Link Between the Adult and the Metacercaria of Clinostomum heluans Braun, 1899 (Trematoda: Clinostomidae) Through DNA Sequences, and its Phylogenetic Position within the Genus Clinostomum Leidy, 1856

Link Between the Adult and the Metacercaria of Clinostomum heluans Braun, 1899 (Trematoda:... The phylogenetic position of Clinostomum heluans Braun, 1899 within the genus Clinostomum Leidy, 1856 is reported in this study based on sequences of the barcoding region of the mitochondrial cytochrome c oxidase subunit 1 gene (COX1). Additionally, molecular data are used to link the adult and the metacercariae of the species. The metacercariae of C. heluans were found encysted infecting the cichlid fish Australoheros sp. in Minas Gerais, Brazil, whereas the adults were obtained from the mouth cavity of the Great White egret Ardea alba, in Campeche, Mexico. The COX1sequences obtained for the Mexican clinostomes and the Brazilian metacercaria were almost identical (0.2% of molecular divergence), indicating conspecificity. Similar molecular divergence (0.2-0.4%) were found between sequences of C. heluans here reported and Clinostomum sp. 6 (after Locke et al., 2015) previously obtained from a metacercaria recovered from the cichlid Cichlasoma boliviense, in Santa Cruz, Bolivia. Both, Maximum Likelihood (ML) and Bayesian Inference (BI) analyses unequivocally show the conspecificity between C. heluans and Clinostomum sp. 6, which form a monophyletic clade with high nodal support and very low genetic divergence. Moreover, tree topology reveals that C. heluans occupies a basal position with respect to New World species of Clinostomum, although a more dense taxon sampling of species within the genus is further required. The metacercaria of C. heluans seems to be specific to cichlid fish because both samples from South America were recovered from species of this fish family, although not closely related. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Parasitology Allen Press

Link Between the Adult and the Metacercaria of Clinostomum heluans Braun, 1899 (Trematoda: Clinostomidae) Through DNA Sequences, and its Phylogenetic Position within the Genus Clinostomum Leidy, 1856

,; ,; ,; ,; ,; ,
The Journal of Parasitology : 1 – Mar 9, 2018

Loading next page...
 
/lp/allen_press/link-between-the-adult-and-the-metacercaria-of-clinostomum-heluans-JhTlQ6YEtu
Publisher
American Society of Parasitologists
ISSN
0022-3395
eISSN
1937-2345
D.O.I.
10.1645/17-183
Publisher site
See Article on Publisher Site

Abstract

The phylogenetic position of Clinostomum heluans Braun, 1899 within the genus Clinostomum Leidy, 1856 is reported in this study based on sequences of the barcoding region of the mitochondrial cytochrome c oxidase subunit 1 gene (COX1). Additionally, molecular data are used to link the adult and the metacercariae of the species. The metacercariae of C. heluans were found encysted infecting the cichlid fish Australoheros sp. in Minas Gerais, Brazil, whereas the adults were obtained from the mouth cavity of the Great White egret Ardea alba, in Campeche, Mexico. The COX1sequences obtained for the Mexican clinostomes and the Brazilian metacercaria were almost identical (0.2% of molecular divergence), indicating conspecificity. Similar molecular divergence (0.2-0.4%) were found between sequences of C. heluans here reported and Clinostomum sp. 6 (after Locke et al., 2015) previously obtained from a metacercaria recovered from the cichlid Cichlasoma boliviense, in Santa Cruz, Bolivia. Both, Maximum Likelihood (ML) and Bayesian Inference (BI) analyses unequivocally show the conspecificity between C. heluans and Clinostomum sp. 6, which form a monophyletic clade with high nodal support and very low genetic divergence. Moreover, tree topology reveals that C. heluans occupies a basal position with respect to New World species of Clinostomum, although a more dense taxon sampling of species within the genus is further required. The metacercaria of C. heluans seems to be specific to cichlid fish because both samples from South America were recovered from species of this fish family, although not closely related.

Journal

The Journal of ParasitologyAllen Press

Published: Mar 9, 2018

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial