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A DISTURBING FACTOR IN LIEBEN'S AND IN GUNNING'S TEST FOR ACETONE IN URINE

A DISTURBING FACTOR IN LIEBEN'S AND IN GUNNING'S TEST FOR ACETONE IN URINE Lately, while testing a urine for acetone by means of Lieben's and also Gunning's test, I found both tests to be negative when applied to the urine direct,but positive when applied to the distillate. Naturally, one would think that some substance was present in the urine interfering with the formation of iodoform, the reaction on which the above-mentioned tests are based. This urine gave a marked reaction for protein and it was found that it was the presence of this substance which interfered with the above tests on account of the fact that after removal of the protein by means of dilute acetic acid, the urine responded to Lieben's and to Gunning's test.1 It is well recognized that only the distillate of the acidified urine should be used for acetone tests, but many workers often apply Lieben's test to the urine direct and if it is found to be http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JAMA American Medical Association

A DISTURBING FACTOR IN LIEBEN'S AND IN GUNNING'S TEST FOR ACETONE IN URINE

JAMA , Volume LIX (6) – Aug 10, 1912

A DISTURBING FACTOR IN LIEBEN'S AND IN GUNNING'S TEST FOR ACETONE IN URINE

Abstract


Lately, while testing a urine for acetone by means of Lieben's and also Gunning's test, I found both tests to be negative when applied to the urine direct,but positive when applied to the distillate. Naturally, one would think that some substance was present in the urine interfering with the formation of iodoform, the reaction on which the above-mentioned tests are based.
This urine gave a marked reaction for protein and it was found that it was the presence of this substance which...
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Publisher
American Medical Association
Copyright
Copyright © 1912 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
ISSN
0098-7484
eISSN
1538-3598
DOI
10.1001/jama.1912.04270080127022
Publisher site
See Article on Publisher Site

Abstract

Lately, while testing a urine for acetone by means of Lieben's and also Gunning's test, I found both tests to be negative when applied to the urine direct,but positive when applied to the distillate. Naturally, one would think that some substance was present in the urine interfering with the formation of iodoform, the reaction on which the above-mentioned tests are based. This urine gave a marked reaction for protein and it was found that it was the presence of this substance which interfered with the above tests on account of the fact that after removal of the protein by means of dilute acetic acid, the urine responded to Lieben's and to Gunning's test.1 It is well recognized that only the distillate of the acidified urine should be used for acetone tests, but many workers often apply Lieben's test to the urine direct and if it is found to be

Journal

JAMAAmerican Medical Association

Published: Aug 10, 1912

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