TY - JOUR
AU1 - Foote, Christopher S.
AU2 - Goyne, Thomas E.
AU3 - Lehrer, Robert I.
AB - On phagocytosing a microorganism, the neutrophil zpolymor-phonuclear leukocyte, PMN) consumes oxygen at a sharply elevated rate1. The oxygen is used to kill the microorganism, presumably being used to produce a potent oxidizing agent or agents. Candidates for these bactericidal agents are singlet oxygen, hydroxyl radical and chlorinating agents (that is, species containing ‘active’ Cl in a formal +1 oxidation state: HOCl, Cl2, N-chloroamides, and so on)1–5. We now report a semiquantitative assay for PMN-generated active chlorine based on its trapping with 1,3,5-trimethoxybenzene (TMB). Using this assay, we have found that at least 28% of the oxygen consumed by stimulated normal human PMNs is converted to active chlorinating agents.
TI - Assessment of chlorination by human neutrophils
JF - Nature
DO - 10.1038/301715a0
DA - 1983-02-24
UR - https://www.deepdyve.com/lp/springer-journals/assessment-of-chlorination-by-human-neutrophils-gq00YgkVTa
SP - 715
EP - 716
VL - 301
IS - 5902
DP - DeepDyve
ER -