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Endovascular Treatment of Experimental Aneurysms by Use of Biologically Modified Embolic Devices: Coil-mediated Intraaneurysmal Delivery of Fibroblast Tissue Allografts

Endovascular Treatment of Experimental Aneurysms by Use of Biologically Modified Embolic Devices:... This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marx, W. F. Articles by Kallmes, D. F. Search for Related Content PubMed PubMed Citation Articles by Marx, W. F. Articles by Kallmes, D. F. Hotlight (NEW!) What's Hotlight? American Journal of Neuroradiology 22:323-333 (2 2001) © 2001 American Society of Neuroradiology ARTICLE Endovascular Treatment of Experimental Aneurysms by Use of Biologically Modified Embolic Devices: Coil-mediated Intraaneurysmal Delivery of Fibroblast Tissue Allografts William F. Marx ,a , Harry J. Cloft a , Gregory A. Helm a , John G. Short a , Huy M. Do a , Mary E. Jensen a and David F. Kallmes a a From the Departments of Radiology (W.F.M., J.G.S., M.E.J., D.F.K.) and Neurosurgery (G.H.), University of Virginia Health Sciences Center, Charlottesville, VA; the Department of Radiology (H.J.C.), Emory University, Atlanta, GA; and the Department of Radiology (H.M.D.), Stanford University, Palo Alto, CA. BACKGROUND AND PURPOSE: Our long-term goal is to improve intraaneurysmal fibrosis after aneurysm embolization, by implanting exogenous fibroblasts, using platinum coils. For the current project, we tested two hypotheses: 1) that exogenous, fluorescence-labeled rabbit fibroblast allografts remained viable and proliferated within rabbit carotid arteries, and 2) that these fibroblast allografts could be reliably implanted into experimental aneurysms by use of platinum coils. METHODS: Part 1. New Zealand White rabbit synovial fibroblasts obtained from a commercial vender were labeled with a fluorescent membrane marker. The common carotid arteries of New Zealand White rabbits were surgically exposed, ligated proximally and distally, and entered with 22-g angiocatheters. Through the angiocatheter we injected either phosphate-buffered saline-containing fluorescence-labeled fibroblasts (treatment vessels) or saline only (control vessels). The wounds were closed, and the subjects were kept alive for various time points up to 2 weeks. After sacrifice, the carotid artery segments were resected, processed for frozen-section histologic examination, and evaluated using epifluorescent microscopy and hematoxylin and eosin staining. Cell viability and proliferation were determined by comparing the treatment versus control vessels. Part 2. A) Fluorescence-labeled cells were grown in culture on platinum coils, which were then exposed to systemic arterial flow in the rabbit thoracic aorta for various lengths of time up to 40 minutes. The coil segments were then examined using fluorescent microscopy and the presence and relative amount of cells remaining on the coil were documented. B) Experimental aneurysms in rabbits were embolized with control platinum coils (n = 9) and platinum coils bearing rabbit synovial fibroblasts that were grown onto the coils in culture prior to implantation (n = 9). Subjects were sacrificed 3, 7, and 14 days after coil implantation. Histologic samples were studied to assess the presence or absence of nucleated cells within and around coil winds in order to determine whether fibroblasts had been successfully implanted into aneurysms. Data were evaluated using the chi-square test for statistical significance. RESULTS: Part 1. Fluorescence-labeled cells were examined in the treatment carotid artery segments and results were recorded at all time intervals. The treatment vessel segments showed evidence of progressive cellular proliferation, leading to complete vessel fibrosis at 2 weeks. Conversely, control vessel segments were filled predominately with unorganized thrombus at each time interval. Part 2. A) Numerous labeled fibroblasts remained adherent to the coil despite prolonged exposure to systemic arterial flow. B) Fibroblasts were seen adjacent to or within the central lumen of coils in eight (88%) of nine aneurysms treated with cell-bearing coils. Nucleated cells were not present in any of the nine control coil subjects. This represented a statistically significant difference ( P < .001). CONCLUSION: Fibroblast allografts remain viable and proliferate in the vascular space in rabbits. Furthermore, these same fibroblasts, after seeding onto platinum coils in culture, remain protected within the lumen of the coils and are retained within the coil lumen even after prolonged exposure to arterial blood flow. Coils can be used to deliver viable fibroblasts directly into experimental aneurysms successfully. These findings indicate that coil-mediated cell implantation is feasible and may be a potential method of increasing the biological activity of embolic coils. This article has been cited by other articles: I. Linfante, M.J. DeLeo III, M.J. Gounis, C.S. Brooks, and A.K. Wakhloo Cerecyte versus Platinum Coils in the Treatment of Intracranial Aneurysms: Packing Attenuation and Clinical and Angiographic Midterm Results AJNR Am. J. Neuroradiol., September 1, 2009; 30(8): 1496 - 1501. Abstract Full Text PDF C.T. Whitlow, C.P. Geer, C.W.T. Mattern, B.J. Mussat-Whitlow, S.K. Yazdani, J.L. Berry, J.H. Lalli, R.O. Claus, V.R. Challa, and P.P. Morris Endovascular Histologic Effects of Ultrathin Gold- or Vitronectin-Coated Platinum Aneurysm Coils in a Rodent Arterial Occlusion Model: A Preliminary Investigation AJNR Am. J. Neuroradiol., January 1, 2009; 30(1): 85 - 90. Abstract Full Text PDF D.A. Lewis, Y.H. Ding, D. Dai, R. Kadirvel, M.A. Danielson, H.J. Cloft, and D.F. Kallmes Morbidity and Mortality Associated with Creation of Elastase-Induced Saccular Aneurysms in a Rabbit Model AJNR Am. J. Neuroradiol., January 1, 2009; 30(1): 91 - 94. Abstract Full Text PDF T. Abruzzo, T. Tun, and A. Sambanis Efficient Transmicrocatheter Delivery of Functional Fibroblasts with a Bioengineered Collagen Gel-Platinum Microcoil Complex: Toward the Development of Endovascular Cell Therapy for Cerebral Aneurysms AJNR Am. J. Neuroradiol., September 1, 2007; 28(8): 1586 - 1593. Abstract Full Text PDF Y.H. Ding, D. Dai, M.A. Danielson, R. Kadirvel, D.A. Lewis, H.J. Cloft, and D.F. Kallmes Control of Aneurysm Volume by Adjusting the Position of Ligation During Creation of Elastase-Induced Aneurysms: A Prospective Study AJNR Am. J. Neuroradiol., May 1, 2007; 28(5): 857 - 859. Abstract Full Text PDF I. Linfante and A. K. Wakhloo Brain Aneurysms and Arteriovenous Malformations: Advancements and Emerging Treatments in Endovascular Embolization Stroke, April 1, 2007; 38(4): 1411 - 1417. Abstract Full Text PDF D. Dai, Y. H. Ding, M. A. Danielson, R. Kadirvel, L. W. Hunter, W.-Z. Zhan, G. A. Helm, D. A. Lewis, H. J. Cloft, G. C. Sieck, et al. Endovascular Treatment of Experimental Aneurysms by Use of Fibroblast-Coated Platinum Coils: An Angiographic and Histopathologic Study Stroke, January 1, 2007; 38(1): 170 - 176. Abstract Full Text PDF M. Bendszus and L. Solymosi Cerecyte Coils in the Treatment of Intracranial Aneurysms: A Preliminary Clinical Study AJNR Am. J. Neuroradiol., November 1, 2006; 27(10): 2053 - 2057. Abstract Full Text PDF Y.H. Ding, D. Dai, D.A. Lewis, M.A. Danielson, R. Kadirvel, J.N. Mandrekar, H.J. Cloft, and D.F. Kallmes Can neck size in elastase-induced aneurysms be controlled? A retrospective study. AJNR Am. J. Neuroradiol., September 1, 2006; 27(8): 1681 - 1684. Abstract Full Text PDF H. J. Cloft Have you been smoking something that is biologically active? AJNR Am. J. Neuroradiol., February 1, 2006; 27(2): 240 - 242. Full Text PDF D. Dai, Y.H. Ding, D.A. Lewis, and D.F. Kallmes A Proposed Ordinal Scale for Grading Histology in Elastase-Induced, Saccular Aneurysms AJNR Am. J. Neuroradiol., January 1, 2006; 27(1): 132 - 138. Abstract Full Text PDF D. Dai, Y. H. Ding, M. A. Danielson, R. Kadirvel, D. A. Lewis, H. J. Cloft, and D. F. Kallmes Histopathologic and Immunohistochemical Comparison of Human, Rabbit, and Swine Aneurysms Embolized with Platinum Coils AJNR Am. J. Neuroradiol., November 1, 2005; 26(10): 2560 - 2568. Abstract Full Text PDF Y. H. Ding, D. Dai, D. A. Lewis, M. A. Danielson, R. Kadirvel, J. N. Mandrekar, H. J. Cloft, and D. F. Kallmes Can Neck Size in Elastase-Induced Aneurysms Be Controlled? A Prospective Study AJNR Am. J. Neuroradiol., October 1, 2005; 26(9): 2364 - 2367. Abstract Full Text PDF I. Linfante, N. M. Akkawi, A. Perlow, V. Andreone, and A. K. Wakhloo Polyglycolide/Polylactide-Coated Platinum Coils for Patients With Ruptured and Unruptured Cerebral Aneurysms: A Single-Center Experience Stroke, September 1, 2005; 36(9): 1948 - 1953. Abstract Full Text PDF Y. H. Ding, D. Dai, D. A. Lewis, H. J. Cloft, and D. F. Kallmes Angiographic and Histologic Analysis of Experimental Aneurysms Embolized with Platinum Coils, Matrix, and HydroCoil AJNR Am. J. Neuroradiol., August 1, 2005; 26(7): 1757 - 1763. Abstract Full Text PDF M. Sluzewski, W. J. van Rooij, M. J. Slob, J. O. Bescos, C. H. Slump, and D. Wijnalda Relation between Aneurysm Volume, Packing, and Compaction in 145 Cerebral Aneurysms Treated with Coils Radiology, June 1, 2004; 231(3): 653 - 658. Abstract Full Text PDF Y. Murayama, S. Tateshima, N. R. Gonzalez, and F. Vinuela Matrix and Bioabsorbable Polymeric Coils Accelerate Healing of Intracranial Aneurysms: Long-Term Experimental Study Stroke, August 1, 2003; 34(8): 2031 - 2037. Abstract Full Text PDF D. F. Kallmes and N. H. Fujiwara New Expandable Hydrogel-Platinum Coil Hybrid Device for Aneurysm Embolization AJNR Am. J. Neuroradiol., October 1, 2002; 23(9): 1580 - 1588. Abstract Full Text PDF Home Subscribe Author Instructions Submit Online Search the AJNR Archives Feedback Help Copyright © 2010 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Neuroradiology American Journal of Neuroradiology

Endovascular Treatment of Experimental Aneurysms by Use of Biologically Modified Embolic Devices: Coil-mediated Intraaneurysmal Delivery of Fibroblast Tissue Allografts

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American Journal of Neuroradiology
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Copyright © 2010 by the American Society of Neuroradiology.
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0195-6108
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1936-959X
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Abstract

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marx, W. F. Articles by Kallmes, D. F. Search for Related Content PubMed PubMed Citation Articles by Marx, W. F. Articles by Kallmes, D. F. Hotlight (NEW!) What's Hotlight? American Journal of Neuroradiology 22:323-333 (2 2001) © 2001 American Society of Neuroradiology ARTICLE Endovascular Treatment of Experimental Aneurysms by Use of Biologically Modified Embolic Devices: Coil-mediated Intraaneurysmal Delivery of Fibroblast Tissue Allografts William F. Marx ,a , Harry J. Cloft a , Gregory A. Helm a , John G. Short a , Huy M. Do a , Mary E. Jensen a and David F. Kallmes a a From the Departments of Radiology (W.F.M., J.G.S., M.E.J., D.F.K.) and Neurosurgery (G.H.), University of Virginia Health Sciences Center, Charlottesville, VA; the Department of Radiology (H.J.C.), Emory University, Atlanta, GA; and the Department of Radiology (H.M.D.), Stanford University, Palo Alto, CA. BACKGROUND AND PURPOSE: Our long-term goal is to improve intraaneurysmal fibrosis after aneurysm embolization, by implanting exogenous fibroblasts, using platinum coils. For the current project, we tested two hypotheses: 1) that exogenous, fluorescence-labeled rabbit fibroblast allografts remained viable and proliferated within rabbit carotid arteries, and 2) that these fibroblast allografts could be reliably implanted into experimental aneurysms by use of platinum coils. METHODS: Part 1. New Zealand White rabbit synovial fibroblasts obtained from a commercial vender were labeled with a fluorescent membrane marker. The common carotid arteries of New Zealand White rabbits were surgically exposed, ligated proximally and distally, and entered with 22-g angiocatheters. Through the angiocatheter we injected either phosphate-buffered saline-containing fluorescence-labeled fibroblasts (treatment vessels) or saline only (control vessels). The wounds were closed, and the subjects were kept alive for various time points up to 2 weeks. After sacrifice, the carotid artery segments were resected, processed for frozen-section histologic examination, and evaluated using epifluorescent microscopy and hematoxylin and eosin staining. Cell viability and proliferation were determined by comparing the treatment versus control vessels. Part 2. A) Fluorescence-labeled cells were grown in culture on platinum coils, which were then exposed to systemic arterial flow in the rabbit thoracic aorta for various lengths of time up to 40 minutes. The coil segments were then examined using fluorescent microscopy and the presence and relative amount of cells remaining on the coil were documented. B) Experimental aneurysms in rabbits were embolized with control platinum coils (n = 9) and platinum coils bearing rabbit synovial fibroblasts that were grown onto the coils in culture prior to implantation (n = 9). Subjects were sacrificed 3, 7, and 14 days after coil implantation. Histologic samples were studied to assess the presence or absence of nucleated cells within and around coil winds in order to determine whether fibroblasts had been successfully implanted into aneurysms. Data were evaluated using the chi-square test for statistical significance. RESULTS: Part 1. Fluorescence-labeled cells were examined in the treatment carotid artery segments and results were recorded at all time intervals. The treatment vessel segments showed evidence of progressive cellular proliferation, leading to complete vessel fibrosis at 2 weeks. Conversely, control vessel segments were filled predominately with unorganized thrombus at each time interval. Part 2. A) Numerous labeled fibroblasts remained adherent to the coil despite prolonged exposure to systemic arterial flow. B) Fibroblasts were seen adjacent to or within the central lumen of coils in eight (88%) of nine aneurysms treated with cell-bearing coils. Nucleated cells were not present in any of the nine control coil subjects. This represented a statistically significant difference ( P < .001). CONCLUSION: Fibroblast allografts remain viable and proliferate in the vascular space in rabbits. Furthermore, these same fibroblasts, after seeding onto platinum coils in culture, remain protected within the lumen of the coils and are retained within the coil lumen even after prolonged exposure to arterial blood flow. Coils can be used to deliver viable fibroblasts directly into experimental aneurysms successfully. These findings indicate that coil-mediated cell implantation is feasible and may be a potential method of increasing the biological activity of embolic coils. This article has been cited by other articles: I. Linfante, M.J. DeLeo III, M.J. Gounis, C.S. Brooks, and A.K. Wakhloo Cerecyte versus Platinum Coils in the Treatment of Intracranial Aneurysms: Packing Attenuation and Clinical and Angiographic Midterm Results AJNR Am. J. Neuroradiol., September 1, 2009; 30(8): 1496 - 1501. Abstract Full Text PDF C.T. Whitlow, C.P. Geer, C.W.T. Mattern, B.J. Mussat-Whitlow, S.K. Yazdani, J.L. Berry, J.H. Lalli, R.O. Claus, V.R. Challa, and P.P. Morris Endovascular Histologic Effects of Ultrathin Gold- or Vitronectin-Coated Platinum Aneurysm Coils in a Rodent Arterial Occlusion Model: A Preliminary Investigation AJNR Am. J. Neuroradiol., January 1, 2009; 30(1): 85 - 90. Abstract Full Text PDF D.A. Lewis, Y.H. Ding, D. Dai, R. Kadirvel, M.A. Danielson, H.J. Cloft, and D.F. Kallmes Morbidity and Mortality Associated with Creation of Elastase-Induced Saccular Aneurysms in a Rabbit Model AJNR Am. J. Neuroradiol., January 1, 2009; 30(1): 91 - 94. Abstract Full Text PDF T. Abruzzo, T. Tun, and A. Sambanis Efficient Transmicrocatheter Delivery of Functional Fibroblasts with a Bioengineered Collagen Gel-Platinum Microcoil Complex: Toward the Development of Endovascular Cell Therapy for Cerebral Aneurysms AJNR Am. J. Neuroradiol., September 1, 2007; 28(8): 1586 - 1593. Abstract Full Text PDF Y.H. Ding, D. Dai, M.A. Danielson, R. Kadirvel, D.A. Lewis, H.J. Cloft, and D.F. Kallmes Control of Aneurysm Volume by Adjusting the Position of Ligation During Creation of Elastase-Induced Aneurysms: A Prospective Study AJNR Am. J. Neuroradiol., May 1, 2007; 28(5): 857 - 859. Abstract Full Text PDF I. Linfante and A. K. Wakhloo Brain Aneurysms and Arteriovenous Malformations: Advancements and Emerging Treatments in Endovascular Embolization Stroke, April 1, 2007; 38(4): 1411 - 1417. Abstract Full Text PDF D. Dai, Y. H. Ding, M. A. Danielson, R. Kadirvel, L. W. Hunter, W.-Z. Zhan, G. A. Helm, D. A. Lewis, H. J. Cloft, G. C. Sieck, et al. Endovascular Treatment of Experimental Aneurysms by Use of Fibroblast-Coated Platinum Coils: An Angiographic and Histopathologic Study Stroke, January 1, 2007; 38(1): 170 - 176. Abstract Full Text PDF M. Bendszus and L. Solymosi Cerecyte Coils in the Treatment of Intracranial Aneurysms: A Preliminary Clinical Study AJNR Am. J. Neuroradiol., November 1, 2006; 27(10): 2053 - 2057. Abstract Full Text PDF Y.H. Ding, D. Dai, D.A. Lewis, M.A. Danielson, R. Kadirvel, J.N. Mandrekar, H.J. Cloft, and D.F. Kallmes Can neck size in elastase-induced aneurysms be controlled? A retrospective study. AJNR Am. J. Neuroradiol., September 1, 2006; 27(8): 1681 - 1684. Abstract Full Text PDF H. J. Cloft Have you been smoking something that is biologically active? AJNR Am. J. Neuroradiol., February 1, 2006; 27(2): 240 - 242. Full Text PDF D. Dai, Y.H. Ding, D.A. Lewis, and D.F. Kallmes A Proposed Ordinal Scale for Grading Histology in Elastase-Induced, Saccular Aneurysms AJNR Am. J. Neuroradiol., January 1, 2006; 27(1): 132 - 138. Abstract Full Text PDF D. Dai, Y. H. Ding, M. A. Danielson, R. Kadirvel, D. A. Lewis, H. J. Cloft, and D. F. Kallmes Histopathologic and Immunohistochemical Comparison of Human, Rabbit, and Swine Aneurysms Embolized with Platinum Coils AJNR Am. J. Neuroradiol., November 1, 2005; 26(10): 2560 - 2568. Abstract Full Text PDF Y. H. Ding, D. Dai, D. A. Lewis, M. A. Danielson, R. Kadirvel, J. N. Mandrekar, H. J. Cloft, and D. F. Kallmes Can Neck Size in Elastase-Induced Aneurysms Be Controlled? A Prospective Study AJNR Am. J. Neuroradiol., October 1, 2005; 26(9): 2364 - 2367. Abstract Full Text PDF I. Linfante, N. M. Akkawi, A. Perlow, V. Andreone, and A. K. Wakhloo Polyglycolide/Polylactide-Coated Platinum Coils for Patients With Ruptured and Unruptured Cerebral Aneurysms: A Single-Center Experience Stroke, September 1, 2005; 36(9): 1948 - 1953. Abstract Full Text PDF Y. H. Ding, D. Dai, D. A. Lewis, H. J. Cloft, and D. F. Kallmes Angiographic and Histologic Analysis of Experimental Aneurysms Embolized with Platinum Coils, Matrix, and HydroCoil AJNR Am. J. Neuroradiol., August 1, 2005; 26(7): 1757 - 1763. Abstract Full Text PDF M. Sluzewski, W. J. van Rooij, M. J. Slob, J. O. Bescos, C. H. Slump, and D. Wijnalda Relation between Aneurysm Volume, Packing, and Compaction in 145 Cerebral Aneurysms Treated with Coils Radiology, June 1, 2004; 231(3): 653 - 658. Abstract Full Text PDF Y. Murayama, S. Tateshima, N. R. Gonzalez, and F. Vinuela Matrix and Bioabsorbable Polymeric Coils Accelerate Healing of Intracranial Aneurysms: Long-Term Experimental Study Stroke, August 1, 2003; 34(8): 2031 - 2037. Abstract Full Text PDF D. F. Kallmes and N. H. Fujiwara New Expandable Hydrogel-Platinum Coil Hybrid Device for Aneurysm Embolization AJNR Am. J. Neuroradiol., October 1, 2002; 23(9): 1580 - 1588. Abstract Full Text PDF Home Subscribe Author Instructions Submit Online Search the AJNR Archives Feedback Help Copyright © 2010 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X

Journal

American Journal of Neuroradiology – American Journal of Neuroradiology

Published: Feb 1, 2001

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