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Alterations in Lipogenic Enzymes and Lipoprotein Lipase Activity During Gram-Negative Sepsis in the Rat

Alterations in Lipogenic Enzymes and Lipoprotein Lipase Activity During Gram-Negative Sepsis in... Abstract • The effects of sepsis on lipid metabolism have not been clearly defined. This study was designed to observe the changes in adipose tissue lipoprotein lipase (LPL) and fatty acid synthetase (FAS) after administration of Escherichia coli bacteria. Male Lewis rats, weighing 245 to 270 g, were assigned to two groups and fed a powdered chow diet for 14 days. On day 14, one group was inoculated with E coli. Twenty-four hours later, both groups were killed by decapitation. Serum triglyceride levels were significantly elevated in the E coli–treated rats. Adipose tissue LPL and FAS activity was significantly decreased by 50% in E coli–treated rats compared with the control rats. These results suggest that the elevated serum triglyceride levels associated with sepsis may be caused by a decreased rate of clearance of lipids from the blood and an increased rate of hepatic lipid synthesis. (Arch Surg 1982;117:144-147) References 1. Gallin JI, Kaye D, O'Leary WM: Serum lipids in infection . N Engl J Med 1969;281:1081-1086.Crossref 2. Hirsch RL, McKay DG, Travers RI, et al: Hyperlipidemia, fatty liver, and bromsulfophthalein retention in rabbits injected with bacterial endotoxins . J Lipid Res 1964;5:563-568. 3. Bagby G, Spitzer JA: Lipoprotein lipase activity in rat heart and adipose tissue during endotoxic shock . Am J Physiol 1980;238:325-330. 4. Pace JG, Murphy PE, Wannemacher RW: Infection-related changes in enzymes of hepatic fatty acid metabolism . Fed Proc 1981;40:338. 5. Wichterman KA, Baue AE, Chaudry IH: Sepsis and septic shock: A review of laboratory models and a proposal . J Surg Res 1980;29:189-201.Crossref 6. Trinder P: Determination of blood glucose using 4-amino phenazone as oxygen acceptor . J Clin Pathol 1969;22:246.Crossref 7. Bucolo G, David H: Quantitative determination of serum triglycerides by the use of enzymes . Clin Chem 1973;19:476-482. 8. Chianori N, Henry RJ: Study of ferric chloride method for determination of total cholesterol and total cholesterol esters . Am J Clin Pathol 1959;31:305-309. 9. Williamson DH, Mellanby J, Krebs HA: Enzymatic determination of D(−)-β-hydroxybutyric acid and acetonacetic acid in blood . Biochem J 1962;82:90-96. 10. Berde CB, Kerner JA, Johnson JD: Use of the conjugated polyene fatty acid, parinaric acid, in assaying fatty acids in serum or plasma . Clin Chem 1980;26:1173-1177. 11. Bradford MM: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anal Biochem 1976;72:248-254.Crossref 12. Roehrig KL, Albred JB: Direct enzymatic procedure for the determination of liver glycogen . Anal Biochem 1974;58:411-421.Crossref 13. Folch J, Lees M, Sloane Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues . J Biochem 1957;226:497-509. 14. Milner JA: Mechanism for fatty liver induction in rats fed arginase deficient diets . J Nutr 1979;190:663-670. 15. Hsu RY, Butterworth RHW, Porter SW: Pigeon liver fatty acid synthetase . Methods Enzymol 1969;14:33-34. 16. Takedo Y, Suzuki F, Inoue H: ATP citrate lyase (citrate cleavage enzyme) . Methods Enzymol 1969;13:153-160. 17. Schotz M, Garfinkel A, Auebolter RJ, et al: A rapid assay for lipoprotein lipase . J Lipid Res 1970;11:68-69. 18. Hietanen E, Greenwood MRC: A comparison of lipoprotein lipase activity and adipocyte differentiation in growing male rats . J Lipid Res 1977;18:480-490. 19. Zar JH: Biostatistical Analysis . Englewood Cliffs, NJ, Prentice-Hall Inc, 1974. 20. Wannemacher RW, Pace JG, Beall FA et al: Role of the liver in regulation of ketone body production during sepsis . J Clin Invest 1979;64:1565-1572.Crossref 21. Kaufmann RL, Matson CF, Beisel WR: Hypertriglyceridemia produced by endotoxin: Role of impaired triglyceride disposal mechanisms . J Infect Dis 1976;133:548-555.Crossref 22. Spitzer JJ: Lipid metabolism in endotoxin shock . Circ Shock Suppl 1979;1:69-79. 23. De Gasquet P, Gringlio S, Pequignot-Planche E, et al: Diurnal changes in plasma and liver and lipoprotein lipase activity in heart and adipose tissue in rats fed a high and low fat diet . J Nutr 1977;107:199-212. 24. Delorme CLW, Harris KL: Effects of diet on lipoprotein lipase activity in the rat . J Nutr 1975;105:447-451. 25. Ruth EA, Hems DA, Beloff-Chain A: Lipoprotein lipase activities in tissues of normal and genetically obese (ob/ob) mice . Diabetologia 1974;10:261-265. 26. De Gasquet P, Pequignot E: Adipose tissue lipoprotein lipase activity and cellularity in the genetically obese Zucker rat (fa/fa) . Biochem J 1973;132:633-635. 27. Gruen R, Hiatanen E, Greenwood MRC: Increased adipose tissue lipoprotein lipase activity during development of the genetically obese rats (fa-fa) . Metabolism 1978;26:1955-1965.Crossref 28. Spooner PM, Garrison MM, Siow RO: Regulation of mammary and adipose tissue lipoprotein lipase and blood triacylglycerol in rats during late pregnancy . J Clin Invest 1977;60:702-708.Crossref 29. Hamosh M, Clary TR, Chernick SA, et al: Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats . Biochim Biophys Acta 1970;210:473-482.Crossref 30. Wilson DE, Flowers CM, Carlili SI, et al: Estrogen treatment and gonadal function in the regulation of lipoprotein lipase activity . Artherosclerosis 1976;24:491-499.Crossref 31. Steingrimsdotter L, Diasil J, Greenwood MRC: Hormonal modulation of adipose tissue lipoprotein lipase may alter food intake in rats . Am J Physiol 1980;239:162-167. 32. Taskinen MR, Nikkila EA: Effects of caloric restriction on lipid metabolism in man . Atherosclerosis 1979;32:289-299.Crossref 33. Canonico PG, Ayala E, Rill WL: Effects of pneumococcal infection on rat liver microsomal enzymes and lipogenesis by isolated hepatocytes . Am J Clin Nutr 1977;30:1359-1363. 34. Foster DW, Srere PA: Citrate cleavage enzyme and fatty acid synthesis . J Biol Chem 1968;243:1926-1930. 35. Griffiths J, Groves AC, Leung FYT: The relationship of plasma catecholamines to serum trigylcerides in canine gram-negative bacteremia . Surg Gynecol Obstet 1973;134:795-798. 36. Duff JH, Finley RJ, Marchuk JB, et al: Catabolic hormones in sepsis . Clin Res 1975;23:635. 37. Feigelson EB, Pfaff WW, Karmen A, et al: The role of plasma free fatty acids in the development of fatty liver . J Clin Invest 1971;40:2171-2180.Crossref 38. Griffiths J, Groves AC, Leung FY: Hypertriglyceridemia and hypoglycemia in gram-negative sepsis in the dog . Surg Gynecol Obstet 1973;136:897-903. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Alterations in Lipogenic Enzymes and Lipoprotein Lipase Activity During Gram-Negative Sepsis in the Rat

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Publisher
American Medical Association
Copyright
Copyright © 1982 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1982.01380260028005
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Abstract

Abstract • The effects of sepsis on lipid metabolism have not been clearly defined. This study was designed to observe the changes in adipose tissue lipoprotein lipase (LPL) and fatty acid synthetase (FAS) after administration of Escherichia coli bacteria. Male Lewis rats, weighing 245 to 270 g, were assigned to two groups and fed a powdered chow diet for 14 days. On day 14, one group was inoculated with E coli. Twenty-four hours later, both groups were killed by decapitation. Serum triglyceride levels were significantly elevated in the E coli–treated rats. Adipose tissue LPL and FAS activity was significantly decreased by 50% in E coli–treated rats compared with the control rats. These results suggest that the elevated serum triglyceride levels associated with sepsis may be caused by a decreased rate of clearance of lipids from the blood and an increased rate of hepatic lipid synthesis. (Arch Surg 1982;117:144-147) References 1. Gallin JI, Kaye D, O'Leary WM: Serum lipids in infection . N Engl J Med 1969;281:1081-1086.Crossref 2. Hirsch RL, McKay DG, Travers RI, et al: Hyperlipidemia, fatty liver, and bromsulfophthalein retention in rabbits injected with bacterial endotoxins . J Lipid Res 1964;5:563-568. 3. Bagby G, Spitzer JA: Lipoprotein lipase activity in rat heart and adipose tissue during endotoxic shock . Am J Physiol 1980;238:325-330. 4. Pace JG, Murphy PE, Wannemacher RW: Infection-related changes in enzymes of hepatic fatty acid metabolism . Fed Proc 1981;40:338. 5. Wichterman KA, Baue AE, Chaudry IH: Sepsis and septic shock: A review of laboratory models and a proposal . J Surg Res 1980;29:189-201.Crossref 6. Trinder P: Determination of blood glucose using 4-amino phenazone as oxygen acceptor . J Clin Pathol 1969;22:246.Crossref 7. Bucolo G, David H: Quantitative determination of serum triglycerides by the use of enzymes . Clin Chem 1973;19:476-482. 8. Chianori N, Henry RJ: Study of ferric chloride method for determination of total cholesterol and total cholesterol esters . Am J Clin Pathol 1959;31:305-309. 9. Williamson DH, Mellanby J, Krebs HA: Enzymatic determination of D(−)-β-hydroxybutyric acid and acetonacetic acid in blood . Biochem J 1962;82:90-96. 10. Berde CB, Kerner JA, Johnson JD: Use of the conjugated polyene fatty acid, parinaric acid, in assaying fatty acids in serum or plasma . Clin Chem 1980;26:1173-1177. 11. Bradford MM: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anal Biochem 1976;72:248-254.Crossref 12. Roehrig KL, Albred JB: Direct enzymatic procedure for the determination of liver glycogen . Anal Biochem 1974;58:411-421.Crossref 13. Folch J, Lees M, Sloane Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues . J Biochem 1957;226:497-509. 14. Milner JA: Mechanism for fatty liver induction in rats fed arginase deficient diets . J Nutr 1979;190:663-670. 15. Hsu RY, Butterworth RHW, Porter SW: Pigeon liver fatty acid synthetase . Methods Enzymol 1969;14:33-34. 16. Takedo Y, Suzuki F, Inoue H: ATP citrate lyase (citrate cleavage enzyme) . Methods Enzymol 1969;13:153-160. 17. Schotz M, Garfinkel A, Auebolter RJ, et al: A rapid assay for lipoprotein lipase . J Lipid Res 1970;11:68-69. 18. Hietanen E, Greenwood MRC: A comparison of lipoprotein lipase activity and adipocyte differentiation in growing male rats . J Lipid Res 1977;18:480-490. 19. Zar JH: Biostatistical Analysis . Englewood Cliffs, NJ, Prentice-Hall Inc, 1974. 20. Wannemacher RW, Pace JG, Beall FA et al: Role of the liver in regulation of ketone body production during sepsis . J Clin Invest 1979;64:1565-1572.Crossref 21. Kaufmann RL, Matson CF, Beisel WR: Hypertriglyceridemia produced by endotoxin: Role of impaired triglyceride disposal mechanisms . J Infect Dis 1976;133:548-555.Crossref 22. Spitzer JJ: Lipid metabolism in endotoxin shock . Circ Shock Suppl 1979;1:69-79. 23. De Gasquet P, Gringlio S, Pequignot-Planche E, et al: Diurnal changes in plasma and liver and lipoprotein lipase activity in heart and adipose tissue in rats fed a high and low fat diet . J Nutr 1977;107:199-212. 24. Delorme CLW, Harris KL: Effects of diet on lipoprotein lipase activity in the rat . J Nutr 1975;105:447-451. 25. Ruth EA, Hems DA, Beloff-Chain A: Lipoprotein lipase activities in tissues of normal and genetically obese (ob/ob) mice . Diabetologia 1974;10:261-265. 26. De Gasquet P, Pequignot E: Adipose tissue lipoprotein lipase activity and cellularity in the genetically obese Zucker rat (fa/fa) . Biochem J 1973;132:633-635. 27. Gruen R, Hiatanen E, Greenwood MRC: Increased adipose tissue lipoprotein lipase activity during development of the genetically obese rats (fa-fa) . Metabolism 1978;26:1955-1965.Crossref 28. Spooner PM, Garrison MM, Siow RO: Regulation of mammary and adipose tissue lipoprotein lipase and blood triacylglycerol in rats during late pregnancy . J Clin Invest 1977;60:702-708.Crossref 29. Hamosh M, Clary TR, Chernick SA, et al: Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats . Biochim Biophys Acta 1970;210:473-482.Crossref 30. Wilson DE, Flowers CM, Carlili SI, et al: Estrogen treatment and gonadal function in the regulation of lipoprotein lipase activity . Artherosclerosis 1976;24:491-499.Crossref 31. Steingrimsdotter L, Diasil J, Greenwood MRC: Hormonal modulation of adipose tissue lipoprotein lipase may alter food intake in rats . Am J Physiol 1980;239:162-167. 32. Taskinen MR, Nikkila EA: Effects of caloric restriction on lipid metabolism in man . Atherosclerosis 1979;32:289-299.Crossref 33. Canonico PG, Ayala E, Rill WL: Effects of pneumococcal infection on rat liver microsomal enzymes and lipogenesis by isolated hepatocytes . Am J Clin Nutr 1977;30:1359-1363. 34. Foster DW, Srere PA: Citrate cleavage enzyme and fatty acid synthesis . J Biol Chem 1968;243:1926-1930. 35. Griffiths J, Groves AC, Leung FYT: The relationship of plasma catecholamines to serum trigylcerides in canine gram-negative bacteremia . Surg Gynecol Obstet 1973;134:795-798. 36. Duff JH, Finley RJ, Marchuk JB, et al: Catabolic hormones in sepsis . Clin Res 1975;23:635. 37. Feigelson EB, Pfaff WW, Karmen A, et al: The role of plasma free fatty acids in the development of fatty liver . J Clin Invest 1971;40:2171-2180.Crossref 38. Griffiths J, Groves AC, Leung FY: Hypertriglyceridemia and hypoglycemia in gram-negative sepsis in the dog . Surg Gynecol Obstet 1973;136:897-903.

Journal

Archives of SurgeryAmerican Medical Association

Published: Feb 1, 1982

References