related to the infiltration of the uveal tissue by plasma cells and lymphocytes. This concept is based on the observations that the antibody content of the gamma-globulin of the aqueous humor ("antibody activity") in local infection was higher than the activity of the serum and that the amount of local antibody activity was roughly correlated with the degree of plasma cell infiltration of the uveal tissue.1 The following study was undertaken to determine whether plasma cells are responsible for antibody production in the uveal tissue of the inflamed eye. Coons and coworkers2 have shown that antibodies within cells can be made visible by a variation of their method, using fluorescein-labeled antibody. Frozen sections of tissues containing antibody are dipped in a dilute solution of homologous antigen, then carefully References 1. References 4 and 5. 2. Witmer, R.: Experimental Leptospiral Uveitis in Rabbits , A. M. A. Arch. Ophth. 53:547-569, 1955.Crossref 3. Coons, A. H.; Leduc, E. H., and Connolly, J. M.: Immunohistochemical Studies of Antibody Response in the Rabbit , Fed. Proc. 12:1444, 1953. 4. Stavitsky, A. B.: Micromethods for the Study of Proteins and Antibodies , J. Immunol. 72:360-375, 1954. 5. Coons, A. H., and Kaplan, M. H.: Localization of Antigen in Tissue Cells: II. Improvement in a Method for the Detection of Antibodies by Means of Fluorescent Antibody , J. Exper. Med. 91:1-13, 1950.Crossref 6. Coons, A. H.; Leduc, E. H., and Kaplan, M. H.: Localization of Antigen in Tissue Cells: IV. Fate of Injected Foreign Protein in the Mouse , J. Exper. Med. 93:173-188, 1951.Crossref 7. Coffin, D. L.; Coons, A. H., and Cabasso, V. J.: A Histological Study of Infectious Hepatitis by Means of Fluorescent Antibody , J. Exper. Med. 98:13-20, 1953.Crossref 8. Seegal, B. C., and Seegal, D.: Local Organ Hypersensitiveness: VII. Demonstration of Agglutinins in Tissues of Rabbit Eye Following Immunization with Eb. Typhi Vaccine , Proc. Soc. Exper. Biol. & Med. 31:437-441, 1934. 9. Goldmann, H., and Witmer, R.: Antikörper im Kammerwasser , Ophthalmologica 127:323-330, 1954. 10. Ashton, N.: Allergic Factors in the Aetiology of Uveitis, to be published.

Journal

A.M.A. Archives of OphthalmologyAmerican Medical Association

Published: Jun 1, 1955

References

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