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Effect of solution viscosity on generating long DNA nanostrands array via patterned molecular combing

Abstract

In this study, we have investigated the effect of solution viscosity on generating the aligned and long DNA nanostrands array via modified molecular combing/soft lithography technique (or patterned molecular combing). By using the solution with higher viscosity, a low peeling-off speed can be applied to generate the long DNA nanostrands and the thornlike structures can be eliminated effectively. The DNA nanostrands array can also be generated by utilizing the polydimethylsiloxane stamp having microwells with larger diameter; however, variation of the lengths of the DNA nanostrands becomes greater as the solution viscosity and the size of microwells increase.

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Journal

Applied Physics LettersAmerican Institute of Physics

Published: Jun 15, 2009

Keywords: biological techniques; DNA; molecular biophysics; nanobiotechnology; soft lithography; viscosity

DOI: 10.1063/1.3157165

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