TY - JOUR AU1 - Zothanpuia, AU2 - Passari, Ajit AU3 - Leo, Vincent AU4 - Chandra, Preeti AU5 - Kumar, Brijesh AU6 - Nayak, Chandra AU7 - Hashem, Abeer AU8 - Abd_Allah, Elsayed AU9 - Alqarawi, Abdulaziz AU1 - Singh, Bhim AB - Background: Actinobacteria from freshwater habitats have been explored less than from other habitats in the search for compounds of pharmaceutical value. This study highlighted the abundance of actinobacteria from freshwater sediments of two rivers and one lake, and the isolates were studied for their ability to produce antimicrobial bioactive compounds. Results: 16S rRNA gene sequencing led to the identification of 84 actinobacterial isolates separated into a com- mon genus (Streptomyces) and eight rare genera (Nocardiopsis, Saccharopolyspora, Rhodococcus, Prauserella, Amyco- latopsis, Promicromonospora, Kocuria and Micrococcus). All strains that showed significant inhibition potentials were found against Gram-positive, Gram-negative and yeast pathogens. Further, three biosynthetic genes, polyketide synthases type II (PKS II), nonribosomal peptide synthetases (NRPS) and aminodeoxyisochorismate synthase (phzE), were detected in 38, 71 and 29% of the strains, respectively. Six isolates based on their antimicrobial potentials were selected for the detection and quantification of standard antibiotics using ultra performance liquid chromatography (UPLC–ESI–MS/MS) and volatile organic compounds ( VOCs) using gas chromatography mass spectrometry (GC/MS). Four antibiotics (fluconazole, trimethoprim, ketoconazole and rifampicin) and 35 VOCs were quantified and deter - mined from the methanolic crude extract of six selected Streptomyces strains. Conclusion: Infectious diseases still remain one of the leading causes TI - Bioprospection of actinobacteria derived from freshwater sediments for their potential to produce antimicrobial compounds JF - Microbial Cell Factories DO - 10.1186/s12934-018-0912-0 DA - 2018-05-05 UR - https://www.deepdyve.com/lp/springer-journals/bioprospection-of-actinobacteria-derived-from-freshwater-sediments-for-j0OhWxZ6e0 SP - 1 EP - 14 VL - 17 IS - 1 DP - DeepDyve ER -