Hydroxyurea and Escherichia coli Nucleoside Diphosphate Reductase
Abstract
Upon removal of hydroxyurea, bacteria regained the ability to synthesize DNA. This occurred in the absence of concomitant protein production. The results indicate that irreversible inactivation of nucleoside diphosphate reductase followed by rapid regeneration of the enzyme upon removal of the drug is not the basis of the selective and reversible inhibition of DNA synthesis by hydroxyurea. 1 This investigation was supported by a grant from the Damon Runyon Memorial Fund for Cancer Research. 2 Research Career Development Awardee of the Division of General Medical Sciences, USPHS (5-K3-GM-29,024).