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Experimental Autoimmune Encephalomyelitis in the Rat Spinal Cord: Lesion Detection with High-Resolution MR Microscopy at 17.6 T

Experimental Autoimmune Encephalomyelitis in the Rat Spinal Cord: Lesion Detection with... This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Steinbrecher, A. Articles by Faber, C. Search for Related Content PubMed PubMed Citation Articles by Steinbrecher, A. Articles by Faber, C. Hotlight (NEW!) What's Hotlight? American Journal of Neuroradiology 26:19-25, January 2005 © 2005 American Society of Neuroradiology SPINE Experimental Autoimmune Encephalomyelitis in the Rat Spinal Cord: Lesion Detection with High-Resolution MR Microscopy at 17.6 T Andreas Steinbrecher a , Thomas Weber b , Thomas Neuberger b , André M. Mueller a , Xiomara Pedré a , Gerhard Giegerich a , Ulrich Bogdahn a , Peter Jakob b , Axel Haase b and Cornelius Faber b a Department of Neurology, University of Regensburg, Germany b Germany and Physikalisches Institut, EP5, University of Wuerzburg, Germany Address correspondence to: Andreas Steinbrecher, MD, Department of Neurology, University of Regensburg, Universitaetsstr 84, D-93053 Regensburg, Germany BACKGROUND AND PURPOSE: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disorder of the CNS and an animal model of multiple sclerosis. We used high-field MR microscopy at 17.6 T to image spinal cord inflammatory lesions in the acute stage of chronic relapsing rat EAE. We sought to compare lesions detected on MR imaging with histopathologic findings and to quantify the inflammatory lesion load. METHODS: Imaging of fixed spinal cord specimens was performed by using a 3D gradient-echo sequence with a spatial resolution of 35 x 35 x 58 µm 3 and a total imaging time of 5.5 hours. Histopathologic analysis was performed by staining axial sections with hematoxylin-eosin or Luxol fast blue to identify cellular infiltration and demyelination. RESULTS: Clinical signs of EAE occurred on days 10–14 after immunization. On day 22, healthy white matter and gray matter were differentiated by high contrast on T2*-weighted images, with white matter lesions appearing as hyperintense areas in the normal-appearing white matter. Inflammatory lesions identified on histopathologic evaluation were readily detected with MR imaging and vice versa. MR imaging and histopathologic analysis had excellent correlation regarding the extent of white matter lesions. Inflammatory infiltrates of gray matter were not detectable with MR imaging. Using a semiautomatic segmentation of the acquired MR data, we could quantify white matter lesion load. CONCLUSION: Ex vivo high-resolution MR microscopy of the spinal cord at 17.6 T allows rapid and highly accurate determination of CNS inflammation by demonstrating virtually all histologically detectable white matter inflammatory lesions. Home Subscribe Author Instructions Submit Online Search the AJNR Archives Feedback Help Copyright © 2010 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Neuroradiology American Journal of Neuroradiology

Experimental Autoimmune Encephalomyelitis in the Rat Spinal Cord: Lesion Detection with High-Resolution MR Microscopy at 17.6 T

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Publisher
American Journal of Neuroradiology
Copyright
Copyright © 2010 by the American Society of Neuroradiology.
ISSN
0195-6108
eISSN
1936-959X
Publisher site
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Abstract

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Steinbrecher, A. Articles by Faber, C. Search for Related Content PubMed PubMed Citation Articles by Steinbrecher, A. Articles by Faber, C. Hotlight (NEW!) What's Hotlight? American Journal of Neuroradiology 26:19-25, January 2005 © 2005 American Society of Neuroradiology SPINE Experimental Autoimmune Encephalomyelitis in the Rat Spinal Cord: Lesion Detection with High-Resolution MR Microscopy at 17.6 T Andreas Steinbrecher a , Thomas Weber b , Thomas Neuberger b , André M. Mueller a , Xiomara Pedré a , Gerhard Giegerich a , Ulrich Bogdahn a , Peter Jakob b , Axel Haase b and Cornelius Faber b a Department of Neurology, University of Regensburg, Germany b Germany and Physikalisches Institut, EP5, University of Wuerzburg, Germany Address correspondence to: Andreas Steinbrecher, MD, Department of Neurology, University of Regensburg, Universitaetsstr 84, D-93053 Regensburg, Germany BACKGROUND AND PURPOSE: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disorder of the CNS and an animal model of multiple sclerosis. We used high-field MR microscopy at 17.6 T to image spinal cord inflammatory lesions in the acute stage of chronic relapsing rat EAE. We sought to compare lesions detected on MR imaging with histopathologic findings and to quantify the inflammatory lesion load. METHODS: Imaging of fixed spinal cord specimens was performed by using a 3D gradient-echo sequence with a spatial resolution of 35 x 35 x 58 µm 3 and a total imaging time of 5.5 hours. Histopathologic analysis was performed by staining axial sections with hematoxylin-eosin or Luxol fast blue to identify cellular infiltration and demyelination. RESULTS: Clinical signs of EAE occurred on days 10–14 after immunization. On day 22, healthy white matter and gray matter were differentiated by high contrast on T2*-weighted images, with white matter lesions appearing as hyperintense areas in the normal-appearing white matter. Inflammatory lesions identified on histopathologic evaluation were readily detected with MR imaging and vice versa. MR imaging and histopathologic analysis had excellent correlation regarding the extent of white matter lesions. Inflammatory infiltrates of gray matter were not detectable with MR imaging. Using a semiautomatic segmentation of the acquired MR data, we could quantify white matter lesion load. CONCLUSION: Ex vivo high-resolution MR microscopy of the spinal cord at 17.6 T allows rapid and highly accurate determination of CNS inflammation by demonstrating virtually all histologically detectable white matter inflammatory lesions. Home Subscribe Author Instructions Submit Online Search the AJNR Archives Feedback Help Copyright © 2010 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X

Journal

American Journal of Neuroradiology – American Journal of Neuroradiology

Published: Jan 1, 2005

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