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Amylase and Lipase Secretion in the Hepatic and Intestinal Lymph: II. Progressive Changes in Enzyme Levels Following Pancreatectomy

Amylase and Lipase Secretion in the Hepatic and Intestinal Lymph: II. Progressive Changes in... Abstract Many previous studies have shown that pancreatectomy fails to deplete blood of its amylase and lipase activity. The maintenance of blood amylase and lipase activity following removal of the pancreas in the dog and in man suggests that nonpancreatic sources of these enzymes contribute significantly to the maintenance of normal blood levels. In our previous series of experiments, acute pancreatectomy did not cause the immediate disappearance of amylase and lipase from the lymph or serum. To further investigate the long-term effect of pancreatectomy on these enzymes, the present series of experiments was designed, in which hepatic and intestinal lymphatics of previously pancreatectomized dogs were cannulated and amylase and lipase levels were measured in the lymph and serum. Materials and Methods Mongrel dogs of either sex, weighing 10 to 20 kg, were subjected to laparotomy under pentobarbital sodium anesthesia. Sterile technique was utilized. Control samples of peripheral venous blood were taken References 1. Singh, H., et al: Amylase and Lipase Secretion in the Hepatic and Intestinal Lymph: I. Studies Involving Acute Pancreatectomy , Ann Surg 169:233-239 ( (Feb) ) 1969.Crossref 2. Cherry, I.S., and Crandall, L.A., Jr.: The Specificity of Pancreatic Lipase: Its Appearance in the Blood After Pancreatic Injury , Amer J Physiol 100:266-273 ( (April) ) 1932. 3. Somogyi, M.: Blood Diastase as an Indicator of Liver Function , Proc Soc Exp Biol Med 32:538-540 ( (Dec) ) 1934.Crossref 4. Carlson, A.J., and Luckhardt, A.B.: On the Diastases in the Blood and Body Fluids , Amer J Physiol 23:148-164 ( (Dec) ) 1908-1909. 5. Karsner, H.T.; Koeckert, H.L.; and Wahl, S.A.: The Diastatic Activity of the Blood in Experimental Hyperglycemia , J Exp Med 34:349-363 ( (Oct) ) 1921.Crossref 6. Reid, E.; Quigley, J.P.; and Myers, V.C.: Studies on Animal Diastases: Blood and Tissue Diastases With Special Reference to the Depancreatized Dogs , J Biol Chem 99:615-623 ( (Jan) ) 1932-1933. 7. Markowitz, J., and Hough, H.B.: A Study of Blood Diastases in Depancreatized Dogs , Amer J Physiol 75:571-578 ( (Feb) ) 1925-1926. 8. Zucker, T.F.; Newburger, P.G.; and Berg, B.N.: The Amylase of Serum in Relation to Functional States of the Pancreas , Amer J Physiol 102:209-221 ( (Oct) ) 1932. 9. Friedman, I., and Thompson, W.R.: Induced and Spontaneous Changes in Blood Amylase, Particularly in Relationship to the Pancreas , Ann Surg 104:388-402 ( (Sept) ) 1936.Crossref 10. Reid, C., and Narayana, B.: Studies in Blood Diastase: Factors Which Cause Variations in the Amount of Diastase in the Blood , Quart J Exp Physiol 20:305-311 ( (Aug) ) 1930. 11. Burton, T.Y.; Kearns, T.P.; and Rynearson, E.H.: Diabetic Retinopathy Following Total Pancreatectomy , Mayo Clin Proc 32:735-739 ( (Dec 25) ) 1957. 12. Nardi, G.L.: Metabolic Studies Following Total Pancreatectomy for Retroperitoneal Leomyosarcoma , New Eng J Med 247:548-550 ( (Oct 9) ) 1952.Crossref 13. Brunschwig, A.; Ricketts, H.T.; and Bigelow, R.R.: Total Pancreatectomy, Total Gastrectomy, Total Duodenectomy, Splenectomy, Left Adrenalectomy, and Omentectomy in a Diabetic Patient, Recovery , Surg Gynec Obstet 80:252-256 ( (March) ) 1945. 14. Schlesinger, W.: Ueber den Ursprung des diastatischen Fermentes im Blute und über seine Beziehungen znm Diabetes mellitus , Deutsch Med Wschr 34:593-595 ( (May) ) 1908.Crossref 15. Carlson, A. J., and Ryan, J.G.: The Diastase in Cat's Saliva , Amer J Physiol 22:1-15 ( (June 1) ) 1908. 16. Mendel, L.D., and Underhill, R.P.: Is the Saliva of the Dog Amylactically Active , J Biol Chem 3:135-143 ( (March 19) ) 1907. 17. Rachmilewitz, M.: Blood Diastase in Hepatic and Biliary Disease , Amer J Dig Dis 5:184-189 ( (July) ) 1938.Crossref 18. Gray, S.H.; Probstein, J.G.; Heifetz, C.J.: Clinical Studies on Blood Diastase: I. Low Blood Diastase as an Index of Impaired Hepatic Function , Arch Intern Med 67:805-818 ( (April) ) 1941.Crossref 19. Lewison, E.F.: The Clinical Value of the Serum Amylase Test , Surg Gynec Obstet 72:202-212 ( (Feb) ) 1941. 20. Cajori, F.A., and Vars, H.M.: The Effect of Chloroform Anesthesia on Serum Amylase and Liver Esterase , Amer J Physiol 124:149-154 ( (Oct) ) 1938. 21. Roe, J.H., and Goldstein, N.P.: Studies of Pancreatic Function: The Effect of Injury to the Pancreas or the Liver Upon the Amylase and Lipase Content of the Blood , J Lab Clin Med 28:1334-1344 ( (Aug) ) 1943. 22. Davis, L.H., and Ross, E.L.: The Sources of Diastases of the Blood , Amer J Physiol 56:22-28 ( (May 1) ) 1921. 23. Flock, E.V., et al: Alkaline Phosphate and Amylase of Plasma After Hepatectomy , Amer J Physiol 170:467-471 ( (Aug) ) 1952. 24. Khattab, M., et al: Hepatic Influence on Amylase of Plasma , Amer J Physiol 175:458-460 ( (Dec) ) 1953. 25. Hiatt, N.: Observations of the Blood Amylase Level in the Dog With Experimentally Produced Simple and Strangulated Obstructions of the Small Intestine , Ann Surg 149:77-86 ( (Jan) ) 1959.Crossref 26. Nothman, M.M.; Pratt, T.D.; and Benotti, J.: The Effect of the Ligation of the Pancreatic Ducts and of Pancreatectomy After Duct Ligation on Serum Lipase , J Lab Clin Med 33:833-840 ( (Jan) ) 1948. 27. Goldstein, N.P., et al: Studies of Pancreatic Function: The Effect of Ligation of the Pancreatic Ducts Upon the Amylase and Lipase Content of the Blood , J Lab Clin Med 31:999-1003 ( (Sept) ) 1946. 28. Gomori, G.: Histochemical Localization of True Lipase , Proc Soc Exp Biol Med 72:697-700 ( (Dec) ) 1949.Crossref 29. Muneaki, A.; Kramer, S.P.; and Seligman, A.M.: The Histochemical Demonstration of Pancreatic-Like Lipase and Comparison With the Distribution of Esterase , J Histo Chem Cyto Chem 12:364-383 ( (May) ) 1964.Crossref 30. Jobling, J.W.; Eggstein, A.A.; and Petersen, W.: The Relation of Serum Esterase to Liver Destruction , J Exp Med 22:701-706 ( (Dec 1) ) 1915.Crossref 31. Boldyreff, W.N.: Die Lipase des Darmsaftes und ihre Charakteristik , Hoppe Seyler Z Physiol Chem 50:394, 1912.Crossref 32. Schiff, M., quoted by Desnuelle, P., et al: Arch Phys Norm Path 4:699, 1892. 33. Desnuelle, P., and Savary, P.: Specificities of Lipases , J Lipid Res 4:369-384 (Oct) 1963. 34. Hagony, P.L.; Vekerdi, L.; and Keleti, T.: Studies on Lipase Activity in Rat Tissues , Enzymologia 23:321-326, 1961. 35. Senior, J.R., and Isselbacher, K.J.: Demonstration of an Intestinal Monoglyceride Lipase: An Enzyme With a Possible Role in the Intracellular Completion of Fat Digestion , J Clin Invest 42:187-195 ( (Feb) ) 1963.Crossref 36. Kataeva, G.A., and Filin, V.I.: Secretory and Enzymatic Functions of a Denervated Segment of the Human Small Intestine: Federation Proceedings (Translation Supplement) 23:5-6 (Jan) 1964. 37. Appert, H.E., et al: Studies on the Disappearance of Pancreatic Enzymes Following Intravenous Injection of Pancreatic Juice in the Dog , Surg Gynec Obstet 127:1281-1287 ( (Dec) ) 1968. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Amylase and Lipase Secretion in the Hepatic and Intestinal Lymph: II. Progressive Changes in Enzyme Levels Following Pancreatectomy

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Publisher
American Medical Association
Copyright
Copyright © 1969 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1969.01340130082015
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Abstract

Abstract Many previous studies have shown that pancreatectomy fails to deplete blood of its amylase and lipase activity. The maintenance of blood amylase and lipase activity following removal of the pancreas in the dog and in man suggests that nonpancreatic sources of these enzymes contribute significantly to the maintenance of normal blood levels. In our previous series of experiments, acute pancreatectomy did not cause the immediate disappearance of amylase and lipase from the lymph or serum. To further investigate the long-term effect of pancreatectomy on these enzymes, the present series of experiments was designed, in which hepatic and intestinal lymphatics of previously pancreatectomized dogs were cannulated and amylase and lipase levels were measured in the lymph and serum. Materials and Methods Mongrel dogs of either sex, weighing 10 to 20 kg, were subjected to laparotomy under pentobarbital sodium anesthesia. Sterile technique was utilized. Control samples of peripheral venous blood were taken References 1. Singh, H., et al: Amylase and Lipase Secretion in the Hepatic and Intestinal Lymph: I. Studies Involving Acute Pancreatectomy , Ann Surg 169:233-239 ( (Feb) ) 1969.Crossref 2. Cherry, I.S., and Crandall, L.A., Jr.: The Specificity of Pancreatic Lipase: Its Appearance in the Blood After Pancreatic Injury , Amer J Physiol 100:266-273 ( (April) ) 1932. 3. Somogyi, M.: Blood Diastase as an Indicator of Liver Function , Proc Soc Exp Biol Med 32:538-540 ( (Dec) ) 1934.Crossref 4. Carlson, A.J., and Luckhardt, A.B.: On the Diastases in the Blood and Body Fluids , Amer J Physiol 23:148-164 ( (Dec) ) 1908-1909. 5. Karsner, H.T.; Koeckert, H.L.; and Wahl, S.A.: The Diastatic Activity of the Blood in Experimental Hyperglycemia , J Exp Med 34:349-363 ( (Oct) ) 1921.Crossref 6. Reid, E.; Quigley, J.P.; and Myers, V.C.: Studies on Animal Diastases: Blood and Tissue Diastases With Special Reference to the Depancreatized Dogs , J Biol Chem 99:615-623 ( (Jan) ) 1932-1933. 7. Markowitz, J., and Hough, H.B.: A Study of Blood Diastases in Depancreatized Dogs , Amer J Physiol 75:571-578 ( (Feb) ) 1925-1926. 8. Zucker, T.F.; Newburger, P.G.; and Berg, B.N.: The Amylase of Serum in Relation to Functional States of the Pancreas , Amer J Physiol 102:209-221 ( (Oct) ) 1932. 9. Friedman, I., and Thompson, W.R.: Induced and Spontaneous Changes in Blood Amylase, Particularly in Relationship to the Pancreas , Ann Surg 104:388-402 ( (Sept) ) 1936.Crossref 10. Reid, C., and Narayana, B.: Studies in Blood Diastase: Factors Which Cause Variations in the Amount of Diastase in the Blood , Quart J Exp Physiol 20:305-311 ( (Aug) ) 1930. 11. Burton, T.Y.; Kearns, T.P.; and Rynearson, E.H.: Diabetic Retinopathy Following Total Pancreatectomy , Mayo Clin Proc 32:735-739 ( (Dec 25) ) 1957. 12. Nardi, G.L.: Metabolic Studies Following Total Pancreatectomy for Retroperitoneal Leomyosarcoma , New Eng J Med 247:548-550 ( (Oct 9) ) 1952.Crossref 13. Brunschwig, A.; Ricketts, H.T.; and Bigelow, R.R.: Total Pancreatectomy, Total Gastrectomy, Total Duodenectomy, Splenectomy, Left Adrenalectomy, and Omentectomy in a Diabetic Patient, Recovery , Surg Gynec Obstet 80:252-256 ( (March) ) 1945. 14. Schlesinger, W.: Ueber den Ursprung des diastatischen Fermentes im Blute und über seine Beziehungen znm Diabetes mellitus , Deutsch Med Wschr 34:593-595 ( (May) ) 1908.Crossref 15. Carlson, A. J., and Ryan, J.G.: The Diastase in Cat's Saliva , Amer J Physiol 22:1-15 ( (June 1) ) 1908. 16. Mendel, L.D., and Underhill, R.P.: Is the Saliva of the Dog Amylactically Active , J Biol Chem 3:135-143 ( (March 19) ) 1907. 17. Rachmilewitz, M.: Blood Diastase in Hepatic and Biliary Disease , Amer J Dig Dis 5:184-189 ( (July) ) 1938.Crossref 18. Gray, S.H.; Probstein, J.G.; Heifetz, C.J.: Clinical Studies on Blood Diastase: I. Low Blood Diastase as an Index of Impaired Hepatic Function , Arch Intern Med 67:805-818 ( (April) ) 1941.Crossref 19. Lewison, E.F.: The Clinical Value of the Serum Amylase Test , Surg Gynec Obstet 72:202-212 ( (Feb) ) 1941. 20. Cajori, F.A., and Vars, H.M.: The Effect of Chloroform Anesthesia on Serum Amylase and Liver Esterase , Amer J Physiol 124:149-154 ( (Oct) ) 1938. 21. Roe, J.H., and Goldstein, N.P.: Studies of Pancreatic Function: The Effect of Injury to the Pancreas or the Liver Upon the Amylase and Lipase Content of the Blood , J Lab Clin Med 28:1334-1344 ( (Aug) ) 1943. 22. Davis, L.H., and Ross, E.L.: The Sources of Diastases of the Blood , Amer J Physiol 56:22-28 ( (May 1) ) 1921. 23. Flock, E.V., et al: Alkaline Phosphate and Amylase of Plasma After Hepatectomy , Amer J Physiol 170:467-471 ( (Aug) ) 1952. 24. Khattab, M., et al: Hepatic Influence on Amylase of Plasma , Amer J Physiol 175:458-460 ( (Dec) ) 1953. 25. Hiatt, N.: Observations of the Blood Amylase Level in the Dog With Experimentally Produced Simple and Strangulated Obstructions of the Small Intestine , Ann Surg 149:77-86 ( (Jan) ) 1959.Crossref 26. Nothman, M.M.; Pratt, T.D.; and Benotti, J.: The Effect of the Ligation of the Pancreatic Ducts and of Pancreatectomy After Duct Ligation on Serum Lipase , J Lab Clin Med 33:833-840 ( (Jan) ) 1948. 27. Goldstein, N.P., et al: Studies of Pancreatic Function: The Effect of Ligation of the Pancreatic Ducts Upon the Amylase and Lipase Content of the Blood , J Lab Clin Med 31:999-1003 ( (Sept) ) 1946. 28. Gomori, G.: Histochemical Localization of True Lipase , Proc Soc Exp Biol Med 72:697-700 ( (Dec) ) 1949.Crossref 29. Muneaki, A.; Kramer, S.P.; and Seligman, A.M.: The Histochemical Demonstration of Pancreatic-Like Lipase and Comparison With the Distribution of Esterase , J Histo Chem Cyto Chem 12:364-383 ( (May) ) 1964.Crossref 30. Jobling, J.W.; Eggstein, A.A.; and Petersen, W.: The Relation of Serum Esterase to Liver Destruction , J Exp Med 22:701-706 ( (Dec 1) ) 1915.Crossref 31. Boldyreff, W.N.: Die Lipase des Darmsaftes und ihre Charakteristik , Hoppe Seyler Z Physiol Chem 50:394, 1912.Crossref 32. Schiff, M., quoted by Desnuelle, P., et al: Arch Phys Norm Path 4:699, 1892. 33. Desnuelle, P., and Savary, P.: Specificities of Lipases , J Lipid Res 4:369-384 (Oct) 1963. 34. Hagony, P.L.; Vekerdi, L.; and Keleti, T.: Studies on Lipase Activity in Rat Tissues , Enzymologia 23:321-326, 1961. 35. Senior, J.R., and Isselbacher, K.J.: Demonstration of an Intestinal Monoglyceride Lipase: An Enzyme With a Possible Role in the Intracellular Completion of Fat Digestion , J Clin Invest 42:187-195 ( (Feb) ) 1963.Crossref 36. Kataeva, G.A., and Filin, V.I.: Secretory and Enzymatic Functions of a Denervated Segment of the Human Small Intestine: Federation Proceedings (Translation Supplement) 23:5-6 (Jan) 1964. 37. Appert, H.E., et al: Studies on the Disappearance of Pancreatic Enzymes Following Intravenous Injection of Pancreatic Juice in the Dog , Surg Gynec Obstet 127:1281-1287 ( (Dec) ) 1968.

Journal

Archives of Surgery – American Medical Association

Published: Jul 1, 1969

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