TY - JOUR
AU1 - Rumyantsev, A.
AU2 - Zakharov, G.
AU3 - Zhuravlev, A.
AU4 - Padkina, M.
AU5 - Savvateeva-Popova, E.
AU6 - Sambuk, E.
AB - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs’ and RNA-binding proteins’ interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-UTRs were functionally active and recognized in yeast. Therefore, yeast might be used as an appropriate model system for studies of 3′-UTR’s role in post-transcriptional regulation.
TI - Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae
JF - Russian Journal of Genetics
DO - 10.1134/S102279541406009X
DA - 2014-06-21
UR - https://www.deepdyve.com/lp/springer-journals/expression-of-the-drosophila-melanogaster-limk1-gene-3-utrs-mrna-in-q0WwoTLwMr
SP - 569
EP - 576
VL - 50
IS - 6
DP - DeepDyve
ER -