Abstract • Interleukin 1 has been implicated in intraocular inflammation. The availability of a cloned, recombinant interleukin 1 receptor antagonist has enabled us to test the role of interleukin 1 in specific models of uveitis in New Zealand white rabbits. Seventy-five micrograms of interleukin 1 receptor antagonist injected intravitreally resulted in a 97% reduction in aqueous humor cells present 6 hours after intravitreal injection of 10 ng of human interleukin 1 α. Disruption of the blood aqueous barrier was prevented by the receptor antagonist (mean SD aqueous humor protein of 0.6±0.1 g/L in rabbits treated with interleukin 1 receptor antagonist vs 32.2±9.9 g/L in controls). Lower doses of interleukin 1 produced more modest but significant inhibition. 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The effect of an interleukin-1 receptor antagonist protein on type II collagen and antigen-induced arthritis in mice . Arthritis Rheum . 1990;33:S20. 18. Rosenbaum JT, Raymond W. Monocyte chemotactic activity induced by intravitreal endotoxin . Invest Ophthalmol Vis Sci . 1985;26:1267-1273. 19. Rosenbaum JT, Seymour BWP, Raymond W, Langlois L. Wu M, David L. A similar chemotactic factor for monocytes predominates in different animal models of uveitis . Inflammation . 1988;12:191-201.Crossref 20. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anal Biochem . 1976;72:248-254.Crossref 21. Dinarello CA. Biology of interleukin 1 . FASEB J . 1988;2:108-115. 22. Cominelli F, Nast CC, Clark BD, et al. Interleukin 1 (IL-1) gene expression, synthesis, and effect of specific IL-1 receptor blockade in rabbit immune complex colitis . J Clin Invest . 1990;86:972-980.Crossref 23. 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Archives of Ophthalmology – American Medical Association
Published: Apr 1, 1992
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