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A Novel Ribonucleoprotein Complex Defined by Monoclonal Antibodies to NIH 3T3 Cells Transfected with Human Pancreatic Adenocarcinoma DNA

A Novel Ribonucleoprotein Complex Defined by Monoclonal Antibodies to NIH 3T3 Cells Transfected... Three monoclonal antibodies elicited to NIH 3T3 cells transfected with DNA from a human pancreatic adenocarcinoma cell line recognized a novel ribonucleoprotein complex. Minimally, this ribonucleoprotein complex contained a M r 240,000 protein (by sodium didecyl sulfate-polyacrylamide gel electrophoresis) and two RNA species with apparent sizes of 1.5 and 3.0 kilobases (by formaldehyde agarose gel electrophoresis). In addition to a cytoplasmic and nuclear subcellular localization, the RNA antigen was secreted from human tumor cell lines and NIH 3T3 cells transfected with pancreatic tumor DNA (inhibitable by monensin) and was apparently not a viral or Mycoplasma contaminant. The ribonucleoprotein antigen was detected in some normal tissues by immunoperoxidase but was not found in or secreted from in vitro cultured normal human fibroblasts, nontransfected or spontaneously transformed NIH 3T3 cells, or normal peripheral blood leukocytes. 1 This work was supported by grant CA42114 from the NIH. 2 To whom requests for reprints should be addressed, at Box 3839, Duke University Medical Center, Durham, NC 27710. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cancer Research American Association of Cancer Research

A Novel Ribonucleoprotein Complex Defined by Monoclonal Antibodies to NIH 3T3 Cells Transfected with Human Pancreatic Adenocarcinoma DNA

A Novel Ribonucleoprotein Complex Defined by Monoclonal Antibodies to NIH 3T3 Cells Transfected with Human Pancreatic Adenocarcinoma DNA

Cancer Research , Volume 50 (18): 6015 – Sep 15, 1990

Abstract

Three monoclonal antibodies elicited to NIH 3T3 cells transfected with DNA from a human pancreatic adenocarcinoma cell line recognized a novel ribonucleoprotein complex. Minimally, this ribonucleoprotein complex contained a M r 240,000 protein (by sodium didecyl sulfate-polyacrylamide gel electrophoresis) and two RNA species with apparent sizes of 1.5 and 3.0 kilobases (by formaldehyde agarose gel electrophoresis). In addition to a cytoplasmic and nuclear subcellular localization, the RNA antigen was secreted from human tumor cell lines and NIH 3T3 cells transfected with pancreatic tumor DNA (inhibitable by monensin) and was apparently not a viral or Mycoplasma contaminant. The ribonucleoprotein antigen was detected in some normal tissues by immunoperoxidase but was not found in or secreted from in vitro cultured normal human fibroblasts, nontransfected or spontaneously transformed NIH 3T3 cells, or normal peripheral blood leukocytes. 1 This work was supported by grant CA42114 from the NIH. 2 To whom requests for reprints should be addressed, at Box 3839, Duke University Medical Center, Durham, NC 27710.

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Publisher
American Association of Cancer Research
Copyright
Copyright © 1990 by the American Association for Cancer Research.
ISSN
0008-5472
Publisher site

Abstract

Three monoclonal antibodies elicited to NIH 3T3 cells transfected with DNA from a human pancreatic adenocarcinoma cell line recognized a novel ribonucleoprotein complex. Minimally, this ribonucleoprotein complex contained a M r 240,000 protein (by sodium didecyl sulfate-polyacrylamide gel electrophoresis) and two RNA species with apparent sizes of 1.5 and 3.0 kilobases (by formaldehyde agarose gel electrophoresis). In addition to a cytoplasmic and nuclear subcellular localization, the RNA antigen was secreted from human tumor cell lines and NIH 3T3 cells transfected with pancreatic tumor DNA (inhibitable by monensin) and was apparently not a viral or Mycoplasma contaminant. The ribonucleoprotein antigen was detected in some normal tissues by immunoperoxidase but was not found in or secreted from in vitro cultured normal human fibroblasts, nontransfected or spontaneously transformed NIH 3T3 cells, or normal peripheral blood leukocytes. 1 This work was supported by grant CA42114 from the NIH. 2 To whom requests for reprints should be addressed, at Box 3839, Duke University Medical Center, Durham, NC 27710.

Journal

Cancer ResearchAmerican Association of Cancer Research

Published: Sep 15, 1990

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