TY - JOUR
AU1 - van Rossum, Denise
AU2 - Hilbert, Sören
AU3 - Straßenburg, Silke
AU4 - Hanisch, Uwe‐karsten
AU5 - Brück, Wolfgang
AB - Macrophages are key effectors in demyelinating diseases of the central and peripheral nervous system by phagocytosing myelin and releasing immunoregulatory mediators. Here, we report on a distinct, a priori anti‐inflammatory reaction of macrophages phagocytosing myelin upon contact with damaged nerve tissue. Macrophages rapidly invaded peripheral (sciatic) and central (optic) nerve tissues in vitro, readily incorporated myelin and expressed high levels of phagocytosis‐associated molecules (e.g., Fc and scavenger receptors). In contrast, factors involved in antigen presentation (MHC class‐II, CD80, CD86) revealed only a restricted expression. In parallel, a highly ordered appearance of cytokines and chemokines was detected. IL‐10, IL‐6, CCL22, and CXCL1 were immediately but transiently induced, whereas CCL2, CCL11, and TGFβ revealed more persisting levels. Such a profile would attract neutrophils, monocytes/macrophages, and Th2 cells as well as bias for a Th2‐supporting environment. Importantly, proinflammatory/Th1‐supporting factors, such as TNFα, IL‐12p70, CCL3, and CCL5, were not induced. Still the simultaneous presence of TGFβ and IL‐6 could assist Th17 development, further depending on yet not present IL‐23. The release pattern was clearly distinct from reactive phenotypes induced in isolated macrophages and microglia upon treatment with IL‐4, IL‐13, bacterial lipopolysaccharide, IFNγ, or purified myelin. Nerve‐exposed macrophages thus commit to a unique functional orientation. © 2007 Wiley‐Liss, Inc.
TI - Myelin‐phagocytosing macrophages in isolated sciatic and optic nerves reveal a unique reactive phenotype
JF - Glia
DO - 10.1002/glia.20611
DA - 2008-02-01
UR - https://www.deepdyve.com/lp/wiley/myelin-phagocytosing-macrophages-in-isolated-sciatic-and-optic-nerves-fMmCU1Yy3Q
SP - 271
EP - 283
VL - 56
IS - 3
DP - DeepDyve
ER -