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Small Molecule Quantification by Liquid Chromatography-Mass Spectrometry for Metabolites of Drugs and Drug Candidates

Small Molecule Quantification by Liquid Chromatography-Mass Spectrometry for Metabolites of Drugs and Drug Candidates Small Molecule Quantification by Liquid Chromatography-Mass Spectrometry for Metabolites of Drugs and Drug Candidates var callbackToken='5053BDAA1C74B56'; Skip to main page content HOME CURRENT ISSUE ARCHIVE FEEDBACK SUBSCRIPTIONS ALERTS HELP Keywords GO Advanced » Institution: DeepDyve Sign In as Member / Individual User Name Password Sign In Small Molecule Quantification by Liquid Chromatography-Mass Spectrometry for Metabolites of Drugs and Drug Candidates Upendra P. Dahal , Jeffrey P. Jones , John A. Davis and Dan A. Rock Department of Chemistry, Washington State University, Pullman, Washington (U.P.D., J.P.J.); and Department of Pharmacokinetics and Drug Metabolism, Amgen Inc., Seattle, Washington (J.A.D., D.A.R.) <h3>Address correspondence to:</h3> Jeffrey P. Jones, Fulmer 406, Department of Chemistry, Washington State University, Pullman, WA 99164-4630. E-mail: jpj@wsu.edu <h2>Abstract</h2> Identification and quantification of the metabolites of drugs and drug candidates are routinely performed using liquid chromatography-mass spectrometry (LC-MS). The best practice is to generate a standard curve with the metabolite versus the internal standard. However, to avoid the difficulties in metabolite synthesis, standard curves are sometimes prepared using the substrate, assuming that the signal for substrate and the metabolite will be equivalent. We have tested the errors associated with this assumption using a series of very similar compounds that http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Drug Metabolism and Disposition Am. Soc for Pharma & Experimental Therapeutics

Small Molecule Quantification by Liquid Chromatography-Mass Spectrometry for Metabolites of Drugs and Drug Candidates

Abstract

Small Molecule Quantification by Liquid Chromatography-Mass Spectrometry for Metabolites of Drugs and Drug Candidates var callbackToken='5053BDAA1C74B56'; Skip to main page content HOME CURRENT ISSUE ARCHIVE FEEDBACK SUBSCRIPTIONS ALERTS HELP Keywords GO Advanced » Institution: DeepDyve Sign In as Member / Individual User Name Password Sign In Small Molecule Quantification by Liquid Chromatography-Mass Spectrometry for Metabolites of Drugs and Drug Candidates Upendra P. Dahal , Jeffrey P. Jones , John A. Davis and Dan A. Rock Department of Chemistry, Washington State University, Pullman, Washington (U.P.D., J.P.J.); and Department of Pharmacokinetics and Drug Metabolism, Amgen Inc., Seattle, Washington (J.A.D., D.A.R.) <h3>Address correspondence to:</h3> Jeffrey P. Jones, Fulmer 406, Department of Chemistry, Washington State University, Pullman, WA 99164-4630. E-mail: jpj@wsu.edu <h2>Abstract</h2> Identification and quantification of the metabolites of drugs and drug candidates are routinely performed using liquid chromatography-mass spectrometry (LC-MS). The best practice is to generate a standard curve with the metabolite versus the internal standard. However, to avoid the difficulties in metabolite synthesis, standard curves are sometimes prepared using the substrate, assuming that the signal for substrate and the metabolite will be equivalent. We have tested the errors associated with this assumption using a series of very similar compounds that
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