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An Effect of Exercise, Skin Shock, and Ascorbic Acid on Serum Oxidase Activity

An Effect of Exercise, Skin Shock, and Ascorbic Acid on Serum Oxidase Activity Abstract In 1957 Akerfeldt1 reported that when the N,N-dimethyl form of p-phenylenediamine (PPD) is added to serum the PPD becomes oxidized to a red-colored substance. He found that serum from schizophrenic patients oxidized PPD at a faster rate than serum from normal controls. A rapid rate of oxidation was also found in a large percentage of patients with liver disease, neoplasm, and pregnancy in the last trimester.2 In this reaction a 0.1% solution of N,N-dimethyl-p-phenylenediamine is added to an equal quantity of serum. The increase in optical density is then measured spectrophotometrically for six minutes at wavelength 552 mμ. A time curve is then derived which is believed to be the resultant of a number of factors in serum,3 among which are ascorbic acid concentration, ceruloplasmin activity, and certain sulfhydryl compounds. (A typical curve is shown in Figure 1.) The lag period before the initial References 1. Akerfeldt, S.: Oxidation of N,N-Dimethyl-p-Phenylenediamine by Serum from Patients with Mental Disease , Science 125:117, 1957.Crossref 2. Abood, L.: A Comparative Study of Various Oxidases in the Serum of Schizophrenia and Other Diseases: Blood Tests in Mental Illness , Conference of the Brain Research Foundation, Chicago, 1957. 3. Akerfeldt, S.: Serological Reaction of Psychiatric Patients to PPD (N,N-Dimethyl-p-Phenylenediamine) , paper read at the annual meeting of the American Psychiatric Association, Adolf Meyer Research Lecture, Chicago, 1957. 4. Humoller, F. L.; Majka, F. A.; Barak, A. J.; Stevens, J. D., and Holthaus, J. M.: Determination of Plasma Amine Oxidase Activity , Clin. Chem. 4:1, 1958. 5. Friedhoff, A. J.: Unpublished data. 6. Holmberg, C. G., and Laurell, C. B.: Investigations in Serum Copper: IV. Effect of Different Anions on the Enzymatic Activity of Coeruloplasmin , Acta chem. scandinav. 5:921, 1951.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives of Neurology & Psychiatry American Medical Association

An Effect of Exercise, Skin Shock, and Ascorbic Acid on Serum Oxidase Activity

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Publisher
American Medical Association
Copyright
Copyright © 1959 American Medical Association. All Rights Reserved.
ISSN
0096-6886
DOI
10.1001/archneurpsyc.1959.02340170086009
Publisher site
See Article on Publisher Site

Abstract

Abstract In 1957 Akerfeldt1 reported that when the N,N-dimethyl form of p-phenylenediamine (PPD) is added to serum the PPD becomes oxidized to a red-colored substance. He found that serum from schizophrenic patients oxidized PPD at a faster rate than serum from normal controls. A rapid rate of oxidation was also found in a large percentage of patients with liver disease, neoplasm, and pregnancy in the last trimester.2 In this reaction a 0.1% solution of N,N-dimethyl-p-phenylenediamine is added to an equal quantity of serum. The increase in optical density is then measured spectrophotometrically for six minutes at wavelength 552 mμ. A time curve is then derived which is believed to be the resultant of a number of factors in serum,3 among which are ascorbic acid concentration, ceruloplasmin activity, and certain sulfhydryl compounds. (A typical curve is shown in Figure 1.) The lag period before the initial References 1. Akerfeldt, S.: Oxidation of N,N-Dimethyl-p-Phenylenediamine by Serum from Patients with Mental Disease , Science 125:117, 1957.Crossref 2. Abood, L.: A Comparative Study of Various Oxidases in the Serum of Schizophrenia and Other Diseases: Blood Tests in Mental Illness , Conference of the Brain Research Foundation, Chicago, 1957. 3. Akerfeldt, S.: Serological Reaction of Psychiatric Patients to PPD (N,N-Dimethyl-p-Phenylenediamine) , paper read at the annual meeting of the American Psychiatric Association, Adolf Meyer Research Lecture, Chicago, 1957. 4. Humoller, F. L.; Majka, F. A.; Barak, A. J.; Stevens, J. D., and Holthaus, J. M.: Determination of Plasma Amine Oxidase Activity , Clin. Chem. 4:1, 1958. 5. Friedhoff, A. J.: Unpublished data. 6. Holmberg, C. G., and Laurell, C. B.: Investigations in Serum Copper: IV. Effect of Different Anions on the Enzymatic Activity of Coeruloplasmin , Acta chem. scandinav. 5:921, 1951.Crossref

Journal

A.M.A. Archives of Neurology & PsychiatryAmerican Medical Association

Published: May 1, 1959

References