TY - JOUR AU1 - Zeng, Ruixia AU2 - Fang, Yan AU3 - Zhang, Yibo AU4 - Bai, Shuling AB - Background: Obesity is closely related to the abnormal differentiation of adipocytes, which are subjected to high plasma levels of free fatty acids (FFAs). As the most abundant FFA in the bloodstream, oleic acid (OA) has the ability to induce adipogenic differentiation in human adipose-derived stromal cells (hADSCs). Recently, p62, an autophagy mediator, has been shown to play a role in obesity and adipose tissue metabolism. Therefore, the aim of this study was to investigate the roles of autophagy and mitochondrial function at different stages of OA (in combination with insulin and dexamethasone)-induced adipogenesis in hADSCs. Methods: The hADSCs were incubated with OA, insulin, and dexamethasone after pretreatment with autophagy inhibitors or knockdown of p62 with shRNA. The adiposeness level was then analyzed by oil red O staining in the cells. The related proteins or mRNA levels were detected by western blot analysis or quantitative real-time polymerase chain reaction (PCR). Results: Treatment with 80 μM OA (substituted for isobutylmethylxantine; IBMX) for 10 days successfully induced hADSCs to adipocytes. During OA-induced adipogenesis, autophagy was induced, with an increased LC3II/I ratio on day 3 andadecreasedprotein levelofp62on andafter day3.Inhibition of autophagy with 3-methyladenine (3MA) at the early stage (day 0 to day 3) TI - p62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells JF - Lipids in Health and Disease DO - 10.1186/s12944-018-0733-5 DA - 2018-06-04 UR - https://www.deepdyve.com/lp/springer-journals/p62-is-linked-to-mitophagy-in-oleic-acid-induced-adipogenesis-in-human-si8P5wYeNm SP - 1 EP - 10 VL - 17 IS - 1 DP - DeepDyve ER -