Nonchromatographic radioimmunoassay of unconjugated estriol in plasma, with polyethylene glycol as precipitant.

Nonchromatographic radioimmunoassay of unconjugated estriol in plasma, with polyethylene glycol... Abstract We describe a rapid, reliable radioimmunoassay for unconjugated estriol in plasma. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay is sensitive (25 pg for standards), precise, and accurate. At high and low concentrations of estriol, intra-assay coefficients of variation were 7.1% and 7.6%, respectively, and inter-assay coefficients of variation were 7.2% and 10.0 %, respectively. Free (3H) estriol is not precipitated by polyethylene glycol. This radioimmunoassay of estriol, with a highly specific antiserum and with polyethylene glycol as the antibody precipitant, is a reliable one-day assay that is practical both for the clinical laboratory and the obstetrician. © 1976 The American Association for Clinical Chemistry « Previous | Next Article » Table of Contents This Article Clinical Chemistry March 1976 vol. 22 no. 3 359-363 » Abstract PDF Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Responses No responses published Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Schiller, H. S. Articles by Brammall, M. A. Search for related content PubMed PubMed citation Articles by Schiller, H. S. Articles by Brammall, M. A. Related Content Load related web page information Follow Us Clinical Chemistry Trainee Council Register Today! www.traineecouncil.org Information for Authors Submit a Manuscript Editorial Board Clinical Case Studies Clinical Chemistry Guide to Scientific Writing Journal Club Podcasts Translated Content Annual Meeting Abstracts Permissions and Reprints Advertising Copyright © 2012 by the American Association for Clinical Chemistry http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry American Association for Clinical Chemistry

Nonchromatographic radioimmunoassay of unconjugated estriol in plasma, with polyethylene glycol as precipitant.

Clinical Chemistry, Volume 22 (3): 359 – Mar 1, 1976

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Publisher
American Association for Clinical Chemistry
Copyright
Copyright © 1976 by the American Association for Clinical Chemistry.
ISSN
0009-9147
eISSN
1530-8561
Publisher site
See Article on Publisher Site

Abstract

Abstract We describe a rapid, reliable radioimmunoassay for unconjugated estriol in plasma. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay is sensitive (25 pg for standards), precise, and accurate. At high and low concentrations of estriol, intra-assay coefficients of variation were 7.1% and 7.6%, respectively, and inter-assay coefficients of variation were 7.2% and 10.0 %, respectively. Free (3H) estriol is not precipitated by polyethylene glycol. This radioimmunoassay of estriol, with a highly specific antiserum and with polyethylene glycol as the antibody precipitant, is a reliable one-day assay that is practical both for the clinical laboratory and the obstetrician. © 1976 The American Association for Clinical Chemistry « Previous | Next Article » Table of Contents This Article Clinical Chemistry March 1976 vol. 22 no. 3 359-363 » Abstract PDF Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Responses No responses published Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Schiller, H. S. Articles by Brammall, M. A. Search for related content PubMed PubMed citation Articles by Schiller, H. S. Articles by Brammall, M. A. Related Content Load related web page information Follow Us Clinical Chemistry Trainee Council Register Today! www.traineecouncil.org Information for Authors Submit a Manuscript Editorial Board Clinical Case Studies Clinical Chemistry Guide to Scientific Writing Journal Club Podcasts Translated Content Annual Meeting Abstracts Permissions and Reprints Advertising Copyright © 2012 by the American Association for Clinical Chemistry

Journal

Clinical ChemistryAmerican Association for Clinical Chemistry

Published: Mar 1, 1976

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